Our aim was to evaluate the quality of
ejaculated and epididymal frozen-thawed pig sperm of endangered Vietnam native pig breeds.
Ejaculated sperm was collected from live boars and epididymal sperm was
collected from slaughtered boars of the MuongTe, Kieng Sat and Co BinhThuanbreeds and frozen in 0.25ml straws using a protocol established earlier for
modern pig breeds. We evaluated the sperm quality after thawing in terms of
motility and rates of viable and abnormal spermatozoa. Our results revealed
that the sperm motility and rates of viable and
abnormal frozen-thawed sperm were >30%, >44%, and <14%, respectively. The origin of sperm had an
effect on the production of pig embryos in
vitro.In the Co BinhThuan breed, ejaculated sperm generated higher
cleavage, blastocyst and hatching rates than did the epididymal sperm
(60.11% vs56.02%, 17.23% vs 14.31%, 3.78% vs 2.34%,
respectively, P < 0.05). Although no difference in cleavage rate, blastocyst
formation rate and the average number of cells/blastocysts, the hatching blastocyst rate was different between the breeds (P > 0.05). In the Co BinhThuan
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