The work investigated the secondary metabolites present in different parts ofthePandiaka heudelotii plant harvested from Ezira, Orumba south local government area of Anambra state, southeastern Nigeria as well as their antimicrobial activity. The objectives were to extract, determine the phytochemicals present, evaluate the antimicrobial potential and determine the zones of inhibition of the root, stem and leaf extracts of the plant. The emergence of antibiotics has decreased the spread and severity of a wide range of diseases. Plant extracts were tested for antibacterial and antifungal activities against Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Streptococcus pyogenes, Bacillus subtelis, Pseudomonas aerogenes, Salmonella typhi, Aspergillus flavus, Aspergillus niger and Candida albicans. The zone of inhibition of extracts was compared with that of standard drugs like Erythromycin, Ciprofloxacin, Fulcin and Fluconazole. The result revealed the inhibition of bacterial and fungal growth with some test organisms. The microbial activity of the plant parts may be due to the presence of various secondary metabolites. The ethyl acetate extracts of Pandiaka heudelotii stood out with a minimum inhibitory concentration (MIC) range of 5 - 10 mg/mL and a minimum bactericidal concentration (MBC)/minimum fungicidal concentration (MFC) of 10 - 20 mg/ml against some test organisms. The plant can thus be worked upon to discover biologically active natural products that may serve as a prelude to the development of new pharmaceutical research undertakings.
References
[1]
Peterson, E. and Kaur, P. (2018) Antibiotic Resistance Mechanisms in Bacteria: Relationships between Resistance Determinants of Antibiotic Producers, Environmental Bacteria, and Clinical Pathogens. Frontiers in Microbiology, 9, Article 2928 https://doi.org/10.3389/fmicb.2018.02928
[2]
Davies, J. and Davies, D. (2010) Origins and Evolution of Antibiotic Resistance. Microbiology and Molecular Biology Reviews, 74, 417-433. https://doi.org/10.1128/MMBR.00016-10
[3]
Kebede, T., Gadisa, E. and Tufa, A. (2021) Antimicrobial Activities Evaluation and Phytochemical Screening of Some Selected Medicinal Plants: A Possible Alternative in the Treatment of Multidrug-Resistant Microbes. PLOS ONE, 16, 1-16. https://doi.org/10.1371/journal.pone.0249253
[4]
Pandey, S. (2015) Preliminary Phytochemical Screening and in Vitro Antibacterial Activity of Bauhinia variegata Linn. against Human Pathogens. Asian Pacific Journal of Tropical Disease, 5, 123-129. https://doi.org/10.1016/S2222-1808(14)60639-3
[5]
Mishra, P., Sohrab, S. and Mishra, S. K. (2021) A Review on the Phytochemical and Pharmacological Properties of Hyptis suaveolens (L.) Poit. Future Journal of PharmaceuticalSciences, 7, 1-11. https://doi.org/10.1186/s43094-021-00219-1
[6]
Demain, A.L. and Fang, A. (2000) The Natural Functions of Secondary Metabolites. Advances in Biochemical Engineering/Biotechnology, 69, 1-39. https://doi.org/10.1007/3-540-44964-7_1
[7]
Ifeanacho, M.O., Ikewuchi, C.C. and Ikewuchi, J.C. (2017) Investigation of the Profile of Phenolic Compounds in the Leaves and Stems of Pandiaka heudelotii Using Gas Chromatography Coupled with Flame Ionization Detector. Food Science & Nutrition, 5, 646-652. https://doi.org/10.1002/fsn3.443
[8]
Abubakar, S., Akanbi, B., Etim, V., Segun, O. and Ogbu, J. (2016) Comparative Study of Phytochemical and Synergistic Antibacterial Activity of Tribulus terrestris (L.) and Pandiakaheudelotii (Moq.) Hien on Some Clinical Bacterial Isolates. Pharmaceutical and Biological Evaluations, 3, 83-91.
[9]
Trease, G.E. and Evans, W.T. (2002) Textbook of Pharmacology. WB Saunders Company Limited, London.
[10]
Nigussie, D., Davey, G., Legesse, B.A., Fekadu, A. and Makonnen, E. (2021) Antibacterial Activity of Methanol Extracts of the Leaves of Three Medicinal Plants against Selected Bacteria Isolated from Wounds of Lymphoedema Patients. BMC Complementary Medicine and Therapies, 21, 1-10. https://doi.org/10.1186/s12906-020-03183-0
[11]
Al-Wajih, A.M., El-Shaibany, A.M., Raweh, S.M. and El–Aasser, M.M. (2022) Preliminary Phytochemical Screening for Various Secondary Metabolites, Quantitative and Qualitative Analysis of Yemeni Aloe vera and Aloe vacillans Flower Extracts. GSC Biological and Pharmaceutical Sciences, 21, 202-210. https://doi.org/10.30574/gscbps.2022.21.2.0437
[12]
Pratama, R. and Andriani, Y. (2023) Phytochemicals Analysis of Fresh and Boiled Golden Applesnail (Pomacea canaliculata). GPH-International Journal of Agriculture and Research, 6, 1-6.
Ilondu, E.M. and Enwa, F.O. (2013) Phytochemical Screening for Compounds in Conarium schwefurthii (atile) Leaves from Jos North, Plateau State. International Journal of Pharmaceutical and Chemical Sciences, 2, 14-19.