Introduction:
Collagen is the primary structural protein fibroblasts produce in the skin’s
extracellular matrix. Infiltration of neutrophils into the epidermis and dermis
by exposure to UV causes collagen damage and contributes to photoaging. Methods: To study the combined
effect of Lumenato and ceramide in preventing collagen-1 damage induced
by phagocytes, we used co-cultures of normal
human dermal fibroblasts (fibroblasts) and activated human neutrophils.
The present study aimed to determine the protective effect of the combination
of Lumenato and ceramide on fibroblast collagen-1 damage induced by
neutrophils. Results: Lumenato (in the range of 6.5-208 μg/ml) or ceramide (in
the range of 0.1-50 μM) inhibited the
production of superoxides and MPO by TNFα-stimulated
neutrophils, as well as the production of NO by LPS-stimulated macrophages in a
dose-dependent manner. The combinations of Lumenato and ceramide, in low
concentrations, caused synergistic prevention
of fibroblasts’ collagen-1 damage induced by TNFα-activated neutrophils,
detected by fluorescence immunostaining and WB analysis. MPO activity in
the supernatants of the co-cultures was also synergistically inhibited. Adding
Lumenato or ceramide singly or in combinations in these low concentrations to
the fibroblast cultures did not affect the expression of collagen-1. The combinations
of Lumenato or ceramide in these concentrations also caused a synergistic inhibition of NO production by activated macrophages. Conclusions: The results suggest that combining low concentrations of Lumenato and ceramide results in synergistic
protection against fibroblasts’ collagen-1 damage induced by
neutrophils, thus indicating their possible potential for enhanced skin health.
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