In
recent years, stem cells have been a focal point in research designed to
evaluate the efficacy of ophthalmologic therapies, specifically those for
corneal conditions. The corneal epithelium is one of the few regions of the
body that maintains itself using a residual stem cell population within the
adjacent limbus. Stem cell movement has additionally captivated the minds of
researchers due to its potential application in different body regions. The
cornea is a viable model for varying methods to track stem cell migratory
patterns, such as lineage tracing and live imaging from the limbus. These
developments have the potential to pave the way for future therapies designed
to ensure the continuous regeneration of the corneal epithelium following
injury via the limbal stem cell niche. This literature review aims to analyze
the various methods of imaging used to understand the limbal stem cell niche
and possible future directions that might be useful to consider for the better
treatment and prevention of disorders of the cornea and corneal epithelium.
References
[1]
Sridhar, M.S. (2018) Anatomy of Cornea and Ocular Surface. Indian Journal of Ophthalmology, 66, 190-194. https://doi.org/10.4103/ijo.IJO_646_17
[2]
Szliter-Berger, E.A. and Hazlett, L.D. (2010) Corneal Epithelium: Response to Infection. In: Dartt, D.A., Eds., Encyclopedia of the Eye, Elsevier, Amsterdam, 442-448. https://www.sciencedirect.com/science/article/abs/pii/B9780123742032000610?via%3Dihub
[3]
Ludwig, P.E., Lopez, M.J. and Sevensma, K.E. (2023) Anatomy, Head and Neck, Eye Cornea. StatPearls Publishing, Treasure Island. https://www.ncbi.nlm.nih.gov/books/NBK470340/
[4]
Eghrari, A.O., Riazuddin, S.A. and Gottsch, J.D. (2015) Overview of the Cornea: Structure, Function, and Development. Progress in Molecular Biology and Translational Science, 134, 7-23. https://doi.org/10.1016/bs.pmbts.2015.04.001
[5]
Chang, A.Y. and Purt, B. (2023) Biochemistry, Tear Film. StatPearls Publishing, Treasure Island. https://www.ncbi.nlm.nih.gov/books/NBK572136/
[6]
West-Mays, J.A. and Dwivedi, D.J. (2006) The Keratocyte: Corneal Stromal Cell with Variable Repair Phenotypes. The International Journal of Biochemistry & Cell Biology, 38, 1625-1631. https://doi.org/10.1016/j.biocel.2006.03.010
[7]
Sosnova-Netukova, M., Kuchynka, P. and Forrester, J.V. (2006) The Suprabasal Layer of Corneal Epithelial Cells Represents the Major Barrier Site to the Passive Movement of Small Molecules and Trafficking Leukocytes. British Journal of Ophthalmology, 91, 372-378. https://doi.org/10.1136/bjo.2006.097188
[8]
Espana, E.M. and Birk, D.E. (2020) Composition, Structure and Function of the Corneal Stroma. Experimental Eye Research, 198, Article ID: 108137. https://doi.org/10.1016/j.exer.2020.108137
[9]
Tuft, S.J. and Coster, D.J. (1990) The Corneal Endothelium. Eye, 4, 389-424. https://doi.org/10.1038/eye.1990.53
[10]
Sunderland, D.K. and Sapra, A. (2023) Physiology, Aqueous Humor Circulation. StatPearls Publishing, Treasure Island. https://www.ncbi.nlm.nih.gov/books/NBK553209/
Watt, F.M. and Driskell, R.R. (2010) The Therapeutic Potential of Stem Cells. Philosophical Transactions of the Royal Society B: Biological Sciences, 365, 155-163. https://doi.org/10.1098/rstb.2009.0149
[13]
Farrelly, O., Suzuki-Horiuchi, Y., Brewster, M., Kuri, P., Huang, S., Rice, G., Bae, H., Xu, J., Dentchev, T., Lee, V. and Rompolas, P. (2021) Two-Photon Live Imaging of Single Corneal Stem Cells Reveals Compartmentalized Organization of the Limbal Niche. Cell Stem Cell, 28, 1233-1247.e4. https://doi.org/10.1016/j.stem.2021.02.022
[14]
de Oliveira, R.C. and Wilson, S.E. (2020) Descemet’s Membrane Development, Structure, Function, and Regeneration. Experimental Eye Research, 197, Article ID: 108090. https://doi.org/10.1016/j.exer.2020.108090
[15]
Amitai-Lange, A., Altshuler, A., Bubley, J., Dbayat, N., Tiosano, B. and Shalom-Feuerstein, R. (2014) Lineage Tracing of Stem and Progenitor Cells of the Murine Corneal Epithelium. Stem Cells, 33, 230-239. https://doi.org/10.1002/stem.1840
[16]
Bunya, V.Y. and Puente, M.A. (2023) Corneal Donation. American Academy of Ophthalmology, San Francisco. https://eyewiki.aao.org/Corneal_Donation
[17]
Cotsarelis, G., Cheng, S.-Z., Dong, G., Sun, T.-T. and Lavker, R.M. (1989) Existence of Slow-Cycling Limbal Epithelial Basal Cells That Can Be Preferentially Stimulated to Proliferate: Implications on Epithelial Stem Cells. Cell, 57, 201-209. https://doi.org/10.1016/0092-8674(89)90958-6
[18]
Eberwein, P. and Reinhard, T. (2015) Concise Reviews: The Role of Biomechanics in the Limbal Stem Cell Niche: New Insights for Our Understanding of This Structure. Stem Cells, 33, 916-924. https://doi.org/10.1002/stem.1886
[19]
Di Girolamo, N., Bobba, S., Raviraj, V., Delic, N.C., Slapetova, I., Nicovich, P.R., Halliday, G.M., Wakefield, D., Whan, R. and Lyons, J.G. (2014) Tracing the Fate of Limbal Epithelial Progenitor Cells in the Murine Cornea. Stem Cells, 33, 157-169. https://doi.org/10.1002/stem.1769
[20]
West, J.D. (2015) Evaluating Alternative Stem Cell Hypotheses for Adult Corneal Epithelial Maintenance. World Journal of Stem Cells, 7, 281-299. https://doi.org/10.4252/wjsc.v7.i2.281
[21]
Foudi, A., Hochedlinger, K., Van Buren, D., Schindler, J.W., Jaenisch, R., Carey, V. and Hock, H. (2008) Analysis of Histone 2B-GFP Retention Reveals Slowly Cycling Hematopoietic Stem Cells. Nature Biotechnology, 27, 84-90. https://doi.org/10.1038/nbt.1517
[22]
Amin, S., Jalilian, E., Katz, E., Frank, C., Yazdanpanah, G., Guaiquil, V.H., Rosenblatt, M.I. and Djalilian, A.R. (2021) The Limbal Niche and Regenerative Strategies. Vision, 5, Article No. 43. https://doi.org/10.3390/vision5040043
[23]
Yoon, J.J. (2014) Limbal Stem Cells: Central Concepts of Corneal Epithelial Homeostasis. World Journal of Stem Cells, 6, 391-403. https://doi.org/10.4252/wjsc.v6.i4.391
[24]
Zhao, X., Das, A.V., Thoreson, W.B., James, J., Wattnem, T.E., Rodriguez-Sierra, J. and Ahmad, I. (2002) Adult Corneal Limbal Epithelium: A Model for Studying Neural Potential of Non-Neural Stem Cells/Progenitors. Developmental Biology, 250, 317-331
[25]
Yazdanpanah, G., Haq, Z., Kang, K., Jabbehdari, S., Rosenblatt, M.L. and Djalilian, A.R. (2019) Strategies for Reconstructing the Limbal Stem Cell Niche. The Ocular Surface, 17, 230-240. https://doi.org/10.1016/j.jtos.2019.01.002
Higa, K., Higuchi, J., Kimoto, R., Miyashita, H., Shimazaki, J., Tsubota, K. and Shimmura, S. (2020) Human Corneal Limbal Organoids Maintaining Limbal Stem Cell Niche Function. Stem Cell Research, 49, Article ID: 102012. https://doi.org/10.1016/j.scr.2020.102012
[28]
Foster, J.W., Wahlin, K., Adams, S.M., Birk, D.E., Zack, D.J. and Chakravarti, S. (2017) Cornea Organoids from Human Induced Pluripotent Stem Cells. Scientific Reports, 7, Article No. 41286. https://doi.org/10.1038/srep41286
[29]
Jiang, L.-L. and Liu, L. (2020) Effect of Metformin on Stem Cells: Molecular Mechanism and Clinical Prospect. World Journal of Stem Cells, 12, 1455-1473. https://doi.org/10.4252/wjsc.v12.i12.1455
[30]
HerminaStrungaru, M., Ali, A., Rootman, D. and Mireskandari, K. (2017) Endothelial Keratoplasty for Posterior Polymorphous Corneal Dystrophy in a 4-Month-Old Infant. American Journal of Ophthalmology Case Reports, 7, 23-26. https://doi.org/10.1016/j.ajoc.2017.05.001
[31]
Choi, S.O., Jeon, H.S., Hyon, J.Y., et al. (2015) Recovery of Corneal Endothelial Cells from Periphery after Injury. PLOS ONE, 10, e0138076. https://doi.org/10.1371/journal.pone.0138076