While
generally recognized as a potential source of contamination during the
collection process, lubricants are often used at the preference of the male
partner to prevent irritation. While older lubricants have been studied, there
is currently no conscience within programs as to what constitutes a “safe”
lubricant. The object of the current study was designed to evaluate chemically
unique “next generation” lubricants in comparison to lubricants currently in
use in fertility treatment and/or recognized as fertility safe; the first was a
silicone-based lubricant, the second is a
water-based, plant-based organic compound, in comparison to two
established medical lubricants and a control. Twelve deidentified semen samples
from the clinical andrology laboratory were used to test the lubricants
following semen analysis. In order to enter the study, the sample had to have a
minimum of 30 × 106 motile cells. Samples were then processed using
a simple sperm wash modified to reconstitute the pellet into a final volume of
7 mL. Half-milliliter aliquots were then transferred into 13 wells of a
standard 24-well culture plate. One well was used as a control. The remaining
wells received one of the four lubricants at one of three volumes (10, 50, or
100 uL), producing 12 treatment combinations (four lubricants + 3 concentration
levels) and the control. The samples were then cultured at room temperature for
24 hours. At times 0, 1, 3, 12, and 24 hrs, the plate was agitated to remix the
sample, and a 4 uL aliquot of each well was analyzed for standard semen
parameters using a computer-assisted semen analyzer. Results indicated the
expected decrease in semen parameters over time in all treatments (P <
0.001). There was also a dose-dependent drop in most of the lubricants.
However, samples contaminated with the newer lubricants appeared to maintain
semen parameters similar to the controls at all but the 100 ul level of
contamination, while the older lubricants caused decreases in sperm function at
much lower concentrations. While semen parameters alone should not be the only
criteria for the selection of a lubricant, the present study suggests newer
formulations of lubricant are less likely to interfere with sperm function.
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