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基于生物信息学分析肝硬化与miR-125a-5p和miR-125b-5p的相关研究
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Abstract:
目的：基于生物信息学分析肝硬化与miR-125a-5p和miR-125b-5p的功能富集通路以及关键基因，以期为肝硬化诊断与治疗提供新思路。方法：通过miRDB、miRWalk和TargetScan数据库获得miR-125a-5p和miR-125b-5p的靶基因，从GEO数据库获得肝硬化相关数据集GSE14323和GSE25097，筛选正常组织样本和肝硬化组织样本差异表达基因，将GSE25097和GSE14323所得差异表达基因取交集，获得肝硬化数据集的共表达基因，将miR-125a-5p和miR-125b-5p的靶基因与肝硬化数据集GSE14323和GSE25097的共表达基因取交集得到核心基因，对核心基因进行GO和KEGG富集以及构建PPI网络互作图。结果：GO富集分析中主要在糖氧代谢过程和线粒体外膜的活性中富集，KEGG富集分析中主要在脂肪酸生物合成和类固醇生物代谢等中富集，通过构建PPI网络互作图，得到miR-125a-5p和miR-125b-5p与肝硬化相关的4个关键基因NPL、H6PD、KIAA0319L和RFX5。结论：得到的相关通路以及4个关键基因NPL、H6PD、KIAA0319L和RFX5以期为肝硬化发病机制以及肝硬化发生发展过程中相关分子靶点和早期筛查的手段提供新思路，从而指导疾病诊疗计划和管理。
Objective: To analyze the functional enrichment pathway and key genes of miR-125a-5p and miR-125b-5p in cirrhotic patients based on bioinformatics, so as to provide new ideas for the diagnosis and treatment of cirrhosis. Methods: The target genes of miR-125a-5p and miR-125b-5p were obtained from miRDB, miRWalk and TargetScan databases, the differentially expressed genes were screened from normal and cirrhotic tissues, and the differentially expressed genes from GSE25097 and GSE14323 were crossed to obtain the co-expressed genes from the cirrhotic data set, the target genes of miR-125a-5p and miR-125b-5p were intersected with the co-expressed genes of liver cirrhosis data sets GSE14323 and GSE25097 to obtain core genes, which were enriched by GO and KEGG, and a PPI network interaction map was constructed. Results: GO was enriched in the processes of glucose and oxygen metabolism and the activities of Outer mitochondrial membrane. KEGG was enriched in the biosynthesis of fatty acids and steroids, four key genes of miR-125a-5p and miR-125b-5p related to liver cirrhosis, NPL, H6PD, KIAA0319L and RFX5, were obtained by constructing PPI network interaction maps. Conclusion: The related pathways and four key genes, NPL, H6PD, KIAA0319L and RFX5, are expected to provide new ideas for the pathogenesis of liver cirrhosis, molecular targets and early screening of liver cirrhosis, to guide the disease diagnosis and treatment planning and management.
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