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陶瓷膜表面固定化脂肪酶的研究
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Abstract:
本文利用(3-氨丙基)-三乙氧基硅烷(APTES)和甲基三甲氧基硅烷(MTMS)对片状陶瓷膜进行改性，再使用戊二醛(GA)交联得到陶瓷复合膜。最后，将脂肪酶固定在该陶瓷复合膜上，制备得到酶膜反应器。通过傅里叶变换红外光谱仪和X射线光电子能谱仪对样品进行表征。确定固定化的最佳条件为：APTES和MTMS浓度均为0.1 M、GA浓度为2%、固定化时间为3 h。固定化酶的最适操作pH值和温度分别为7、55℃。此外，固定化的酶具有较好的热稳定性和可重复使用性。
In this paper, ceramic planar membranes were modified with (3-aminopropyl)-triethoxysilane (APTES) and methyltrimethoxysilane (MTMS), and then cross-linked with the glutaraldehyde (GA) to obtain ceramic composite membranes. Finally, lipases were immobilized on these ceramic composite membranes to prepare the enzyme membrane reactor. The samples were characterized by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The optimal conditions for immobilization were determined as follows: APTES and MTMS concentrations of 0.1 M, GA concentration of 2%, and immobilization time of 3 h. The optimum operating pH and temperature of the immobilized enzyme were 7 and 55？C, respectively. In addition, the immobilized enzyme had better thermal stability and reusability.
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