For the
production of a reference material from caffeine solution, one of the methods
of characterization was HPLC-UV since caffeine is very sensitive to the UV. In
this work, a batch solution of caffeine in water reference material of 1000
mg/kg has been gravimetrically prepared using a calibrated analytical balance.
A sample of this solution was diluted to 25mg/kg for measurement by HPLC-UV in the range 10-50 mg/kg. The chromatographic separation was carried
out by C-18 column and a mobile phase assembled of 75% water and 25% methanol (v:v). The detection was made by
the UV detector at 275 nm. The validation of this analytical method was carried
out in accordance with requirements of the EURACHEM and ICH guidelines. The
selectivity, linearity, accuracy, precision and trueness (recovery and bias) of
the method were studied. The validation results proved that the method is
fit-for-purpose of measuring the caffeine concentration in water in the range
10-50 mg/kg using HPLC-UV.
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