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苗药大蝎子草标准汤剂对大鼠骨关节炎的作用及机制
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Abstract:
目的：探讨苗药大蝎子草标准汤剂对大鼠骨关节炎的作用及机制。方法：通过膝关节腔注射6%木瓜蛋白酶和0.09 mol/L L-半胱氨酸的混合溶液的方法建立大鼠骨关节炎(OA)模型。给药治疗4周，HE染色观察膝关节组织病变情况；ELISA检测滑膜组织中TNF-α、IL-1β水平；qRT-PCR检测膝关节软骨组织Sox-9、MMP13、Colx mRNA表达。结果：与模型组相比，大蝎子草标准汤剂低、中、高剂量各组大鼠体重均高于模型组，低剂量组和高剂量组大鼠体重显著高于模型组(P < 0.05)；给药治疗各组大鼠膝关节病变情况得到不同程度的改善；给药治疗各组大鼠滑膜组织中TNF-α水平(除中剂量组外)、IL-1β水平均极显著性低于模型组(P < 0.01)；给药治疗各组软骨组织中Sox-9 mRNA表达量(除低剂量组外)均显著高于模型组(P < 0.05)，MMP13、Colx mRNA表达量均极显著低于模型组(P < 0.01)。结论：苗药大蝎子草标准汤剂不同剂量组对OA大鼠均具有一定的保护作用；其作用机制可能与增高软骨组织中Sox-9 mRNA的表达量和降低MMP13、Colx mRNA的表达量有关。
Objective: To investigate the effect and mechanism of standardized soup of Hmong medicine Great Scorpion Grass on osteoarthritis in rats. Methods: A combination of 0.09 mol/L L-cysteine and 6% papain was injected into the knee joint cavity of rats to create an osteoarthritis (OA) model. The drug was administered and treated for 4 weeks, to observe the knee joint tissue lesions by HE staining; detect TNF-α, IL-1β levels in synovial tissue by ELISA and Sox-9, MMP13, and Colx mRNA expressions in knee cartilage tissues by qRT-PCR. Results: Rats in the low, medium, and high dose groups of Great Scorpion Grass Standard Soup had higher body weights than the model group, with the low and high dose groups significantly higher than the model group (P < 0.05); rats in each group shown varying degrees of improvement in knee joint injuries as compared to the model group; rat synovial tissues in all treatment groups had significantly lower levels of TNF-α and IL-1β than the model group (P < 0.01), with the exception of the medium-dose group; rats’ levels of Sox-9 mRNA expression in their cartilage tissues were significantly higher than those of the model group (P < 0.05), and their levels of MMP13 and Colx mRNA expression were significantly lower than those in the model group (P < 0.01). Conclusion: The different dose groups of the standard soup of Hmong medicine Da Scorpion Cao have certain protective effects on OA rats; its mode of action could be as-sociated with the upregulation of Sox-9 mRNA and downregulation of MMP13 and Colx mRNA in car-tilage tissues.
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