An in vitro experiment was conducted to evaluate the effects of grain type on in vitro dry matter disappearance (IVDMD) and pH using ruminal fluid and a buffer reagent. Five beef cows were used for ruminal fluid collection and ruminal fluid was pooled prior to use. The cows used for ruminal fluid collection were maintained on a forage-based diet (60:40 forage to concentrate) for 28 d prior to ruminal fluid collection. Three grain types were evaluated: 1) dry corn (89% dry matter; DRC), 2) high-moisture corn (72% dry matter; HMC) and 3) rye (89% dry matter; RYE). Data were analyzed as a completely randomized design. A total of twenty-one replications of each grain type were used for statistical analysis (n = 63 tubes total). IVDMD was greater (P ≤ 0.05) by 97.1% and 46.4% for RYE compared to DRC and HMC, respectively. Additionally, IVDMD was greater (P = 0.05) by 34.6% for HMC compared to DRC. Final pH was decreased (P ≤ 0.05) for RYE by 12.3% and 2.8% for RYE compared to DRC and HMC, respectively. Also, final pH was decreased (P = 0.05) by 9.8% for HMC compared to DRC. These data indicate that corn harvest method (dry vs. high-moisture) influences IVDMD and final pH and that rye has a greater disappearance of dry matter and lower final pH than corn under in vitro conditions.
References
[1]
Lemaire, G., Franzluebbers, A., Carvalho, P.C.D.F. and Dedieu, B. (2014) Integrated Crop-Livestock Systems: Strategies to Achieve Synergy between Agricultural Production and Environmental Quality. Agriculture, Ecosystems & Environment, 190, 4-8. https://doi.org/10.1016/j.agee.2013.08.009
[2]
Bowles, T.M., Mooshammer, M., Socolar, Y., Calderón, F., Cavigelli, M.A., Culman, S.W., Deen, W., Drury, C.F., Garcia Y Garcia, A., Gaudin, A.C.M., Harkcom, W.S., Lehman, R.M., Osborne, S.L., Robertson, G.P., Salerno, J., Schmer, M.R., Strock, J. and Grandy, A.S. (2020) Long-Term Evidence Shows That Crop-Rotation Diversification Increases Agricultural Resilience to Adverse Growing Conditions in North America. One Earth, 2, 284-293. https://doi.org/10.1016/j.oneear.2020.02.007
[3]
Mould, F.L. (2003) Predicting Feed Quality—Chemical Analysis and in Vitro Evaluation. Field Crops Research, 84, 31-44. https://doi.org/10.1016/S0378-4290(03)00139-4
[4]
Tilley, J.M.A. and Terry, R.A. (1963) A Two-Stage Technique for the in Vitro Digestion of Forage Crops. Grass and Forage Science, 18, 104-111. https://doi.org/10.1111/j.1365-2494.1963.tb00335.x
[5]
McDougall, E.I. (1948) Studies on Ruminant Saliva. 1. The Composition and Output of Sheep’s Saliva. Biochemical Journal, 43, 99-109. https://doi.org/10.1042/bj0430099
[6]
Krieg, J., Seifried, N., Steingass, H. and Rodehutscord, M. (2017) In Situ and in Vitro Ruminal Starch Degradation of Grains from Different Rye, Triticale and Barley Genotypes. Animal, 11, 1745-1753. https://doi.org/10.1017/S1751731117000337
[7]
Rajtar, P., Górka, P., Schwarz, T. and Micek, P. (2020) Effect of Hybrid Rye and Maize Grain Processing on Ruminal and Postruminal Digestibility Parameters. Annals of Animal Science, 20, 1065-1083. https://doi.org/10.2478/aoas-2020-0025
[8]
Rajtar, P., Górka, P., Śliwiński, B., Wieczorek, J., Boros, D. and Micek, P. (2020) Effect of Rye Grain Derived from Different Cultivars or Maize Grain Use in the Diet on Ruminal Fermentation Parameters and Nutrient Digestibility in Sheep. Annals of Animal Science, 21, 959-976. https://doi.org/10.2478/aoas-2020-0118
[9]
Kozubek, A. and Tyman, J.H. (1999) Resorcinolic Lipids, the Natural Non-Isoprenoid Phenolic Amphiphiles and Their Biological Activity. Chemical Reviews, 99, 1-26. https://doi.org/10.1021/cr970464o
[10]
Cooper, R.J., Milton, C.T., Klopfenstein, T.J., Scott, T.L., Wilson, C.B. and Mass, R.A. (2002) Effect of Corn Processing on Starch Digestion and Bacterial Crude Protein Flow in Finishing Cattle. Journal of Animal Science, 80, 797-804. https://doi.org/10.2527/2002.803797x
[11]
Owens, F.N. and Soderlund, S. (2006) Ruminal and Postruminal Starch Digestion By Cattle. In: Cattle Grain Processing Symposium Tulsa, Oklahoma State University, OK, 15-17.
[12]
Aschenbach, J.R., Penner, G.B., Stumpff, F. and Gäbel, G. (2011) Ruminant Nutrition Symposium: Role of Fermentation Acid Absorption in the Regulation of Ruminal pH. Journal of Animal Science, 89, 1092-1107. https://doi.org/10.2527/jas.2010-3301
[13]
Schwaiger, T., Beauchemin, K.A. and Penner, G.B. (2013) The Duration of Time That Beef Cattle Are Fed a High-Grain Diet Affects the Recovery from a Bout of Ruminal Acidosis: Dry Matter Intake and Ruminal Fermentation 1. Journal of Animal Science, 91, 5729-5742. https://doi.org/10.2527/jas.2013-6471
[14]
Rusche, W.C., Walker, J.A., Sexton, P., Brattain, R.S. and Smith, Z.K. (2020) Evaluation of Hybrid Rye on Growth Performance, Carcass Traits, and Efficiency of Net Energy Utilization in Finishing Steers. Translational Animal Science, 4, Txaa173. https://doi.org/10.1093/tas/txaa173
[15]
Buckhaus, E.M., Rusche, W.C. and Smith, Z.K. (2021) Effect of Complete Replacement of Dry-Rolled Corn with Unprocessed Rye on Growth Performance, Efficiency of Dietary Net Energy Use, and Carcass Traits of Finishing Heifers. Animals, 11, 99. https://doi.org/10.3390/ani11010099
[16]
Owens, F.N., Secrist, D.S., Hill, W.J. and Gill, D.R. (1998) Acidosis in Cattle: A Review. Journal of Animal Science, 76, 275-286. https://doi.org/10.2527/1998.761275x
