This article presents data on the introduction of in
vitro culture and microclonal propagation of plants identified in the group
of hyperhalophytes belonging to the species Suaeda arcuataBunge. This study was carried
out to optimize the composition of nutrient media for the main stages of
reproduction in vitro, as well as studies on the rooting and adaptation
of regenerants for species of the genus Suaeda obtained from axillary or
apical buds, but more often from stem segments with a node. In this work, hormones
of the cytokinin and auxin series, or a combination of them, were added to the
nutrient environment for growth activation. The analyzing of microplants for
the content of soluble in water B vitamins was carried out. As a result of the
research, it was found that intact Suaeda
arcuata plants in their natural habitats produce a large amount of
soluble in water vitamins: riboflavin—0.062% and thiamine up to 0.006%. And in
regenerated plants obtained on media without hormones, the content of vitamins
was: B2 0.053%, B1 0%, respectively, and with a combination of 1/2 MS + 1 mg/l
6-BAP + 0.3 mg/l IAA + 2, 4-D, the content of vitamins varied as follows:
riboflavin—0.059%, folic acid—0.030%, and thiamine was not detected. The
cultivation of regenerates on the environment 1/2 MS + 1 mg/l 6-BAP + 0.3 mg/l
IAA + 2,4-D showed the best effect on the growth of regenerants, created the
possibility of obtaining the maximum amount of biomass and accumulation of B
vitamins.
References
[1]
Breckle, S.-W. (2021) An Ecological Overview of Halophytes from the Aralkum Area. In: Grigore, M.-N., Ed., Handbook of Halophytes, Springer, Cham, 393-449.
https://doi.org/10.1007/978-3-030-57635-6_15
[2]
Khalbekova, K. (2023) Polyphenols of the Hyperhalophyte Halocnemum strobilaceum (Pall.) M. Bieb. and Antioxidant Activity. American Journal of Plant Sciences, 14, 653-661. https://doi.org/10.4236/ajps.2023.146044
[3]
Miftakhova, A.F., Burasheva, G.S. and Abilov, Z.A. (1999) Flavonoids of Halocnemum strobilaceum. Chemistry of Natural Compounds, 35, 100-101.
https://doi.org/10.1007/BF02238224
[4]
Akzhigitova, N.I. (1982) Halophilic Vegetation of Central Asia and Its Indicator Properties. Fan, Tashkent, 192 p.
[5]
Shomurodov, H.F., Rakhimova, T.T., Saribaeva, S.U., Rakhimova, N.K., Esov, R.A., Adilov, B.A. (2013) Perspective Plant Species for Stabilization of Sand Dunes on the Exposed Aral Sea Bed. Journal of Earth Science and Engineering, 3, 655-662.
[6]
Mohammed, H.A., Emwas, A.H. and Khan, R.A. (2023) Salt-Tolerant Plants, Halophytes, as Renewable Natural Resources for Cancer Prevention and Treatment: Roles of Phenolics and Flavonoids in Immunomodulation and Suppression of Oxidative Stress towards Cancer Management. International Journal of Molecular Sciences, 24, Article No. 5171. https://doi.org/10.3390/ijms24065171
[7]
Ovcharov, K.E. (1964) Plant Vitamins. 15-30.
[8]
Butenko, R.G. (1999) Biology of Cells of Higher Plants in Vitro and Biotechnology Based on Them. 20-50.
[9]
Adylov, T.A. (1970) Poisonous and Alkaloid-Bearing Plants of Uzbekistan. Fan, Tashkent, 48-49.
[10]
(2004) Control Methods. Chemical Factors. Guidance on Methods of Quality Control and Safety of Biologically Active Food Additives. Guidance R 4.1.1672-03. Federal Center for State Sanitary and Epidemiological Surveillance of the Ministry of Health of Russia, 30-45.
[11]
Bochantsev, V.P. (1953) Family Chenopodiaceae. In: Flora of Uzbekistan, Vol. 6, Academy of Sciences of the Uzbek SSR, Tashkent, 265-290.
[12]
Costa-Becheleni, F.R., Troyo-Diéguez, E., Nieto-Garibay, A., Bustamante-Salazar, L.A., García-Galindo, H.S. and Murillo-Amador, B. (2022) Interruption of Seed Dormancy and in Vitro Germination of the Halophile Emerging Crop Suaeda edulis (Chenopodiaceae). Agronomy, 12, Article No. 103.
https://doi.org/10.3390/agronomy12010103
[13]
Singh, A., Chikara, J. and Pandya, J.B. (2004) Plant Regeneration from Callus Cultures in Suaeda nudiflora (Wild.) Moq. Indian Journal of Biotechnology, 3, 454-456.
[14]
Joshi, A., Kanthaliya, B. and Arora, J. (2019) Evaluation of Growth and Antioxidant Activity in Suaeda monoica and Suaeda nudiflora Callus Cultures under Sequential Exposure to Saline Conditions. Current Biotechnology, 8, 42-52.
https://doi.org/10.2174/2211550108666190507122304
[15]
Murashige, T. and Skoog, F. (1962) A Revised Medium for Rapid Growth and Bio-Assays with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 473-497.
https://doi.org/10.1111/j.1399-3054.1962.tb08052.x