The preservation of anatomical pieces in Veterinary Anatomy is essential since it is not possible to dissect all domestic species. Most techniques use reagents with high levels of toxicity such as formaldehyde. The objective of this work was to develop a new preservation technique that uses reagents with zero toxicity and that allows obtaining preserved pieces suitable for anatomical studies. The alcohol propylene glycol technique was developed, the method of which uses a fixation step with alcohol, sodium chloride, commercial vinegar and subsequently the impregnation of the preservation solution made from propylene glycol and commercial vinegar, which are non-toxic. As a result of this work, adequately preserved sheep hearts were obtained that preserved their morphology with slight changes in size and weight, which did not affect their external and internal anatomical structure. Its coloration was not substantially affected, remaining a little lighter. The pieces obtained showed flexibility which allowed dissections to be carried out. The time to develop the technique was 20 days. A comparative study was carried out with the phenolated glycerin technique that uses toxic reagents (formaldehyde and phenol) and the pieces obtained with the alcohol propylene glycol technique were of better quality, observing that the pieces with phenolated glycerin tend to darken and are more rigid. And the time to develop the technique is 24 days. In conclusion, a preservation technique for anatomical pieces was developed that allowed the preservation of the organs under study, which allow their use for anatomical studies, and which have been preserved without changes until the time of this publication (8 months) and there are pieces preserved with this technique for 2 years.
References
[1]
García, T.C.G., Soto, Z.C.I., Oliver, G.M.R., Garrido, F.G.I. and Rodríguez, S.L.M. (2020) Piezas anatómicas preservadas como instrumento de enseñanza de la anatomía veterinaria. Latin American Journal of Science Education, 7, Article No. 12004. https://www.lajse.org/may20.html
[2]
Nieto, B.J.L., García, T.C.G., Pichardo, M.M.R., Reyes, S.A.L. and Soto, Z.C.I. (2014) Técnicas para preservar piezas anatómicas. FES-Cuautitlán UNAM, México.
[3]
Silva, R.M., Matera, J.M. and Ribeiro, A.A. (2007) New Alternative Methods to Teach Surgical Techniques for Veterinary Medicine Students despite the Absence of Living Animals. Is That an Academic Paradox? Anatomia Histolologia Embryologia, 36, 220-224. https://doi.org/10.1111/j.1439-0264.2007.00759.x
[4]
Ramírez, G.A.A.A. (2019) Redescubriendo metodologías para la recuperación y conservación de material anatomopatológico en los laboratorios de la Universidad Nacional. Morfolia, 11, 51-70. https://revistas.unal.edu.co/index.php/morfolia/article/view/84070/73203
[5]
Denis-Rodríguez, E. and Aguirre-Gutiérrez, A.A. (2017) Método Thiel Soft-Fix para la preservación de cadáveres a largo plazo. Revista Mexicana de Medicina Forense, 3, 91-98.
[6]
Pereyra, C.F. (2019) Estudio de una técnica de conservación de cadáveres sin el uso de formaldehido: Evaluación en el miembro pelviano del conejo (Oryctolagus cuniculus). Doctoral Thesis, Facultad de Ciencias Veterinarias, Universidad Nacional de Rosario, Rosario. https://rephip.unr.edu.ar/items/86f922d1-bf96-4380-90bb-4a7ce74c97cb
[7]
Wolff, D., Villa, P., Neirreitter, A., Ruibal, C., Ugon, G.A., Salgado, G. and Cantín, M. (2012) Estudio comparativo entre soluciones conservadoras con y sin formol en placenta humana. International Journal of Morphology, 30, 432-438. https://doi.org/10.4067/S0717-95022012000200013
[8]
Muñetón, G.C.A. and Ortiz, J.A. (2011) Conservación y elaboración de piezas anatómicas con sustancias diferentes al formol en la Facultad de Ciencias Agropecuarias de la Universidad de La Salle. Revista de Medicina Veterinaria, 22, 51-55. https://doi.org/10.19052/mv.558
[9]
García, C.R.X., Rodríguez, C.E.M., Barajas, M.A., Ortiz, O.Y. and Dávila, L.R. (2022) Técnicas para la preparación y preservación de piezas anatómicas y cadáveres completos. Universidad de Guadalajara. Ed. Centro Universitario del Sur. http://www.cusur.udg.mx/es/sites/default/files/adjuntos/Tecnicasparalapreparacionypreservaciondepiezasanatomicasycadaverescompletos-Ebook.pdf
[10]
Von Haegens, G. (1979) Impregnation of Soft Biological Specimens Whit Thermosetting Resins and Elastomers. Anatomical Record, 194, 247-255. https://doi.org/10.1002/ar.1091940206
[11]
Sánchez, C.C., Andromaco, M., Páez, R., Barello, M.R. and Pedernera, G. (2012) Estudio de nuevas técnicas para conservación de piezas anatómicas. Plastinación. Revista de Salud Pública, 3, 27-32.
[12]
Riederer, B.M. (2014) Plastination and Its Importance in Teaching Anatomy. Critical Points for Long-Term Preservation of Human Tissue. Journal of Anatomy, 224, 309-315. https://doi.org/10.1111/joa.12056
[13]
Peralta, P.E., Beltrán, G.J.A., Luque, B.R.M. and Quijano, B.Y. (2017) La plastinación como técnica de preservación de material biológico para docencia e investigación en anatomía. Morfolia, 9, 55-62. https://repository.urosario.edu.co/handle/10336/28386
[14]
Pedraza, M., Ochoa, A., Vargas, V., Aldana, D., Pedraza, M., Castaño, M.J., Ruiz, M. and Moreno, M. (2020) Conservación de órganos con el uso de poliuretano: Prueba de laboratorio en corazón de cerdos. Morfolia, 12, 31-55. https://revistas.unal.edu.co/index.php/morfolia/article/view/88607
[15]
Gasse, H. (2017) Nomina Anatómica Veterinaria. International Committee on Veterinary Gross Anatomic Nomenclature. 6th Edition, Chairman Committee, Hannover. https://www.wava-amav.org/wava-documents.html
[16]
Cutipa, D.C., Condemayta, Z.C., Butrón, O.O. and Rojas, M.M. (2014) Estudio comparativo de técnicas de conservación anatómica de especimenes de cadaveres ovinos en altura, utilizando soluciones de formol y prives. Revista Investigaciones Altoandinas, 16, 33-38. http://www.unap.edu.pe/oui/ria/ https://doi.org/10.18271/ria.2014.32
[17]
Boonstra, H., Oosterhuis, J.W., Oosterhuis, A.M. and Fleuren, G.J. (1983) Cervical Tissue Shrinkage by Formaldehyde Fixation, Paraffin Wax Embedding, Section Cutting and Mounting. Virchows Archiv (Pathol Anat), 402, 195-201. https://doi.org/10.1007/BF00695061
[18]
Gretser, S., Weber, K.J., Braun, Y., Harter, P.N., Rolle, U., McNally, J. and Gradhand, E. (2023) Tissue Shrinkage of Resected Specimens in Hirschsprung’s Disease: Why Pediatric Surgeons Think the Bowel Specimen was Longer than Indicated in the Pathology Report. Pediatric and Developmental Pathology, 26, 287-291. https://doi.org/10.1177/10935266231162684
[19]
Akgul, M., Arslan, A.I., Yazici, C., Altin, E. and Oznur, M. (2022) The Shrinkage Effect of Formalin on Renal Cell Carcinoma: Does It Change the Stages. Journal of the Pakistan Medical Association, 72, 2175-2179. https://doi.org/10.47391/JPMA.3107
[20]
de Waal, J. (2021) Skin Tumour Specimen Shrinkage with Excision and Formalin Fixation—How Much and Why: A Prospective Study and Discussion of the Literature. ANZ Journal of Surgery, 91, 2744-2749. https://doi.org/10.1111/ans.17109
[21]
Kansu, L., Aydın, E., Akkaya, H., Avcı, S. and Akalın, N. (2017) Shrinkage of Nasal Mucosa and Cartilage during Formalin Fixation. Balkan Medical Journal, 34, 458-463. https://doi.org/10.4274/balkanmedj.2015.1470
[22]
Holda, M.K., Klimek-Piotrowska, W., Kozjej, M., Piatek, K. and Holda, J. (2016) Influence of Different Fixation Protocols on the Preservation and Dimensions of Cardiac Tissue. Journal of Anatomy, 229, 334-340. https://doi.org/10.1111/joa.12469
[23]
Cabrera, N.C., Espinoza, J.R., Vargas-Jentzsch, P., Sandoval, P., Ramos, L.A. and Aponte, P.M. (2017) Alcohol-Based Solutions for Bovine Testicular Tissue Fixation. Journal of Veterinary Diagnostic Investigation, 29, 91-99. https://doi.org/10.1177/1040638716672252
