LC-MS Phytochemical Profiles of Phenolic Compounds and Antimicrobial, Antioxidant, and Antiplasmodial Activities of Ethanol Extracts of Pycnostachys erici-rosenii R.E.Fr and Leucas martinicensis (Jack.) R.Br (Lamiaceae)
This study has been conducted to analyze the chemical composition and antimicrobial, antioxidant and antiplasmodial properties of ethanol extracts of Pycnostachys erici-rosenii R.E.Fr and Leucas martinicensis (Jack.) R.Br that are not yet well known. The HPLC-DAD-MS-ESI+ method was used to investigate the chemical profile, the disc diffusion and microdilution method for the antimicrobial activities, the DPPH and FRAP assays for antioxidant property and SYBR Green I-based growth inhibition assay on Pf 3D7 (CQ-sensitive) strain for antiplasmodial properties. The results indicated significant amounts of hydroxybenzoic acid, flavonol and hydroxycinnamic acid in the two species. The total polyphenols analysis gave 209.0 ± 16.1 mg GAE/100g DW for P. erici-rosenii and 175.3 ± 1.6 mg GAE/100g DW for L. martinicensis while for total flavonoids analysis 33.4 ± 1.8 mg TE/100g DW was found for P. erici-rosenii and 39.6 ± 0.6 mg TE/100g DW for L. martinicensis. Moreover, antimicrobial tests revealed 0.125 mg/mL on E. faecalis ATCC25922 for ethanol extracts P. erici-rosenii and L. martinicensis. The antioxidant assays revealed IC50 56.17 μg/mL and 78.43 μg/mL for DPPH assay, and 112.03 μM TE/100mL and 90.46 μM TE/100mL) for FRAP assay for ethanol extracts of P. erici-rosenii and L. martinicensis respectively. Finally, for Antiplasmodial activity, IC50 was 4.07 μg/mL for P. erici-rosenii and 12.29 μg/mL for L. martinicensis. Therefore, the antimicrobial, the antioxidant, and the antiplasmodial results suggest that the ethanolic extracts of the aerial parts of P. erici-rosenii and L. martinicensis contain compounds with antimicrobial, antioxidant, and antiplasmodial properties, which can be searched in new drug discovery for treatment of infectious diseases.
References
[1]
Naghibi, F., Mosaddegh, M., Mohammadi, M.S. and Ghorbani, A. (2005) Labiatae Family in Folk Medicine in Iran. Iranian Journal of Pharmaceutical Research, 2, 63-79.
[2]
Noura, S.D. and William, N.S. (2018) The Genus Conradina (Lamiaceae): A Review. Plants, 7, Article 19. https://doi.org/10.3390/plants7010019
[3]
O’Neill, J. (2016) Antimicrobial Resistance: Tackling a Crisis for the Health and Wealth of Nations. Review on Antimicrobial Resistance. https://amr-review.org/
[4]
De Kraker, M.E.A., Stewardson, A.J. and Harbarth, S. (2016) Will 10 Million People Die a Year Due to Antimicrobial Resistance by 2050? PLOS Medicine, 13, e1002184. https://doi.org/10.1371/journal.pmed.1002184
[5]
WHO: World Health Organization (2022) World Malaria Report 2022. Geneva.
[6]
Farah, H., Elbadrawy, E. and Al-Atoom, A.A. (2015) Evaluation of Antioxidant and Antimicrobial Activities of Ethanolic Extracts of Parsley (Petroselinum erispum) and Coriander (Coriandrum sativum) Plants Grown in Saudi Arabia. International Journal of Advanced Research, 3, 1244-1255.
[7]
Catunescu, G.M., Rotar, I., Vidican, R., Bunghez, F. and Rotar, A.M. (2017) γ Radiation Enhances the Bioactivity of Fresh Parsley (Petroselinum crispum (Mill.) Fuss Var. Neapolitanum). Radiation Physics and Chemistry, 132, 22-29. https://doi.org/10.1016/j.radphyschem.2016.11.010
[8]
Dumitras, D.A., Bunea, A., Vodnar, D.C., Hanganu, D., Pall, E., Cenariu, M., Gal, A.F. and Andrei, S. (2022) Phytochemical Characterization of Taxus baccata L. Aril with Emphasis on Evaluation of the Antiproliferative and Pro-Apoptotic Activity of Rhodoxanthin. Antioxidants, 11, Article 1039. https://doi.org/10.3390/antiox11061039
[9]
Mihai, B., Andrei, M., Laurian, V., Ovidiu, C., Irina, I., Ana-Maria, G., Dan-Cristian, V., Gianina, C. and Ramona P. (2018) Phytochemical Analysis, Antioxidant and Antimicrobial Activities of Helichrysum arenarium (L.) Moench. and Antennaria dioica (L.) Gaertn. Flowers. Molecules, 23, Article 409. https://doi.org/10.3390/molecules23020409
[10]
Benedec, D., Hanganu, D., Lorena, F., Oniga, I., Brindusa, T., Olah, N.K., Gheldiu, A.M., Raita, O. and Vlase, L. (2017) Chemical, Antioxidant and Antibacterial Studies of Romanian Heracleum sphondylium. Farmacia, 65, 252-256. https://farmaciajournal.com/arhiva/201702/art-15-Benedec_Oniga_Vlase_252-256.pdf
[11]
Mot, A., Damian, G., Sarbu, C. and Silaghi-Dumitrescu, R. (2009) Redox Reactivity in Propolis: Direct Detection of Free Radicals in Basic Medium and Interaction with Hemoglobin. Redox Report, 14, 267-274. https://doi.org/10.1179/135100009X12525712409814
[12]
Arendrup, M.C., Hope, W., Cuenca-Estrella, M. and Lass-Flörl, C. (2012) EUCAST Technical Note on the EUCAST Definitive Document EDef 7.2: Method for the Determination of Broth Dilution Minimum Inhibitory Concentrations of Antifungal Agents for Yeasts EDef 7.2 (EUCAST-AFST). Clinical Microbiology and Infection, 18, 246-247. https://doi.org/10.1111/j.1469-0691.2012.03880.x
[13]
Danciu, C., Muntean, D., Alexa, E., Watz, C., Oprean, C., Zupkó, I., Bor, A., Minda, D., Proks, M. and Buda, V. (2018) Phytochemical Characterization and Evaluation of the Antimicrobial, Antiproliferative and Pro-Apoptotic Potential of Ephedra alata Decne. Hydroalcoholic Extract against the MCF-7 Breast Cancer Cell Line. Molecules, 24, Article 13. https://doi.org/10.3390/molecules24010013
[14]
Muntean, D., Licker, M., Alexa, E., Popescu, I., Jianu, C., Buda, V., Dehelean, C.A., Ghiulai, R., Horhat, F.G. and Horhat, D. (2019) Evaluation of Essential Oil Obtained from Mentha piperita L. against Multidrug-Resistant Strains. Infection and Drug Resistance, 12, 2905-2914. https://doi.org/10.2147/IDR.S218141
[15]
Trager, W. and Jensen J.B. (1976) Human Malaria Parasites in Continuous Culture. Science, 193, 673-675. https://doi.org/10.1126/science.781840
[16]
Lambros, C. and Vanderberg, J.P. (1979) Synchronization of Plasmodium Falciparum Erythrocytic Stages in Culture. The Journal of Parasitology, 65, 418-432. https://doi.org/10.2307/3280287
[17]
Smilkstein, M., Sriwilaijaroen, N., Kelly, J.X., Wilairat, P. and Riscoe, M. (2004). Simple and Inexpensive Fluorescence-Based Technique for High-Throughput Antimalarial Drug Screening. Antimicrobial Agents and Chemotherapy, 48, 1803-1806. https://doi.org/10.1128/AAC.48.5.1803-1806.2004
[18]
Singh, N., Kaushik, N.K., Mohanakrishnan, D., Tiwari, S.K. and Sahal, D. (2015) Antiplasmodial Activity of Medicinal Plants from Chhotanagpur Plateau, Jharkhand, Indian. Journal of Ethnopharmacology, 165, 152-162. https://doi.org/10.1016/j.jep.2015.02.038
[19]
Bădălica-Petrescu, M., Drăgan, S., Ranga, F., Fetea, F. and Socaciu, C. (2014) Comparative HPLC-DAD-MS-ESI+ Fingerprint and Quantification of Phenolic and Flavonoid Composition of Aqueous Leaf Extracts of Cornus mas and Crataegus monogyna, in Relation to Their Cardiotonic Potential. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 42, 9-18. https://doi.org/10.15835/nbha4219270
[20]
Hanganu, D., Niculae, M., Ielciu, I., Olah, N.K., Munteanu, M., Burtescu, R.S., Tefan, R., Olar, L., Pall, E. and Andrei, S., (2021) Chemical Profile, Cytotoxic Activity and Oxidative Stress Reduction of Different Syringa vulgaris L. Extracts. Molecules, 26, Article 3104. https://doi.org/10.3390/molecules26113104
[21]
Boizot, N. and Charpentier, J.P. (2006) Méthode rapide d’évaluation du contenu en composés phénoliques des organes d’un arbre forestier. https://www.researchgate.net/profile/Nathalie-Boizot/publication/341819016_Methode_rapide_d'evaluation_du_contenu_en_composes_phenoliques_des_organes_d'un_arbre_forestier/links/60d1f22da6fdcce58ba7f0e3/Methode-rapide-devaluation-du-contenu-en-composes-phenoliques-des-organes-dun-arbre-forestier.pdf
[22]
Di Carlo, G., Mascolo, N., Izzo, A.A. and Capasso, F. (1999) Flavonoids: Old and New Aspects of a Class of Natural Therapeutic Drugs. Life Sciences, 65, 337-353. https://doi.org/10.1016/S0024-3205(99)00120-4
[23]
Nakujima, T., Manishi, M.I., Yamamoto, K., Cyong, J.C. and Hirai, K. (2001) Inhibitory Effects of Baicalein, a Flavonoid in Scutellaria Root, on Eotaxin Production by Human Dermal Fibroblasts. Planta Medica, 67, 132-135. https://doi.org/10.1055/s-2001-11532
[24]
Eibi, G. and Wagner, H. (1991) A New Method for the in vivo Screening of Inhibitors of Angiotensin Converting Enzyme (ACE), Using the Chromophore Labeled Substrate Dansyltriglycine. Planta Medica, 57, 137-141. https://doi.org/10.1055/s-2006-960050
Shahat, A.A., Cos, P., Bruyne, T.D., Apers, S., Hammouda, F.M., Ismail, S.I., Azzam, S., Claeys, M., Goovaerts, E., Pieters, L., Berghe, D.V. and Vlietinck, A.J. (2002) Antiviral and Antioxidant activity of Flavonoids and Proanthocyanidins from Crataegus sinaica. Planta Medica, 68, 539-541. https://doi.org/10.1055/s-2002-32547
[27]
Colette, N., Bernier, J.L., Henichart, J.P., Catteau, J.P., Gaydou, E.M. and Wallt, J.C. (1992) Scavanger and Antioxydant Properties of Ten Synthetic Flavones. Free Radical Biology and Medicine, 13, 211-219. https://doi.org/10.1016/0891-5849(92)90017-B
[28]
Murakami, N., Mostaqul, H.M., Tamura, S., Itagak, S. and Horii, T. (2001) A New Anti-Malarial Flavonol Glycoside from Hydrangeae dulcis Folium (Hydrangea macrophylla). Bioorganic and Medicinal Chemistry Letters, 11, 2445-2447. https://doi.org/10.1016/S0960-894X(01)00467-X
[29]
Barreira, J., Ferreira, I., Oliveira, M. and Pereira, J. (2008) Antioxidant Activities of the Extracts from Chestnut Flower, Leaf, Skins and Fruit. Food Chemistry, 107, 1106-1113. https://doi.org/10.1016/j.foodchem.2007.09.030
[30]
Lamaison, J.L. and Freytet, P.J.C. (1996) Medicinal Lamiaceae with Antioxidant Activity, Potential Sources of Rosmarinic Acid. Pharmaceutica Acta Helvetiae, 66, 185-188.
[31]
Zheng, W. and Wang, S.Y. (2001) Antioxidant Activity and Phenolic Compound in Selected Herbs. Journal of Agricultural and Food Chemistry, 49, 5165-5170. https://doi.org/10.1021/jf010697n
[32]
Shan, B., Cai, Y.Z., Sun, M. and Corke, H. (2005) Antioxidant Capacity of 26 Spice Extracts and Characterization of Their Phenolic Constituents. Journal of Agricultural and Food Chemistry, 53, 7749-7759. https://doi.org/10.1021/jf051513y
[33]
Kuete, V., Nana, F., Ngameni, B., Mbaveng, A.T., Keumedjio, F. and Nyadjui, B.T. (2009) Antimicrobial Activity of the Crude Extract, Fractions and Compounds from Stem of Ficus ovata (Moraceae). Journal of Ethnopharmacology, 124, 556-561. https://doi.org/10.1016/j.jep.2009.05.003
[34]
Sarac, N. and Ugur, A. (2007) Antimicrobial Activities and Usage in Folkloric Medicine of Some Lamiaceae Species Growing in Mugla, Turkey. EurAsian Journal of BioSciences, 4, 28-37. http://www.ejobios.com/content/1/4/28-34
[35]
Murugan, K., Aarthi, N. and Kovendan, K. (2015) Mosquitocidal and Antiplasmodial Activity of Senna occidentalis (Cassiae) and Ocimum basilicum (Lamiaceae) from Maruthamalai Hills against Anopheles stephensi and Plasmodium falciparum. Parasitology Research, 114, 3657-3664. https://doi.org/10.1007/s00436-015-4593-x
[36]
Tona, L., Cimanga, R.K., Mesia, K., Musuamba, C.T., De Bruyne, T., Apers, S., Hernans, N., Miert, S.V., Pieters, L., Totté, J. and Vlietinck, A.J. (2004) In vitro Antiplasmodial Activity of Extracts and Fractions from Seven Medicinal Plants Used in Democratic Republic of Congo. Journal of Ethnopharmacology, 93, 27-32. https://doi.org/10.1016/j.jep.2004.02.022
[37]
Maria, C.S., Marika, P., Manuel, S., Paola, P., Antonella, R. and Dario, C. (2018) Analysis of Polyphenols in the Lamiaceae Family by Matrix Solid-Phase Dispersion Extraction Followed by Ultra-High-Performance Liquid Chromatography—Tandem Mass Spectrometry Determination. ACS Omega, 3, 17610-17616. https://doi.org/10.1021/acsomega.8b02239