Medicinal leeches have been utilized in therapy for
thousands of years. However, the adaptation physiology between leeches and
hosts is not fully understand. To disclose
the molecular mechanisms of adaptation between leech and host, the
body transcriptomes of hunger and fed blood-sucking Poecilobdellajavanica, Haemadipsacavatuses, and Hirudonipponia leeches were obtained by RNA sequencing, after comparison, a stratified
unigenes group was obtained, which closely correlated to body distension. In
the group, Rfamide receptor decreased significantly (P < 0.05) while
serotonin receptor increased significantly (P < 0.05). Moreover, four KEGG
(Kyoto Encyclopedia of Genes and Genomes) pathways, including cardiac
muscle contraction, complement and coagulation cascades,
renin-angiotensin system, and hypertrophic cardiomyopathy were significantly
enriched. The unigenes annotation, neuroregulators correlation analysis and
induced function of the KEGG pathways, were consistently supported the same
result as: vasoconstriction and systole reaction enhance in hunger leeches and vice
versa vasodilation and diastole increase in fed leeches, meanwhile,
Interspecific comparison and correlative analyses of physiological function
showed that the strongest reaction of induced heart failure from four KEGG
occur in strongest reaction of systole in hungry P.javanica and in strongest reaction of diastole in fed H.nipponia. Overall,
heart failure is likely a physiological function involved in
feeding behaviour.
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