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Botanical Research 2024
鱼腥草中HtbZIP60基因的克隆和生物信息分析
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Abstract:
bZIP是植物抗逆性领域关键的转录因子，能调控细胞生物学中的各种生命过程，包括生长、发育、应激响应和代谢调控。为了深入研究鱼腥草中bZIP60基因的功能与调控机制，以鱼腥草为材料提取总RNA，合成cDNA第一链，然后成功克隆出鱼腥草HtbZIP60基因，并对其进行相关的生物信息学分析。结果表明，HtbZIP60的编码序列全长为996 bp，编码331个氨基酸，蛋白质分子质量37447.00 ku，不含信号肽剪切位点，包含跨膜结构域，以α螺旋为主要构成，定位于细胞核，具有30个已知功能的启动子。这些发现为深入研究HtbZIP60在鱼腥草生长与发育中的生物学功能提供了科学基础。
bZIP is a key transcription factor in the field of plant stress resistance, capable of regulating various biological processes in cell biology, including growth, development, stress response, and metabolic regulation. To investigate the function and regulatory mechanism of the HtbZIP60 gene in Houttuynia cordata thunb, total RNA was extracted from Houttuynia cordata thunbas the material, and the first strand of cDNA was synthesized. Subsequently, the HtbZIP60 gene from Houttuynia cordata thunb was successfully cloned, and relevant bioinformatics analysis was conducted. The re-sults revealed that the coding sequence of HtbZIP60 is 996 bp in length, encoding 331 amino acids, with a protein molecular weight of 37447.00 ku. It lacks a signal peptide cleavage site but contains a transmembrane structure domain, predominantly composed of α-helices. The protein is localized in the cell nucleus and possesses 30 known functional promoters. These findings provide a scientific foundation for further exploring the biological functions of HtbZIP60 in the growth and development of Houttuynia cordata thunb.
| [1] | 胡汝晓, 肖冰梅, 谭周进, 等. 鱼腥草的化学成分及其药理作用[J]. 中国药业, 2008(8): 23-25. |
| [2] | 国家中医药管理局《中华本草》编委会. 中华本草[M]. 上海: 上海科学技术出版社, 1999: 3-417. |
| [3] | 王慧玲, 崔伟, 秦鑫, 等. 鱼腥草对致热大鼠下丘脑cAMP和腹中隔区精氨酸加压素含量的影响[J]. 中国临床药理学与治疗学, 2007(1): 78-81. |
| [4] | 洪佳璇, 高雅文, 汪柏尧, 冯丙江, 莫金海, 王砚, 徐小青, 姚红伊, 赵玮, 唐法娣. 鲜鱼腥草提取物抗菌作用的实验研究[J]. 中国中医药科技, 2007(6): 418. |
| [5] | 杨慧, 李剑琦, 杨斌, 冷红文, 何士勤, 俞红, 周南进. 鱼腥草抗甲Ⅰ型流感病毒诱导细胞程序化死亡的初步研究[J]. 江西医药, 2006(12): 960-961. |
| [6] | 王金英, 丁峰, 潘介春, 张树伟, 杨亚涵, 黄幸, 范志毅, 李琳, 王颖. 植物bZIP转录因子家族的研究进展[J]. 热带农业科学, 2019, 39(6): 39-45. |
| [7] | Glover, J.M. and Harrison, S.C. (1995) Crystal Structure of the Heterodimeric bZIP Transcription Factor cFos-c-Jun Bound to DNA. Nature, 373, 257-261. https://doi.org/10.1038/373257a0 |
| [8] | 龚道勇. 慈竹bZlP和NRT基因克隆及功能初步研究[D]: [硕士学位论文]. 锦阳: 西南科技大学, 2018. |
| [9] | 刘莉. DREBs、bZIP转录因子与植物抗旱性研究进展[J]. 浙江农业科学, 2013(1): 98-102. |
| [10] | Takahashi, H., Kawakatsu, T. and Wakasa, Y. (2012) A Rice Transmembrane bZIP Transcription Factor, OsbZIP39, Regulates the Endoplasmic Reticulum Stress Response. Plant and Cell Physiology, 53, 144-153.
https://doi.org/10.1093/pcp/pcr157 |
| [11] | Yoshida, T., Fujita, Y. and Maruyama, K. (2015) Four Arabidopsis AREB/ABF Transcription Factors Function Predominantly in Gene Expression Downstream of SnRK2 Kinases in Ab-scisic Acid Signalling in Response to Osmotic Stress. Plant, Cell & Environment, 38, 35-49. https://doi.org/10.1111/pce.12351 |
| [12] | Moreno, A.A., Mukhtar, M.S., Blanco, F., et al. (2012) IRE1/bZIP60-Mediated Unfolded Protein Response Plays Distinct Roles in Plant Immunity and Abiotic Stress Respons-es. PLOS ONE, 7, e31944.
https://doi.org/10.1371/journal.pone.0031944 |
| [13] | Nagashima, Y., Iwata, Y., Ashida, M., et al. (2014) Exogenous Salicylic Acid Activates Two Signaling Arms of the Unfolded Protein Response in Arabidopsis. Plant, Cell & Environ-ment, 55, 1772-1778.
https://doi.org/10.1093/pcp/pcu108 |
| [14] | Li, Z., Tang, J., Srivastava, R., et al. (2020) The Transcription Factor bZIP60 Links the Unfolded Protein Response to the Heat Stress Response in Maize. Plant Cell, 32, 3559-3575. https://doi.org/10.1105/tpc.20.00260 |
| [15] | Kaur, N. and Kandoth, P.K. (2021) Tomato bZIP60 mRNA Undergoes Splicing in Endoplasmic Reticulum Stress and in Response to Environmental Stresses. Plant Physiology and Biochemis-try, 160, 397-403.
https://doi.org/10.1016/j.plaphy.2021.01.033 |
