基于网络药理学探究栀子的治疗潜能及作用机制
Exploring the Therapeutic Potential and Mechanism of Action of Gardenia jasminoides Based on Network Pharmacology

目的：基于网络药理学手段探究栀子对疾病的作用机制和治疗潜能。方法：采用TCMSP数据库对栀子的活性成分和治疗靶点进行预测和筛选，随后去重。Cytoscape 3.9.0绘制了活性组分和靶标网络，并筛选出核心靶点，String数据库分析了蛋白–蛋白相互作用(PPI)，去重后的靶点输入到DAVID数据库中查询其涉及的信号通路，进行GO和KEGG通路富集分析。最后将查询的信号通路输入CTD数据库，筛选出相应疾病，栀子的“成分–靶点–通路–疾病”网络图利用Cytoscape 3.9.0软件进行构建，并对其作用机制分析。结果：筛选出98个化学成分，去重后靶点保留了248个，这些靶点涉及了188条通路，参与了多种疾病，本文主要对6种疾病进行分析。结论：通过网络药理学发现，栀子对多种疾病具有治疗潜能，其中用KEGG前10条通路进行疾病分析，发现对癌瘤、前列腺肿瘤、腺癌、乳腺肿瘤、肝硬化、胃癌的频次较多，此外，对阿尔茨海默病、神经变性等也有一定的作用，这都为后续深入探究栀子作为新药的研究提供了基础。
Aims: To investigate the mechanism of action and therapeutic potential of Gardenia jasminoides for disease based on network pharmacological tools. Methods: The TCMSP database was used to predict and screen the active components and therapeutic targets of gardenia, followed by de-duplication. Cytoscape 3.9.0 mapped the active components and target networks and screened the core targets, String database was analyzed for Protein-Protein Interactions (PPI), and the deduplicated targets were entered into the DAVID database to query the signaling pathways they were involved in the GO and KEGG pathways were enriched for analysis. Finally, the queried signalling pathways were entered into the CTD database to screen out the corresponding diseases. The network map was constructed using Cytoscape 3.9.0 software, and the mechanism of action was analysed. Results: 98 chemical components were screened and 248 targets were retained after de-duplication. These targets involved 188 pathways and were involved in a variety of diseases. In this paper, 6 kinds of diseases were mainly analysed. Conclusion: Gardenia jasminoides was found to have therapeutic potential for a variety of diseases through network pharmacology, where disease analysis using the first 10 pathways of KEGG revealed a higher frequency for carcinoma, prostate tumour, adenocarcinoma, breast tumour, liver cirrhosis and gastric cancer. In addition, it also has a certain effect on Alzheimer’s disease, neurodegeneration, etc. All these provide a basis for subsequent indepth exploration of gardenia as a new drug.