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Botanical Research 2023
水稻IQD基因家族分析及进化研究
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Abstract:
钙离子(Ca+)作为植物细胞中的一种信号分子，通过其下游的Ca+受体蛋白，即钙调素(CaM)与其靶蛋白结合，在环境信号反应和植物的生长发育中具有重要的作用。对于细胞内钙瞬变的刺激特异性产生、钙信号的解码和信号转化为细胞反应是转导过程的组成模块。IQD蛋白是一类在高等植物中特有的钙调蛋白结合蛋白，参与并调节钙调蛋白CaM (Calmodulin)与其他同源蛋白之间的相互作用。在这里，我们筛选了水稻中钙调素靶蛋白的比较基因组分析。本研究通过使用生物信息学软件对水稻IQD家族，进行结构分析、染色体位置、预测的蛋白质性质和基序、系统发育关系和进化史分析。在水稻基因组中，共鉴定出22个IQD家族基因。IQD家族基因可以分为3个亚组，位于一个亚组的IQD基因结构和基序组成相似。染色体定位分析，22个IQD家族基因分布在8条染色体上。进化树分析，发现水稻IQD家族基因与单子叶植物亲缘关系较近，与双子叶植物亲缘关系较远，在进化上出现物种间的差异性。GSE5属于IQD基因家族，酵母双杂交指出蛋白POW1与GSE5不存在互作关系，虽然这两种蛋白同时调控水稻籽粒大小，但是并没有通过互作方式在水稻籽粒中发挥作用，为深入研究IQD家族基因参与Ca+传导过程提供参考。
As a signaling molecule in plant cells, calcium ion (Ca+) binds to its target protein through its downstream Ca+ receptor protein, calmodulin (CaM), which plays an important role in environ-mental signaling response and plant growth and development. Stimulus-specific production of intracellular calcium transients, decoding calcium signaling, and signal conversion into cellular responses are the building blocks of the transduction process. IQD proteins are a class of calmo-dulin-binding proteins unique to higher plants, which participate in and regulate the interaction between calmodulin CaM (Calmodulin) and other homologous proteins. Here, we screen for comparative genomic analysis of calmodulin target proteins in rice. In this study, the structure analysis, chromosome location, predicted protein properties and motif of the rice IQD family, phylogenetic relationships, and evolutionary history were analyzed by using bioinformatics software. In the rice genome, a total of 22 IQD family genes were identified. IQD family genes can be divided into 3 subgroups, and IQD gene structure and motif composition are similar in one subgroup. Chromosome mapping analysis, 22 IQD family genes were distributed on 8 chromosomes. Evolutionary tree analysis showed that rice IQD family genes were closely related to monocots and distantly related to dicots, and there were evolutionary differences between species. GSE5 belongs to the IQD gene family, and yeast double hybridization points out that the protein POW1 and GSE5 do not interact with each other, although these two proteins regulate rice grain size at the same time, but do not play a role in rice grain through interaction. This paper provides a reference for in-depth study of the participation of IQD family genes in the Ca+ conduction process.
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