|
|
螺卷毛壳菌Chaetomium cochliodes对玫瑰黑斑病病原菌的影响
|
Abstract:
为了筛选抗玫瑰黑斑病的内生真菌，本研究将一株来源于玫瑰茎部的内生真菌螺卷毛壳菌Chaetomium cochliodes作为拮抗菌与玫瑰黑斑病病原菌蔷薇盘二孢(Marssonina rosae)进行对峙培养，研究拮抗菌螺卷毛壳菌是否对玫瑰黑斑病病原菌蔷薇盘二孢具有抑制作用。研究结果表明，单独培养时，随着培养时间的延长，病原菌和拮抗菌菌落直径不断增加，不同培养时间菌落直径均具有显著性差异(P < 0.01)；拮抗培养时，拮抗菌随着时间的延长菌落直径仍不断增加，且不同培养时间菌落直径具有显著性差异(P < 0.01)，而病原菌在拮抗培养时菌落直径无显著性差异，拮抗菌对病原菌有显著的抑制效果，在培养72 h后抑制率达到60.7%，相对抑制率达到5.38，说明螺卷毛壳菌Chaetomium cochliodes对菌蔷薇盘二孢(Marssonina rosae)具有明显的抑制作用，可以作为防治玫瑰黑斑病的潜在生防菌。该研究结果为筛选抗玫瑰黑斑病潜在生防菌及开发利用提供理论依据。
The antagonistic effects of Chaetomium cochliodes, an endophytic fungus from the stem of Rosa ru-gosa and Marssonina rosae, the pathogen of Rose Black Spot, were studied by confrontation culture, in order to determine whether Chaetomium cochliodes could inhibit the growth of Marssonina rosae. The results showed that the diameter of Chaetomium cochliodes and Marssonina rosae increased with the culture time, and the diameter of them had significant difference at different culture time (P < 0.01) when they were cultured separately. When they were trained in confrontation, the colony diameter of Chaetomium cochliodes increased with time and had a significant difference at different culture time (P < 0.01), but there was no significant difference of Marssonina rosae. And the inhibi-tory rate of Chaetomium cochliodes to Marssonina rosae was up to 60.7% and the relative inhibitory rate was 5.38 after 72 hours, which indicated that Chaetomium cochliodes had significant inhibitory effect on Marssonina rosae, it can be used as a potential biocontrol agent to control rose black spot. The results provide a basis for screening potential biocontrol agents resistant to rose black spot.
| [1] | 和丽媛, 杨志龙. 玫瑰功能成分及产品开发研究进展[J]. 食品工业科技, 2020, 23(2): 1-15. |
| [2] | 陈真永, 吴鹏. 不同产地不同品种食用玫瑰抗氧化研究[J]. 西南农业学报, 2020, 33(10): 2215-2219. |
| [3] | 高建莉, 李罡. 食用玫瑰黑斑病的发生规律及防治技术[J]. 中国园艺文摘, 2017, 33(11): 183-184. |
| [4] | 田慧敏. 赤峰市区观赏植物防治及图鉴[M]. 赤峰: 内蒙古科学技术出版社, 2022. |
| [5] | 徐庭亮. 月季对蔷薇盘二孢(Marssonina rosae)入侵的防御机制研究[D]: [博士学位论文]. 北京: 北京林业大学, 2022. |
| [6] | 刘宝军. 月季黑斑病菌蔷薇盘二孢(Marssonina Rosae)形态多样性、致病性多样性和遗传多样性研究[D]: [硕士学位论文]. 泰安: 山东农业大学, 2010. |
| [7] | 吕博, 孟庆忠, 张成, 等. 微生物菌肥对棉花黄萎病的防治效果研究[J]. 农村经济与科技, 2020, 31(23): 64-65. |
| [8] | Sapna, S. (2014) Protein Mapping of Chaetomium globosum, Apotential Biological Control Agent through Proteomics Approach. Journal of Plant Biochemistry and Biotechnology, 23, 284-292. https://doi.org/10.1007/s13562-013-0213-y |
| [9] | 付雯, 张晓勇. 毛壳菌生物防治机制研究进展[J]. 中国农学通报, 2012, 28(7): 100-103. |
| [10] | 廖宏娟, 张志斌, 江玉梅, 等. 球毛壳菌对植物病原真菌和根结线虫的生物防治潜力[J]. 天然产物研究与开发, 2022, 34: 1076-1089. |
| [11] | 李艳, 赵丽红, 张亚林, 等. 球毛壳菌CEF-082对棉花的防病促生作用[J]. 中国棉花, 2022, 49(5): 9-13. |
| [12] | Waksman, S.A. and Bugie, E. (1944) Chaetomin, a New Anti-biotic Substance Produced by Chaetomium cochliodes: I. Formation and Properties. Journal of Bacteriology, 48, 527-530. https://doi.org/10.1128/jb.48.5.527-530.1944 |
| [13] | Wang, M.H., Zhang, X.Y., Tan, X.M., et al. (2020) Cheto-cochliodins A-I, Epipoly(thiodioxopiperazines) from Chaetomium cochliodes. Journal of Natural Products, 83, 805-813. https://doi.org/10.1021/acs.jnatprod.9b00239 |
| [14] | Timothy, R.W. and Robert, M.W. (2013) Studies on the Bio-synthesis of Chetomin: Enantiospecific Synthesis of a Putative, Late-Stage Biosynthetic Intermediate. Tetrahedron, 69, 770-773. https://doi.org/10.1016/j.tet.2012.10.075 |
| [15] | 杨恩超, 杨涛, 李宗珍, 等. 螺卷毛壳霉代谢产物的抗菌活性研究[J]. 天然产物研究与开发, 2008, 20(6): 953-955, 963. |
| [16] | Safe, S. and Taylor, A. (1972) Sporidesmins. 8. Ovine Ill-Thrift in Nova Scotia. 3. The Characterisation of Chetomin a Toxic Metabolite of Chaetomium cochliodes and Chaetomium globosum. Journal of the Chemical Society, Perkin Transactions 1, 4, 472-479. https://doi.org/10.1039/p19720000472 |
| [17] | 周秀华, 崔磊. 螺卷毛壳对樟子松枯梢病病原菌的影响[J]. 长春大学学报, 2009, 19(10): 69-70, 74. |
