Introduction: Tuberculosis is caused by infection with Mycobacterium tuberculosis. Looking at
the evolution of the bacterium gene due to mutation is crucial to identify
speciescirculating among patients in
an area. WHO speculated that tuberculosis is caused by M. tuberculosis (MTB),
but identification of the strains of MTB circulating in a particular area is
important for the management of MTB and to identify pulmonary infections caused
by non-tuberculosis mycobacterium. Contact tracing of drug resistant MTB in
circulation in an area is also an important procedure of MTB therapeutic
choice. Aim: This study aimed to isolate and identify Mycobacterium speciescirculating in Bayelsa State, Nigeria. Materials
and Methods: A total of 102 sputum samples collected from MTB patients were
cultured in Lowenstein Jensen (LJ) solid media. Isolates on LJ media were
confirmed using Zeihl Nelseen staining method for AFB and Standard Diagnosis
Bioline TB Ag MPT64 Rapid test kit. The 16s rRNA gene amplification, agarose
gel electrophoresis, and gene sequencing were conducted. Phylogenic analysis
and evolutional distances of the strains are computed using the Juke-cantor
method. Result: Out of 102 sputum samples examined 15(14.7%)
had growth of Mycobacterium species
(AFB positive). The extracted DNA of MTB amplified on agarose gel
electrophoresis aligned horizontally at
References
[1]
Barberies, N.L., Bragazzi, L., Galluzzo, F. and Martaini, M. (2017) The History of Tuberculosis: From the Historical Records to the Isolation of Koch’s Bacillus. Journal of Preventive Medicine and Hygiene, 58, 9-12.
[2]
Luca, S., Morris, M. D., Quezada, L., Bhat, P., Moser, K., Smith, J., Perez, H. and Mihaescu, T. (2013) History of BCG Vaccine. Medical, 8, 53-58.
[3]
Gagneux, S. (2012) Host-Pathogen Coevolution in Human Tuberculosis. Philosophical Transaction of the Royal Society of London Biological Science, 367, 850-859. https://doi.org/10.1098/rstb.2011.0316
[4]
Guerra-Assunção, J.A., Crampin, A.C., Houben, R.M.G.J., Mzembe, T., Mallard, K., Coll, F., Khan, P., Banda, L., Chiwaya, A., Pereira, R.P.A., McNerney, R., Fine, P.E.M., Parkhill, J., Clark, T.G. and Glynn, J.R. (2015) Large-Scale Whole Genome Sequencing of Mycobacterium tuberculosis Provides Insights into Transmission in a High Prevalence Area. eLife, 4, e05166. https://doi.org/10.7554/eLife.05166
[5]
Srinand, S.P., Kathryn, E.S., Nancy, D.C., Barry, N.K., Thomas, S.W. and James, M.M. (1997) Restricted Structural Gene Polymorphism in the Mycobacterium tuberculosis Complex Indicates Evolutionarily Recent Global Dissemination. Proceedings of the National Academic of Sciences of the United States of America, 94, 9869-9874. https://doi.org/10.1073/pnas.94.18.9869
[6]
Feyzioglu, B., Dogan, M., Sanli, O.O., Ozdemir, M. and Baykan, M. (2014) Comparison of the Performance of TK System with LJ and MGIT Methods in the Diagnosis of Tuberculosis. International Journal of Clinical Experimental Medicine, 7, 1084-1088.
[7]
Audu, E.S., Gambo, M.S. and Yakubu, A.A. (2017) Rifampicin Resistant Mycobacterium tuberculosis in Nasarawa State, Nigeria. Nigerian Journal of Basic Clinical Sciences, 14, 21-25. https://doi.org/10.4103/0331-8540.204075
[8]
Global Laboratory Initiative (2014) Mycobacteriology Laboratory Manual Processing Sputum for Smear Microscopy and Qualitative Culture. First Edition, Otsuka Pharmaceutical Co, Ltd., Otsuka, Japan, 19-50.
[9]
Saitou, N. and Nei, M. (1987) The Neighbor-Joining Method: A New Method for Reconstructing Phylogenetic Trees. Molecular Biology and Evolution, 4, 406-425.
[10]
Mbugi, E.V., Katale, B.Z., Siame, K.K., Keyyu, J.D., Kendall, S.L., Dockrell, H.M. et al. (2015) Genetic Diversity of Mycobacterium tuberculosis Isolated from Tuberculosis Patients in the Serengeti Ecosystem in Tanzania. Tuberculosis, 95, 170-178. https://doi.org/10.1016/j.tube.2014.11.006
[11]
Ani, A., Bruvik, T., Okoh, Y., Agaba, P., Agbaji, O., Idoko, J. and Dahle, U.R. (2010) Genetic Diversity of Mycobacterium tuberculosis Complex in Jos, Nigeria. BMC Infectious Diseases, 10, Article No. 189. https://doi.org/10.1186/1471-2334-10-189
[12]
Lawson, L., Zhang, J., Gomgnimbou, M.K., Abdurrahman, S.T., Le Moullec, S., Mohamed, F., et al. (2012) A Molecular Epidemiological and Genetic Diversity Study of Tuberculosis in Ibadan, Nnewi and Abuja, Nigeria. PLOS ONE, 76, e38409. https://doi.org/10.1371/journal.pone.0038409
[13]
Molina-Moya, B., Abdurrahman, S.T., Madukaji, L., Gomgnimbou, M.K., Spinasse, L., Gomes-Fernandes, M., Gomes, H.M., Sarah, K., Russell, D., Bimba, J.S., Lovett, L., Christophe, S., Luis, E.C. and Dominguez, J. (2018) Genetic Characterization of Mycobacterium Tuberculosis Complex Isolates Circulating in Abuja, Nigeria. Infection and Drug Resistance, 11, 1617-1625.
[14]
Jenkins, A.O., Cadmus, S.I., Venter, E.H., Pourcel, C. and Hauk, Y. (2011) Molecular Epidemiology of Human and Animal Tuberculosis in Ibadan, Southwestern Nigeria. Veterinary Microbiology, 151, 139-147. https://doi.org/10.1016/j.vetmic.2011.02.037
[15]
Charm, J. and Biswas, T. (2013) How to Calculate Sample Size for Different Study Designs in Medical Research. Indian Journal of Psychological Medicine, 35, 121-126. https://doi.org/10.4103/0253-7176.116232
[16]
Felsenstein, J. (1985) Confidence Limits on Phylogenies: An Approach Using the Bootstrap. Evolution, 39, 783-791. https://doi.org/10.2307/2408678
[17]
Jukes, T.H. and Cantor C.R. (1969) Evolution of Protein Molecules. In: Munro, H.N., Ed., Mammalian Protein Metabolism, Academic Press, New York, 21-132. https://doi.org/10.1016/B978-1-4832-3211-9.50009-7
[18]
Song, J.Y., Sohn, J.W., Jeong, H.W., Cheong, H.J., Kim, W.J. and Kim, M.J. (2006) An Outbreak of Post-Actipuncture Cutaneous Infection Due to Mycobacterium abscessus. BMC Infectious Diseases, 6, 118-120. https://doi.org/10.1186/1471-2334-6-6
[19]
John, L.J. and Jerrold, J.E. (2006) Tuberculosis and Atypica Mycobacterial Infection. Second Edition, Churchill Livingstone, London, 1-65.
[20]
Ani1, A.T.B., Yetunde, O.P.A., Oche, A., John, I. and Ulf, R.D. (2010) Genetic Diversity of Mycobacterium tuberculosis Complex in Jos, Nigeria. Biomedical Central of Infectious Diseases, 10, Article No. 189. https://doi.org/10.1186/1471-2334-10-189
[21]
Lawson, L., Zhang, J., Gomgnimbou, M.K., Abdurrahman, S.T. and Le Moullec, S. (2012) A Molecular Epidemiological and Genetic Diversity Study of Tuberculosis in Ibadan, Nnewi and Abuja, Nigeria. Nigeria. Public Library of Science, 7, e38409. https://doi.org/10.1371/journal.pone.0038409
[22]
Gashaw, F., Berhanu, E., Yalemtsehay, M., Bazezew, Y., Misikir, A., Balako, G. and Gobena, A. (2021) Phenotypic and Genotypic Drug Sensitivity Profiles of Mycobacterium tuberculosis Infection and Associated Factors. BMC Infectious Diseases, 21, Article No. 261. https://doi.org/10.1186/s12879-021-05961-8
[23]
Senghore, M., Out, J., Witney, A., Gehre, F., Doughty, E.L. and Kay, G.L. (2017) Whole-Genome Sequencing Illuminates the Evolution and Spread of Multidrug-Resistant Tuberculosis in Southwest Nigeria. Public Library of Science, 12, 184-510. https://doi.org/10.1371/journal.pone.0184510
[24]
Li, Y., Xinrui, C., Shiming, L., Hao, W., Jianlin, W., Peng, L., Jing, W., Zhi, Z., Huixia, G., Machao, L., Kanglin, W. and Erhei, D. (2016) Characterization of Mycobacterium tuberculosis Isolates from Hebei, China, Genotypes and Drug Susceptibility Phenotypes. BMC Infectious Diseases, 16, Article No. 107. https://doi.org/10.1186/s12879-016-1441-2
