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Pharmacy Information 2023
基于网络药理学探讨蛇床子治疗排卵障碍型不孕症的作用机制
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Abstract:
目的：基于网络药理学研究蛇床子治疗排卵障碍型不孕症的作用机制。方法：从TCMSP、OMIM、Drugbank、GeneCards、PharmGkb数据库中确定蛇床子的潜在活性成分、作用靶点和疾病的差异基因，取两者交集，利用Cytoscape 3.9.0软件构建“中药–活性成分–交集靶点–疾病”调控网络图，string数据构建蛇床子和排卵障碍型不孕症的PPI网络；通过R包进行GO和KEGG功能富集分析。结果：经TCMSP数据库确定蛇床子有18个化合成分，59个药物靶标；经OMIM、Drugbank、GeneCards、PharmGkb数据库分析确定蛇床子治疗排卵障碍型不孕症靶点21个，利用string数据构建蛇床子和排卵障碍型不孕症的靶点。GO分析表明生物过程(BP)涉及类固醇激素反应、生殖腺发育、排卵周期等，细胞成分(CC)涉及孔隙复合体、薄膜筏、膜微区等区域，分子功能(MF)涉及半胱氨酸型内肽酶活性与凋亡过程、肾上腺素能受体活性、类固醇结合等生物学过程。KEGG结果表明，蛇床子治疗排卵障碍型不孕症的传导途径主要包括雌激素信号通路、细胞凋亡、VEGF信号通路、PI3K-Akt信号通路、IL-17信号通路等。结论：蛇床子改善排卵障碍型不孕症的作用机制可能是蛇床子中的beta-sitosterol、Stigmasterol、o-Isovaleryl columbianetin、O-Acetylcolumbianetin、Xanthoxylin N等有效成分通过影响AR、CASP3、PGR、PTGS2、ESR1等基因的表达进而调控了雌激素信号通路、细胞凋亡、VEGF信号通路、PI3K-Akt信号通路、IL-17信号通路等信号传导途径实现的。
Objective: To study the mechanism of ovulatory dysfunction infertility treated by Cnidium ossi-formis based on network pharmacology. Methods: From TCMSP, OMIM, Drugbank, GeneCards and PharmGkb databases, the potential active components, target sites and differential genes of the disease were determined, and the intersection of the two was selected. Cytoscape 3.9.0 software was used to construct the regulatory network diagram of “Traditional Chinese Medicine, active ingredient, intersection target-disease”, and PPI network of ovulatory dysfunction infertility was constructed with string data. Functional enrichment analysis of GO and KEGG was performed using R packets. Results: Eighteen compound components and 59 drug targets were determined by TCMSP database. Through the analysis of OMIM, Drugbank, GeneCards and PharmGkb databases, 21 targets for the treatment of ovulational dysfunction infertility were determined, and the targets for the treatment of ovulational dysfunction infertility and ovulational dysfunction infertility were constructed using string data. GO analysis showed that biological process (BP) involved steroid hormone response, gonad development, ovulation cycle, cellular component (CC) involved pore complex, membrane raft, membrane microregion and other regions, molecular function (MF) involved cysteine-type endopeptidase activity and apoptosis process, adrenergic receptor activity, steroid binding and other biological processes. KEGG results showed that the main pathways of cnidium os-sium in the treatment of ovulation disorder infertility include estrogen signaling pathway, apoptosis, VEGF signaling pathway, PI3K-Akt signaling pathway, IL-17 signaling pathway, etc. Conclusion: The mechanismof ambrosia cotyloides ameliorating ovulation disorder
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