|
|
Botanical Research 2024
秋葵种子游离酚提取工艺优化及抗氧化活性研究
|
Abstract:
本研究以新鲜的秋葵种子为原料，基于料液比、乙醇浓度、时间和温度这四个单因素，采用正交优化实验设计的方法，确定了超声辅助醇提法提取秋葵种子游离酚的最佳提取条件。测定了秋葵种子游离酚对DPPH自由基和ABTS自由基的清除率，以及对亚铁离子的还原能力。结果表明：最优提取条件为液料比1:70、乙醇浓度70%、温度为40℃、时间为40 min时，获得秋葵种子游离酚的提取量为36.57 mg GAE/g dw。此外，秋葵种子游离酚表现出了较好的DPPH清除率和亚铁离子还原能力，同时其ABTS清除率与Vc相当。实验结果表明，秋葵种子游离酚具备较好的抗氧化活性，为进一步秋葵研究和工业化生产提供了理论支持和参考。
This study utilized fresh okra seeds as the raw material and employed an orthogonal optimization experimental design based on four single factors: solid-liquid ratio, ethanol concentration, time, and temperature, to determine the optimal extraction conditions for free phenolics from okra seeds using ultrasound-assisted alcohol extraction. The scavenging rates of the free phenolics from okra seeds against DPPH and ABTS radicals, as well as their ferric reducing antioxidant power, were measured. The results showed that the optimal extraction conditions were a solid-liquid ratio of 1:70, ethanol concentration of 70%, temperature of 40°C, and time of 40 minutes, under which the extraction yield of okra seeds free phenolics (OSFP) was 36.57 mg GAE/g dw. Additionally, the OSFP exhibited great DPPH scavenging activity and ferric ion reducing ability, while their ABTS scavenging rate was comparable to that of VC. The experimental results indicate that the OSFP possess good antioxidant activity, providing theoretical support and reference for further research and industrial production of okra.
| [1] | Elkhalifa, A.E.O., Alshammari, E., Adnan, M., et al. (2021) Okra (Abelmoschus esculentus) as a Potential Dietary Medicine with Nutraceutical Importance for Sustainable Health Applications. Molecules, 26, Article 696. https://doi.org/10.3390/molecules26030696 |
| [2] | Islam, M.T. (2019) Phytochemical Information and Pharmacological Activities of Okra (Abelmoschus esculentus): A Literature-Based Review. Phytother Phytotherapy Research, 33, 72-80. https://doi.org/10.1002/ptr.6212 |
| [3] | Wang, H., Zhao, Q.-S., Wang, X.-D., et al. (2019) Pretreatment of Ultrasound Combined Vacuum Enhances the Convective Drying Efficiency and Physicochemical Properties of Okra (Abelmoschus esculentus). LWT, 112, Article 108201. https://doi.org/10.1016/j.lwt.2019.05.099 |
| [4] | 刘连军. 黄秋葵的应用价值及栽培技术[J]. 现代农业科技, 2015(19): 101-102. |
| [5] | 单承莺, 马世宏, 张卫明. 保健蔬菜黄秋葵的应用价值与前景[J]. 2012, 31(2): 68-71. |
| [6] | Ijarotimi, O.S., Akinola-Ige, A.O. and Oluwajuyitan, T.D. (2023) Okra Seeds Proteins: Amino Acid Profile, Free Radical Scavenging Activities and Inhibition of Diabetes and Hypertensive Converting Enzymes Indices. Measurement: Food, 11, Article 100101. https://doi.org/10.1016/j.meafoo.2023.100101 |
| [7] | Wu, S., Shen, D., Wang, R., et al. (2021) Phenolic Profiles and Antioxidant Activities of Free, Esterified and Bound Phenolic Compounds in Walnut Kernel. Food Chemistry, 350, Article 129217. https://doi.org/10.1016/j.foodchem.2021.129217 |
| [8] | Xia, F., Zhong, Y., Li, M., et al. (2015) Antioxidant and Anti-Fatigue Constituents of Okra. Nutrients, 7, 8846-8858. https://doi.org/10.3390/nu7105435 |
| [9] | Wang, X., Liu, X., Shi, N., et al. (2023) Response Surface Methodology Optimization and HPLC-ESI-QTOF-MS/MS Analysis on Ultrasonic-Assisted Extraction of Phenolic Compounds from Okra (Abelmoschus esculentus) and Their Antioxidant Activity. Food Chemistry, 405 Part B, Article 134966. https://doi.org/10.1016/j.foodchem.2022.134966 |
| [10] | Peanparkdee, M., Patrawart, J. and Iwamoto, S. (2020) Physicochemical Stability and in vitro Bioaccessibility of Phenolic Compounds and Anthocyanins from Thai Rice Bran Extracts. Food Chemistry, 329, Article 127157. https://doi.org/10.1016/j.foodchem.2020.127157 |
| [11] | Zhang, M., Ma, X., Xiao, Z., et al. (2023) Polyphenols in Twenty Cultivars of Blue Honeysuckle (Lonicera caerulea L.): Profiling, Antioxidant Capacity, and Alpha-Amylase Inhibitory Activity. Food Chemistry, 421, Article 136148. https://doi.org/10.1016/j.foodchem.2023.136148 |
| [12] | Vinci, G., D’Ascenzo, F., Maddaloni, L., et al. (2022) The Influence of Green and Black Tea Infusion Parameters on Total Polyphenol Content and Antioxidant Activity by ABTS and DPPH Assays. Beverages, 8, Article 18. https://doi.org/10.3390/beverages8020018 |
| [13] | 王纪辉, 何佳丽, 耿阳阳, 等. 核桃叶多酚提取及其组成和抗氧化能力探究[J]. 中国粮油学报, 2023, 38(4): 104-112. |
| [14] | 游新勇, 崔利华, 周亚丽, 等. 响应面法优化藜麦种子中多酚提取工艺[J]. 食品工业, 2020, 41(10): 118-122. |
| [15] | 赵强, 刘乐, 杨洁, 等. 响应面法优化藜麦糠中多酚超声提取工艺及其抗氧化活性[J]. 中国粮油学报, 2020, 35(7): 143-149. |
| [16] | Shojaee-Aliabadi, S., Hosseini, S.M., Tiwari, B., et al. (2013) Polyphenols Content and Antioxidant Activity of Ghure (Unripe Grape) Marc Extract: Influence of Extraction Time, Temperature and Solvent Type. International Journal of Food Science & Technology, 48, 412-418. https://doi.org/10.1111/j.1365-2621.2012.03203.x |
| [17] | 周昂摺, 周巧婷, 周恩牧, 等. 超声波辅助萃取波罗蜜种子多酚工艺研究[J]. 浙江林业科技, 2020, 40(4): 49-53. |
| [18] | Davidov-Pardo, G., Arozarena, I. and Marín-Arroyo, M.R. (2010) Stability of Polyphenolic Extracts from Grape Seeds after Thermal Treatments. European Food Research and Technology, 232, 211-220. https://doi.org/10.1007/s00217-010-1377-5 |
| [19] | Ong, E.S., Oh, C.L.Y., Tan, J.C.W., et al. (2021) Pressurized Hot Water Extraction of Okra Seeds Reveals Antioxidant, Antidiabetic and Vasoprotective Activities. Plants, 10, Article 1645. https://doi.org/10.3390/plants10081645 |
| [20] | Rusmana, D., Wahyudianingsih, R., Elisabeth, M., et al. (2017) Antioxidant Activity of Phyllanthus Niruri Extract, Rutin and Quercetin. The Indonesian Biomedical Journal, 9, 84-90. https://doi.org/10.18585/inabj.v9i2.281 |
| [21] | Tian, C., Liu, X., Chang, Y., et al. (2021) Investigation of the Anti-Inflammatory and Antioxidant Activities of Luteolin, Kaempferol, Apigenin and Quercetin. South African Journal of Botany, 137, 257-264. https://doi.org/10.1016/j.sajb.2020.10.022 |
