Introduction:The Central African Republic is one of the 30 high
Tuberculosis burden countries in the world, with an incidence of 540 cases per
100,000 population and a mortality of 91 deaths per 100,000 population. Since
2020, following WHO recommendations, the
National Reference Laboratory for Tuberculosis has been using the Xpert® MTB/RIF assay as a first-line diagnostic test
for the early detection ofDrug Resistance Tuberculosis.
The goal of this study was to evaluate the contribution of
the Xpert® MTB/RIF assay to the surveillance of
rifampicin resistance in new and previously treated tuberculosis cases. Materials and Methods: The
data relative to the Xpert® MTB/RIF assay carried out on various categories of
tuberculosis patients registered at the National Reference Laboratory for
Tuberculosis in 2020 were analyzed retrospectively. The
categories of tuberculosis patients were new cases, failed treatment cases,
relapse cases, lost-to-follow-up casesand multidrug-resistant tuberculosis contact cases. Results: A
total of 1404 tuberculosis patients were registered at the NRL-TB in 2020; the
mean age was 39.2 years (2
References
[1]
Tritar, F., Daghfous, H., Ben Saad, S. and Slim-Saidi, L. (2015) Management of Multidrug-Resistant Tuberculosis. Revue de Pneumologie Clinique, 71, 130-139. https://doi.org/10.1016/j.pneumo.2014.05.001
[2]
World Health Organization (2020) Global Tuberculosis Report 2020. https://www.who.int/publications/i/item/9789240013131
[3]
Chakaya, J., Ntoumi, F., Aklillu, E., Fatima, R., Mwaba, P., Kapata, N., Mfinanga, S., Hasnain, S.E., Katoto, P., Bulabula, A., Sam-Agudu, N.A., Nachega, J.B., Tiberi, S., McHugh, T.D., Abubakar, I. and Zumla, A. (2021) Global Tuberculosis Report 2020—Reflections on the Global TB Burden, Treatment and Prevention Efforts. International Journal of Infectious Diseases, 113, S7-S12. https://doi.org/10.1016/j.ijid.2021.02.107
[4]
Araya, G.W., Tsehaye, A.D. and Genet, G.H. (2020). Frequency of MTB and Rifampicin Resistance MTB Using Xpert-MTB/RIF Assay among Adult Presumptive Tuberculosis Patients in Tigray, Northern Ethiopia: A Cross Sectional Study. PLOS ONE, 15, e0240361. https://doi.org/10.1371/journal.pone.0240361
[5]
Wondemagegn, M., Bayeh, A., Mulat, Y., Tadesse, H., Haimanot, A. and Dereje, A. (2017) Rifampicin-Resistance Pattern of Mycobacterium tuberculosis and Associated Factors among Presumptive Tuberculosis Patients Referred to Debre Markos Referral Hospital, Ethiopia: A Cross-Sectional Study. BMC Research Notes, 10, Article No. 8. https://doi.org/10.1186/s13104-016-2328-4
[6]
Tembo Blackson, P.T. and Ntambwe, G.M. (2019) Prevalence and Factors Associated with Multidrug/Rifampicin Resistant Tuberculosis among Suspected Drug Resistant Tuberculosis Patients in Botswana. BMC Infectious Diseases, 19, Article No. 779. https://doi.org/10.1186/s12879-019-4375-7
[7]
Banu, S., Rhaman, M.T., Ahmed, S., Khatun, R., Ferdous, S.S., Hosen, B., Rahman, M.M., Ahmed, T., Cavanaugh, J.S. and Heffelfinger, J.D. (2017) Multidrug-Resistant Tuberculosis in Bangladesh: Results from a Sentinel Surveillance System. The International Journal of Tuberculosis and Lung Disease, 21, 12-17. https://doi.org/10.5588/ijtld.16.0384
[8]
Nhung, N.V., Hoa, N.B., Sy, D.N., Hennig, C.M. and Dean, A.S. (2015) The Fourth National Anti-Tuberculosis Drug Resistance Survey in Viet Nam. The International Journal of Tuberculosis and Lung Disease, 19, 670-675. https://doi.org/10.5588/ijtld.14.0785
[9]
Wasihun, A.G., Genet, G.H. and Dejene, T.A. (2021) Prevalence of Mycobacterium tuberculosis (Rifampicin-Resistant MTB) and Associated Risk Actors among Pulmonary Presumptive TB Patients in Eastern Amhara, Ethiopia: 2015-2019. Infectious Diseases and Therapy, 10, 1299-1308. https://doi.org/10.1007/s40121-020-00368-5
[10]
Hazir, R., Shakir, U.K., Muhamamd, A.K., Muhammad, Q., Abdul, J., Sadiq, N., Zakir, K., Mubbashir, H., Noor, M. and Nawab, A. (2017) Molecular Detection of Rifampicin Resistance by GeneXpert® Assay among Treated and Untreated Pulmonary Tuberculosis Patients from Khyber Pakhtunkhwa, Pakistan. Journal of Global Antimicrobial Resistance, 9, 118-120. https://doi.org/10.1016/j.jgar.2017.02.013
[11]
Steingart, K.R., Schiller, I., Horne, D.J., Pai, M., Boehme, C.C. and Dendukuri, N. (2014) Xpert® MTB/RIF Assay for Pulmonary Tuberculosis and Rifampicin Resistance in Adults. Cochrane Database of Systematic Reviews, No. 1, CD009593. https://doi.org/10.1002/14651858.CD009593.pub3
[12]
Farazi, A., Sofian, M., Zarrinfar, N., Katebi, F., Hoseini, S.D. and Keshavarz, R. (2013) Drug Resistance Pattern and Associated Risk Factors of Tuberculosis Patients in the Central Province of Iran. Caspian Journal of Internal Medicine, 4, 785-789.
[13]
Umair, M., Siddiqui, S.A. and Farooq, M.A. (2020) Diagnostic Accuracy of Sputum Microscopy in Comparison with GeneXpert in Pulmonary Tuberculosis. Cureus, 12, e11383. https://doi.org/10.7759/cureus.11383
[14]
Lebogang, K., Hannetjie, F., Faheem, S., Kennedy, O., Neil, A.M. and Ebrahim, V. (2020) Sputum Culture and Drug Sensitivity Testing Outcome among X-Pert Mycobacterium Tuberculosis/Rifampicin-Positive, Rifampicin-Resistant Sputum: A Retrospective Study—Not All Rifampicin Resistance Is Multi-Drug Resistant. Journal of Global Antimicrobial Resistance, 21, 434-438. https://doi.org/10.1016/j.jgar.2019.11.008
[15]
Kheira, G., Noria, H., Aissa, O., Soumia, Z., Feriel, S., Farid, B. and Sakina, C.T. (2016) Use of GeneXpert Mycobacterium tuberculosis/Rifampicin for Rapid Detection of Rifampicin Resistant Mycobacterium tuberculosis Strains of Clinically Suspected Multi-Drug Resistance Tuberculosis Cases. Annals of Translational Medicine, 4, Article 168. https://doi.org/10.21037/atm.2016.05.09