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Botanical Research 2024
快速测定植物中可溶性糖含量的方法改良
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Abstract:
可溶性糖含量变化是植物响应逆境胁迫环境的重要指标之一，快速准确检测植物中可溶性糖含量的变化为植物抗逆性能研究提供了技术基础。传统分光光度法测定可溶性糖含量基于以前的实验室配置条件开展，存在测定繁琐和耗时长等问题。本研究基于目前实验室配置条件，分别从植物材料收集、破碎、测定等环节对传统测定方法进行改进，在保证了实验严谨性的前提下，有效提高了实验效率。方法验证结果显示，改进后方法的线性回归方程式为y = 1.2411x + 0.2236，线性相关系数为0.9993，检出限为7.1306 μg/mL，测定下限为28.5224 μg/mL，精密度RSD值小于2%，样品加标回收率为97%~103%之间，验证了改进后方法的可行性。方法应用结果显示，拟南芥和水稻幼苗在冷胁迫下可溶性糖含量均显著升高。改进后的分光光度法测定可溶性糖含量的方法快速准确，适合多个样品的同时测定，可作为可溶性糖含量测定的快速分析方法。
The change of soluble sugar content is one of the important indicators for evaluating plant re-sistance in respond to stress environments, and the rapid and accurate detection of soluble sugar content in plants provides a technical basis for studying plant resistance to stress. The traditional method based on the previous laboratory condition is cumbersome and time-consuming. Based on the current laboratory condition, improvements have been made from the aspects of plant material collection, crushing, and detection, which effectively improve the experimental efficiency under the premise of ensuring the rigor of the experiment. The validation results showed a linear regression equation of y = 1.2411x + 0.2236, a linear correlation coefficient of 0.9993, a detection limit of 7.1306 μg/mL, a determination limit of 28.5224 μg/mL, a precision RSD value of less than 2%, and a sample recovery rate of 97%-103%, which proved the feasibility of the improved method. The ap-plication results showed that the soluble sugar content in the seedlings of Arabidopsis and rice seedlings are both significantly increased under cold stress. The improved spectrophotometric method for determining soluble sugar content is fast and accurate, suitable for simultaneous de-termination of multiple samples, and can be used for rapid determination of soluble sugar content.
| [1] | Sami, F., Yusuf, M., Faizan, M., Faraz, A. and Hayat, S. (2016) Role of Sugars under Abiotic Stress. Plant Physiology and Biochemistry, 109. 54-61. https://doi.org/10.1016/j.plaphy.2016.09.005 |
| [2] | 刘瑞峰, 刘卫东, 舒必超, 等. 低温胁迫对假俭草SOD酶、MDA及可溶性糖含量的影响[J]. 湖南林业科技, 2012, 39(6): 8-10. |
| [3] | 梁锁兴, 孟庆仙, 石美娟, 等. 平欧榛枝条可溶性蛋白及可溶性糖含量与抗寒性关系的研究[J]. 中国农学通报, 2015, 31(13): 14-18. |
| [4] | 张立磊, 毛达, 郑树景, 等. 红叶碧桃抗寒性指标的测定分析[J]. 资源开发与市场, 2015, 31(5): 515-517. |
| [5] | 林艳, 郭伟珍, 徐振华, 等. 大叶女贞抗寒性及冬季叶片丙二醛和可溶性糖含量的变化[J]. 中国农学通报, 2012, 28(25): 68-72. |
| [6] | 田宝. 干旱胁迫对大叶黄杨保护酶及渗透物质含量的影响[J]. 山东林业科技, 2019(4): 64-67. |
| [7] | Zhao, H.Y., Sun, S.M., Zhang, L.H., et al. (2020) Carbohydrate Metabolism and Transport in Apple Roots under Nitrogen Deficiency. Plant Physiology and Biochemistry, 155, 455-463. https://doi.org/10.1016/j.plaphy.2020.07.037 |
| [8] | Maness, N. (2010) Extraction and Analysis of Soluble Carbohy-drates. In: Sunkar, R. Ed., Plant Stress Tolerance. Methods in Molecular Biology, Humana Press, 639, 341-370. https://doi.org/10.1007/978-1-60761-702-0_22 |
| [9] | Wang, H., Gong, M., Xin, H., et al. (2018) Effects of Chilling Stress on the Accumulation of Soluble Sugars and Their Key Enzymes in Jatropha curcas Seedlings. Physiology and Molecular Biology of Plants, 24, 857-865.
https://doi.org/10.1007/s12298-018-0568-6 |
| [10] | 刘松, 赵振宇, 赵尚碧, 等. UPLC-Q/OrbitrapHRMS测定大曲中5种糖和甘露糖醇[J]. 中国酿造, 2020(39): 197-200. |
| [11] | 刘兴禄, 王红平, 孙文泰, 等. 5个砧木苹果枝条的抗寒性评价[J]. 果树学报, 2021, 38(8): 1264-1274. |
| [12] | 由继红, 董春光, 史晓昆. 小麦叶片可溶性糖含量测定方法的研究[J]. 实验室科学, 2021, 24(2): 27-29. |
| [13] | Sun, C., Zhu, L., Cao, L., Qi, H., Liu, H., Zhao, F. and Han, X. (2022) PKS5 Confers Cold Tolerance by Controlling Stomatal Movement and Regulating Cold-Responsive Genes in Arabidopsis. Life, 12, Article 1633.
https://doi.org/10.3390/life12101633 |
| [14] | Wu, Z., Guo, Z., Wang, K., et al. (2023) Comparative Metabolomic Analysis Reveals the Role of OsHPL1 in the Cold-Induced Metabolic Changes in Rice. Plants, 12, Article 2032. https://doi.org/10.3390/plants12102032 |
| [15] | 邹琪. 植物生理学实验指导[M]. 北京: 中国农业出版社, 2017. |
| [16] | 邢书才, 李风华, 赵彦辉, 等. 分光光度法测定水中铝的比对分析及方法评价[J]. 应用化工, 2018, 47(1): 199-202. |
