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铜绿假单胞菌肺部感染动物模型
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Abstract:
铜绿假单胞菌是一种具有很强抗生素耐药性的条件致病菌，能够引起广泛的危及生命的急性和慢性感染。它是细菌性肺部感染的主要病原菌之一，具有很高的发病率和死亡率。建立有效、合适的动物模型能够极大地帮助我们了解疾病的发病机制以及测试和开发新的治疗策略。本文旨在通过对以铜绿假单胞菌为病原菌建立的动物肺炎模型方法的概述及评价，为基础与临床研究应用提供理论基础。
Pseudomonas aeruginosa is a highly antibiotic-resistant conditionally pathogenic bacterium capable of causing a wide range of life-threatening acute and chronic infections. It is one of the major pathogens of bacterial lung infections with high morbidity and mortality. The establishment of effective and appropriate animal models can greatly assist our understanding of disease pathogenesis as well as testing and developing new therapeutic strategies. The aim of this paper is to provide a theoretical basis for basic and clinical research applications by detailing and evaluating the methodology of animal pneumonia models established using Pseudomonas aeruginosa as the causative agent.
| [1] | Wisinger, D. (1993) Bacterial Pneumonia. S. pneumoniae and H. influenzae Are the Villains. Postgraduate Medicine, 93, 43-52. https://doi.org/10.1080/00325481.1993.11701700 |
| [2] | Kuek, L.E. and Lee, R.J. (2020) First Contact: The Role of Respiratory Cilia in Host-Pathogen Interactions in the Airways. American Journal of Physiology-Lung Cellular and Molecular Physiology, 319, L603-L619. https://doi.org/10.1152/ajplung.00283.2020 |
| [3] | Gonschior, H., Schmied, C., Van der Veen, R.E., et al. (2022) Nanoscale Segregation of Channel and Barrier Claudins Enables Paracellular Ion Flux. Nature Communications, 13, Article No. 4985. https://doi.org/10.1038/s41467-022-32533-4 |
| [4] | Ma, J., Rubin, B.K. and Voynow, J.A. (2018) Mucins, Mucus, and Goblet Cells. Chest, 154, 169-176. https://doi.org/10.1016/j.chest.2017.11.008 |
| [5] | Rokicki, W., Rokicki, M., Wojtacha, J., et al. (2016) The Role and Importance of Club Cells (Clara Cells) in the Pathogenesis of Some Respiratory Diseases. Kardiochirurgia i Torakochirurgia Polska, 13, 26-30. https://doi.org/10.5114/kitp.2016.58961 |
| [6] | Vielle, N.J., García-Nicolás, O., Oliveira Esteves, B.I., et al. (2019) The Human Upper Respiratory Tract Epithelium Is Susceptible to Flaviviruses. Frontiers in Microbiology, 10, Article 811. https://doi.org/10.3389/fmicb.2019.00811 |
| [7] | Hewitt, R.J. and Lloyd, C.M. (2021) Regulation of Immune Responses by the Airway Epithelial Cell Landscape. Nature Reviews Immunology, 21, 347-362. https://doi.org/10.1038/s41577-020-00477-9 |
| [8] | Wanner, A., Salathé, M. and O’Riordan, T.G. (1996) Mucociliary Clearance in the Airways. American Journal of Respiratory and Critical Care Medicine, 154, 1868-1902. https://doi.org/10.1164/ajrccm.154.6.8970383 |
| [9] | Button, B., Cai, L.-H., Ehre, C., et al. (2012) A Periciliary Brush Promotes the Lung Health by Separating the Mucus Layer from Airway Epithelia. Science, 337, 937-941. https://doi.org/10.1126/science.1223012 |
| [10] | Bustamante-Marin, X.M. and Ostrowski, L.E. (2017) Cilia and Mucociliary Clearance. Cold Spring Harbor Perspectives in Biology, 9, a028241. https://doi.org/10.1101/cshperspect.a028241 |
| [11] | Ganz, T. (2002) Antimicrobial Polypeptides in Host Defense of the Respiratory Tract. Journal of Clinical Investigation, 109, 693-697. https://doi.org/10.1172/JCI0215218 |
| [12] | Fahy, J.V. and Dickey, B.F. (2010) Airway Mucus Function and Dysfunction. New England Journal of Medicine, 363, 2233-2247. https://doi.org/10.1056/NEJMra0910061 |
| [13] | Bardoel, B.W. and Strijp, J.A. (2011) Molecular Battle between Host and Bacterium: Recognition in Innate Immunity. Journal of Molecular Recognition, 24, 1077-1086. https://doi.org/10.1002/jmr.1156 |
| [14] | Semeraro, E.F., Marx, L., Mandl, J., et al. (2022) Lactoferricins Impair the Cytosolic Membrane of Escherichia coli within a Few Seconds and Accumulate Inside the Cell. eLife, 11, e72850. https://doi.org/10.7554/eLife.72850 |
| [15] | Wang, J., Xu, H., Wang, D., et al. (2017) Comparison of Pathogen Eradication Rate and Safety of Anti-Bacterial Agents for Bronchitis: A Network Meta-Analysis. Journal of Cellular Biochemistry, 118, 3171-3183. https://doi.org/10.1002/jcb.25951 |
| [16] | Chang, A.B., Upham, J.W., Masters, I.B., et al. (2016) Protracted Bacterial Bronchitis: The Last Decade and the Road Ahead. Pediatric Pulmonology, 51, 225-242. https://doi.org/10.1002/ppul.23351 |
| [17] | Decramer, M., Janssens, W. and Miravitlles, M. (2012) Chronic Obstructive Pulmonary Disease. The Lancet, 379, 1341-1351. https://doi.org/10.1016/S0140-6736(11)60968-9 |
| [18] | Christenson, S.A., Smith, B.M., Bafadhel, M., et al. (2022) Chronic Obstructive Pulmonary Disease. The Lancet, 399, 2227-2242. https://doi.org/10.1016/S0140-6736(22)00470-6 |
| [19] | Ferrera, M.C., Labaki, W.W. and Han, M.K. (2021) Advances in Chronic Obstructive Pulmonary Disease. Annual Review of Medicine, 72, 119-134. https://doi.org/10.1146/annurev-med-080919-112707 |
| [20] | Blanchard, A.C. and Waters, V.J. (2019) Microbiology of Cystic Fibrosis Airway Disease. Seminars in Respiratory and Critical Care Medicine, 40, 727-736. https://doi.org/10.1055/s-0039-1698464 |
| [21] | Ferreira-Coimbra, J., Sarda, C. and Rello, J. (2020) Burden of Community-Acquired Pneumonia and Unmet Clinical Needs. Advances in Therapy, 37, 1302-1318. https://doi.org/10.1007/s12325-020-01248-7 |
| [22] | Veve, M.P. and Wagner, J.L. (2018) Lefamulin: Review of a Promising Novel Pleuromutilin Antibiotic. Pharmacotherapy: The Journal of Human Pharmacology and Drug Therapy, 38, 935-946. https://doi.org/10.1002/phar.2166 |
| [23] | Coates, A.R.M., Hu, Y., Holt, J., et al. (2020) Antibiotic Combination Therapy against Resistant Bacterial Infections: Synergy, Rejuvenation and Resistance Reduction. Expert Review of Anti-Infective Therapy, 18, 5-15. https://doi.org/10.1080/14787210.2020.1705155 |
| [24] | Ho, J. and Ip, M. (2019) Antibiotic-Resistant Community-Acquired Bacterial Pneumonia. Infectious Disease Clinics of North America, 33, 1087-1103. https://doi.org/10.1016/j.idc.2019.07.002 |
| [25] | Ahmad, M. and Khan, A.U. (2019) Global Economic Impact of Antibiotic Resistance: A Review. Journal of Global Antimicrobial Resistance, 19, 313-316. https://doi.org/10.1016/j.jgar.2019.05.024 |
| [26] | Munguia, J., LaRock, D.L., Tsunemoto, H., et al. (2017) The Mla Pathway Is Critical for Pseudomonas aeruginosa Resistance to Outer Membrane Permeabilization and Host Innate Immune Clearance. Journal of Molecular Medicine, 95, 1127-1136. https://doi.org/10.1007/s00109-017-1579-4 |
| [27] | Carniello, V., Peterson, B.W., Van der Mei, H.C., et al. (2018) Physico-Chemistry from Initial Bacterial Adhesion to Surface-Programmed Biofilm Growth. Advances in Colloid and Interface Science, 261, 1-14. https://doi.org/10.1016/j.cis.2018.10.005 |
| [28] | Chaban, B., Hughes, H.V. and Beeby, M. (2015) The Flagellum in Bacterial Pathogens: For Motility and a Whole Lot More. Seminars in Cell & Developmental Biology, 46, 91-103. https://doi.org/10.1016/j.semcdb.2015.10.032 |
| [29] | Zhao, A.L., Sun, J.Z. and Liu, Y.P. (2023) Understanding Bacterial Biofilms: From Definition to Treatment Strategies. Frontiers in Cellular and Infection Microbiology, 13, Article 1137947. https://doi.org/10.3389/fcimb.2023.1137947 |
| [30] | Costerton, J.W., Stewart, P.S. and Greenberg, E.P. (1999) Bacterial Biofilms: A Common Cause of Persistent Infections. Science, 284, 1318-1322. https://doi.org/10.1126/science.284.5418.1318 |
| [31] | Maurice, N.M., Bedi, B. and Sadikot, R.T. (2018) Pseudomonas aeruginosa Biofilms: Host Response and Clinical Implications in Lung Infections. American Journal of Respiratory Cell and Molecular Biology, 58, 428-439. https://doi.org/10.1165/rcmb.2017-0321TR |
| [32] | Siegel, S.J. and Weiser, J.N. (2015) Mechanisms of Bacterial Colonization of the Respiratory Tract. Annual Review of Microbiology, 69, 425-444. https://doi.org/10.1146/annurev-micro-091014-104209 |
| [33] | Muras, A., Otero-Casal, P., Blanc, V., et al. (2020) Acyl Homoserine Lactone-Mediated Quorum Sensing in the Oral Cavity: A Paradigm Revisited. Scientific Reports, 10, Article No. 9800. https://doi.org/10.1038/s41598-020-66704-4 |
| [34] | Tabatabaeifar, F., Isaei, E., Kalantar-Neyestanaki, D., et al. (2022) Antimicrobial and Antibiofilm Effects of Combinatorial Treatment Formulations of Anti-Inflammatory Drugs—Common Antibiotics against Pathogenic Bacteria. Pharmaceutics, 15, Article 4. https://doi.org/10.3390/pharmaceutics15010004 |
| [35] | Weiser, J.N., Ferreira, D.M. and Paton, J.C. (2018) Streptococcus pneumoniae: Transmission, Colonization and Invasion. Nature Reviews Microbiology, 16, 355-367. https://doi.org/10.1038/s41579-018-0001-8 |
| [36] | Naninck, T., Coutte, L., Mayet, C., et al. (2018) In Vivo Imaging of Bacterial Colonization of the Lower Respiratory Tract in a Baboon Model of Bordetella pertussis Infection and Transmission. Scientific Reports, 8, Article No. 12297. https://doi.org/10.1038/s41598-018-30896-7 |
| [37] | Karpurapu, M., Lee, Y.G., Qian, Z, et al. (2018) Inhibition of Nuclear Factor of Activated T Cells (NFAT) C3 Activation Attenuates Acute Lung Injury and Pulmonary Edema in Murine Models of Sepsis. Oncotarget, 9, 10606-10620. https://doi.org/10.18632/oncotarget.24320 |
| [38] | Wang, Q.L., Yang, L., Peng, Y., et al. (2019) Ginsenoside Rg1 Regulates SIRT1 to Ameliorate Sepsis-Induced Lung Inflammation and Injury via Inhibiting Endoplasmic Reticulum Stress and Inflammation. Mediators of Inflammation, 2019, Article ID: 6453296. https://doi.org/10.1155/2019/6453296 |
| [39] | Viana, F., O’Kane, C.M. and Schroeder, G.N. (2021) Precision-Cut Lung Slices: A Powerful ex Vivo Model to Investigate Respiratory Infectious Diseases. Molecular Microbiology, 117, 578-588. https://doi.org/10.1111/mmi.14817 |
| [40] | He, B., Chen, G. and Zeng, Y. (2016) Three-Dimensional Cell Culture Models for Investigating Human Viruses. Virologica Sinica, 31, 363-379. https://doi.org/10.1007/s12250-016-3889-z |
| [41] | Habanjar, O., Diab-Assaf, M., Caldefie-Chezet, F., et al. (2021) 3D Cell Culture Systems: Tumor Application, Advantages, and Disadvantages. International Journal of Molecular Sciences, 22, Article 12200. https://doi.org/10.3390/ijms222212200 |
| [42] | Nickerson, C.A., Richter, E.G. and Ott, C.M. (2006) Studying Host-Pathogen Interactions in 3-D: Organotypic Models for Infectious Disease and Drug Development. Journal of Neuroimmune Pharmacology, 2, 26-31. https://doi.org/10.1007/s11481-006-9047-x |
| [43] | Eenjes, E., Mertens, T.C.J., Buscop-Van Kempen, M.J., et al. (2018) A Novel Method for Expansion and Differentiation of Mouse Tracheal Epithelial Cells in Culture. Scientific Reports, 8, Article No. 7349. https://doi.org/10.1038/s41598-018-25799-6 |
| [44] | Hiemstra, P.S., Tetley, T.D. and Janes, S.M. (2019) Airway and Alveolar Epithelial Cells in Culture. European Respiratory Journal, 54, Article 1900742. https://doi.org/10.1183/13993003.00742-2019 |
| [45] | Aoki, S., Takezawa, T., Sugihara, H., et al. (2016) Progress in Cell Culture Systems for Pathological Research. Pathology International, 66, 554-562. https://doi.org/10.1111/pin.12443 |
| [46] | Luna, C.M., Sibila, O., Agusti, C., et al. (2009) Animal Models of Ventilator-Associated Pneumonia. European Respiratory Journal, 33, 182-188. https://doi.org/10.1183/09031936.00046308 |
| [47] | Zhao, M., Lepak, A.J. and Andes, D.R. (2016) Animal Models in the Pharmacokinetic/Pharmacodynamic Evaluation of Antimicrobial Agents. Bioorganic & Medicinal Chemistry, 24, 6390-6400. https://doi.org/10.1016/j.bmc.2016.11.008 |
| [48] | Kukavica-Ibrulj, I. and Levesque, R.C. (2008) Animal Models of Chronic Lung Infection with Pseudomonas aeruginosa: Useful Tools for Cystic Fibrosis Studies. Laboratory Animals, 42, 389-412. https://doi.org/10.1258/la.2007.06014e |
| [49] | Hraiech, S., Papazian, L., Rolain, J.M., et al. (2015) Animal Models of Polymicrobial Pneumonia. Drug Design, Development and Therapy, 9, 3279-3292. https://doi.org/10.2147/DDDT.S70993 |
| [50] | Bonniaud, P., Fabre, A., Frossard, N., et al. (2018) Optimising Experimental Research in Respiratory Diseases: An ERS Statement. European Respiratory Journal, 51, Article 1702133. https://doi.org/10.1183/13993003.02133-2017 |
| [51] | Bielen, K., Jongers, B., Malhotra-Kumar, S., et al. (2017) Animal Models of Hospital-Acquired Pneumonia: Current Practices and Future Perspectives. Annals of Translational Medicine, 5, Article 132. https://doi.org/10.21037/atm.2017.03.72 |
| [52] | Robinson, N.B., Krieger, K., Khan, F.M., et al. (2019) The Current State of Animal Models in Research: A Review. International Journal of Surgery, 72, 9-13. https://doi.org/10.1016/j.ijsu.2019.10.015 |
| [53] | Reynolds, D. and Kollef, M. (2021) The Epidemiology and Pathogenesis and Treatment of Pseudomonas aeruginosa Infections: An Update. Drugs, 81, 2117-2131. https://doi.org/10.1007/s40265-021-01635-6 |
| [54] | Loebinger, M.R. and Wilson, R. (2008) Bacterial Pneumonia. Medicine, 36, 285-290. https://doi.org/10.1016/j.mpmed.2008.03.011 |
| [55] | Gibson, R.L., Burns, J.L. and Ramsey, B.W. (2003) Pathophysiology and Management of Pulmonary Infections in Cystic Fibrosis. American Journal of Respiratory and Critical Care Medicine, 168, 918-951. https://doi.org/10.1164/rccm.200304-505SO |
| [56] | Diaz, M.H., Shaver, C.M., King, J.D., et al. (2008) Pseudomonas aeruginosa Induces Localized Immunosuppression during Pneumonia. Infection and Immunity, 76, 4414-4421. https://doi.org/10.1128/IAI.00012-08 |
| [57] | Lyczak, J.B., Cannon, C.L. and Pier, G.B. (2000) Establishment of Pseudomonas aeruginosa Infection: Lessons from a Versatile Opportunist1*Address for Correspondence: Channing Laboratory, 181 Longwood Avenue, Boston, MA 02115, USA. Microbes and Infection, 2, 1051-1060. https://doi.org/10.1016/S1286-4579(00)01259-4 |
| [58] | Kerr, K.G. and Snelling, A.M. (2009) Pseudomonas aeruginosa: A Formidable and Ever-Present Adversary. Journal of Hospital Infection, 73, 338-344. https://doi.org/10.1016/j.jhin.2009.04.020 |
| [59] | Wu, D.C., Chan, W.W., Metelitsa, A.I., et al. (2011) Skin Infection Clinical Features, Epidemiology, and Management. American Journal of Clinical Dermatology, 12, 157-169. https://doi.org/10.2165/11539770-000000000-00000 |
| [60] | Wood, S.J., Kuzel, T.M. and Shafikhani, S.H. (2023) Infections, Animal Modeling, and Therapeutics. Cells, 12, Article 199. https://doi.org/10.3390/cells12010199 |
| [61] | Williams, B.J., Dehnbostel, J. and Blackwell, T.S. (2010) Pseudomonas aeruginosa: Host Defence in Lung Diseases. Respirology, 15, 1037-1056. https://doi.org/10.1111/j.1440-1843.2010.01819.x |
| [62] | Gellatly, S.L. and Hancock, R.E.W. (2013) Pseudomonas aeruginosa: New Insights into Pathogenesis and Host Defenses. Pathogens and Disease, 67, 159-173. https://doi.org/10.1111/2049-632X.12033 |
| [63] | Mendes, O.R. (2023) The Challenge of Pulmonary Pseudomonas aeruginosa Infection: How to Bridge Research and Clinical Pathology. In: Bagchi, D., Das, A. and Downs, B.W., Eds., Viral, Parasitic, Bacterial, and Fungal Infections, Academic Press, Cambridge, MA, 591-608. https://doi.org/10.1016/B978-0-323-85730-7.00019-9 |
| [64] | Moore, N.M. and Flaws, M.L. (2011) Antimicrobial Resistance Mechanisms in Pseudomonas aeruginosa. Clinical Laboratory Science, 24, 47-51. https://doi.org/10.29074/ascls.24.1.47 |
| [65] | Rosenthal, V.D., Duszynska, W., Ider, B.-E., et al. (2021) International Nosocomial Infection Control Consortium (INICC) Report, Data Summary of 45 Countries for 2013-2018, Adult and Pediatric Units, Device-Associated Module. American Journal of Infection Control, 49, 1267-1274. https://doi.org/10.1016/j.ajic.2021.04.077 |
| [66] | Moradali, M.F., Ghods, S. and Rehm, B.H.A. (2017) Pseudomonas aeruginosa Lifestyle: A Paradigm for Adaptation, Survival, and Persistence. Frontiers in Cellular and Infection Microbiology, 7, Article 39. https://doi.org/10.3389/fcimb.2017.00039 |
| [67] | Ho, D.-K., Nichols, B.L.B., Edgar, K.J, et al. (2019) Challenges and Strategies in Drug Delivery Systems for Treatment of Pulmonary Infections. European Journal of Pharmaceutics and Biopharmaceutics, 144, 110-124. https://doi.org/10.1016/j.ejpb.2019.09.002 |
| [68] | Diep, B.A., Le, V.T.M., Visram, Z.C., et al. (2016) Improved Protection in a Rabbit Model of Community-Associated Methicillin-Resistant Staphylococcus aureus Necrotizing Pneumonia Upon Neutralization of Leukocidins in Addition to Alpha-Hemolysin. Antimicrobial Agents and Chemotherapy, 60, 6333-6340. https://doi.org/10.1128/AAC.01213-16 |
| [69] | Kurahashi, K., Kajikawa, O., Sawa, T., et al. (1999) Pathogenesis of Septic Shock in Pseudomonas aeruginosa Pneumonia. Journal of Clinical Investigation, 104, 743-750. https://doi.org/10.1172/JCI7124 |
| [70] | Li Bassi, G., Rigol, M., Marti, J.-D., et al. (2014) A Novel Porcine Model of Ventilator-Associated Pneumonia Caused by Oropharyngeal Challenge with Pseudomonas aeruginosa. Anesthesiology, 120, 1205-1215. https://doi.org/10.1097/ALN.0000000000000222 |
| [71] | Moser, K.M., Maurer, J., Jassy, L., et al. (1982) Sensitivity, Specificity, and Risk of Diagnostic Procedures in a Canine Model of Streptococcus pneumoniae Pneumonia. American Review of Respiratory Disease, 125, 436-442. |
| [72] | Thomassen, M.J., Klinger, J.D., Winnie, G.B., et al. (1984) Pulmonary Cellular Response to Chronic Irritation and Chronic Pseudomonas aeruginosa Pneumonia in Cats. Infection and Immunity, 45, 741-747. https://doi.org/10.1128/iai.45.3.741-747.1984 |
| [73] | Kukavica-Ibrulj, I., Facchini, M., Cigana, C., et al. (2014) Assessing Pseudomonas aeruginosa Virulence and the Host Response Using Murine Models of Acute and Chronic Lung Infection. Methods in Molecular Biology, 1149, 757-771. https://doi.org/10.1007/978-1-4939-0473-0_58 |
| [74] | Munder, A. and Tummler, B. (2014) Assessing Pseudomonas Virulence Using Mammalian Models: Acute Infection Model. Methods in Molecular Biology, 1149, 773-791. https://doi.org/10.1007/978-1-4939-0473-0_59 |
| [75] | Mazzolini, R., Rodríguez-Arce, I., Fernández-Barat, L., et al. (2023) Engineered Live Bacteria Suppress Pseudomonas aeruginosa Infection in Mouse Lung and Dissolve Endotracheal-Tube Biofilms. Nature Biotechnology, 41, 1089-1098. https://doi.org/10.1038/s41587-022-01584-9 |
| [76] | Cash, H.A., Woods, D.E., McCullough, B., et al. (1979) A Rat Model of Chronic Respiratory Infection with Pseudomonas aeruginosa. American Review of Respiratory Disease, 119, 453-459. |
| [77] | Nakamura, S., Iwanaga, N., Seki, M., et al. (2016) Toll-Like Receptor 4 Agonistic Antibody Promotes Host Defense against Chronic Pseudomonas aeruginosa Lung Infection in Mice. Infection and Immunity, 84, 1986-1993. https://doi.org/10.1128/IAI.01384-15 |
| [78] | Van Heeckeren, A.M. and Schluchter, M.D. (2002) Murine Models of Chronic Pseudomonas aeruginosa Lung Infection. Laboratory Animals, 36, 291-312. https://doi.org/10.1258/002367702320162405 |
| [79] | Yanagihara, K., Tomono, K., Sawai, T., et al. (1997) Effect of Clarithromycin on Lymphocytes in Chronic Respiratory Pseudomonas aeruginosa Infection. American Journal of Respiratory and Critical Care Medicine, 155, 337-342. https://doi.org/10.1164/ajrccm.155.1.9001333 |
| [80] | Hraiech, S., Bregeon, F., Brunel, J.M., et al. (2012) Antibacterial Efficacy of Inhaled Squalamine in a Rat Model of Chronic Pseudomonas aeruginosa Pneumonia. Journal of Antimicrobial Chemotherapy, 67, 2452-2458. https://doi.org/10.1093/jac/dks230 |
| [81] | Growcott, E.J., Coulthard, A., Amison, R., et al. (2011) Characterisation of a Refined Rat Model of Respiratory Infection with Pseudomonas aeruginosa and the Effect of Ciprofloxacin. Journal of Cystic Fibrosis, 10, 166-174. https://doi.org/10.1016/j.jcf.2010.12.007 |
| [82] | Allewelt, M., Tuomanen, E.I., Coleman, F.T., et al. (2000) Acquisition of Expression of the Pseudomonas aeruginosa ExoU Cytotoxin Leads to Increased Bacterial Virulence in a Murine Model of Acute Pneumonia and Systemic Spread. Infection and Immunity, 68, 3998-4004. https://doi.org/10.1128/IAI.68.7.3998-4004.2000 |
| [83] | Fothergill, J.L., Neill, D.R., Loman, N., et al. (2014) Pseudomonas aeruginosa Adaptation in the Nasopharyngeal Reservoir Leads to Migration and Persistence in the Lungs. Nature Communications, 5, Article No. 4780. https://doi.org/10.1038/ncomms5780 |
| [84] | Morris, A.E., Liggitt, H.D., Hawn, T.R., et al. (2009) Role of Toll-Like Receptor 5 in the Innate Immune Response to Acute P. aeruginosa Pneumonia. American Journal of Physiology-Lung Cellular and Molecular Physiology, 297, L1112-L1119. https://doi.org/10.1152/ajplung.00155.2009 |
| [85] | Yu, H., Hanes, M., Chrisp, C.E., et al. (1998) Microbial Pathogenesis in Cystic Fibrosis: Pulmonary Clearance of Mucoid Pseudomonas aeruginosa and Inflammation in a Mouse Model of Repeated Respiratory Challenge. Infection and Immunity, 66, 280-288. https://doi.org/10.1128/IAI.66.1.280-288.1998 |
| [86] | Facchini, M., De Fino, I., Riva, C., et al. (2014) Long Term Chronic Pseudomonas aeruginosa Airway Infection in Mice. Journal of Visualized Experiments, No. 85, Article 51019. |
| [87] | Hoiby, N., Krogh Johansen, H., Moser, C., et al. (2001) Pseudomonas aeruginosa and the in Vitro and in Vivo Biofilm Mode of Growth. Microbes and Infection, 3, 23-35. https://doi.org/10.1016/S1286-4579(00)01349-6 |
| [88] | Boyd, R.L., Ramphal, R., Rice, R., et al. (1983) Chronic Colonization of Rat Airways with Pseudomonas aeruginosa. Infection and Immunity, 39, 1403-1410. https://doi.org/10.1128/iai.39.3.1403-1410.1983 |
| [89] | Schroeder, T.H., Reiniger, N., Meluleni, G., et al. (2001) Transgenic Cystic Fibrosis Mice Exhibit Reduced Early Clearance of Pseudomonas aeruginosa from the Respiratory Tract. The Journal of Immunology, 166, 7410-7418. https://doi.org/10.4049/jimmunol.166.12.7410 |
| [90] | Thomsen, K., Christophersen, L., Bjarnsholt, T., et al. (2016) Anti-Pseudomonas aeruginosa IgY Antibodies Augment Bacterial Clearance in a Murine Pneumonia Model. Journal of Cystic Fibrosis, 15, 171-178. https://doi.org/10.1016/j.jcf.2015.08.002 |
| [91] | Cheung, D.O., Halsey, K. and Speert, D.P. (2000) Role of Pulmonary Alveolar Macrophages in Defense of the Lung against Pseudomonas aeruginosa. Infection and Immunity, 68, 4585-4592. https://doi.org/10.1128/IAI.68.8.4585-4592.2000 |
| [92] | Cui, Z., Han, D., Sun, X., et al. (2015) Mannose-Modified Chitosan Microspheres Enhance OprF-OprI-Mediated Protection of Mice against Pseudomonas aeruginosa Infection via Induction of Mucosal Immunity. Applied Microbiology and Biotechnology, 99, 667-680. https://doi.org/10.1007/s00253-014-6147-z |
| [93] | De Vrankrijker, A.M., Wolfs, T.F., Ciofu, O., et al. (2009) Respiratory Syncytial Virus Infection Facilitates Acute Colonization of Pseudomonas aeruginosa in Mice. Journal of Medical Virology, 81, 2096-2103. https://doi.org/10.1002/jmv.21623 |
| [94] | Kaushal, D., Miller, M.A., Stabenow, J.M., et al. (2012) Visualization of Murine Intranasal Dosing Efficiency Using Luminescent Francisella tularensis: Effect of Instillation Volume and Form of Anesthesia. PLOS ONE, 7, e31359. https://doi.org/10.1371/journal.pone.0031359 |
| [95] | Sordelli, D.O., Cerquetti, M.C. and Hooke, A.M. (1985) Replication Rate of Pseudomonas aeruginosa in the Murine Lung. Infection and Immunity, 50, 388-391. https://doi.org/10.1128/iai.50.2.388-391.1985 |
| [96] | Evans, S.E., Tuvim, M.J., Zhang, J., et al. (2010) Host Lung Gene Expression Patterns Predict Infectious Etiology in a Mouse Model of Pneumonia. Respiratory Research, 11, Article No. 101. https://doi.org/10.1186/1465-9921-11-101 |
| [97] | Iizawa, Y., Nishi, T., Kondo, M., et al. (1991) Examination of Host Defense Factors Responsible for Experimental Chronic Respiratory Tract Infection Caused by Klebsiella pneumoniae in Mice. Microbiology and Immunology, 35, 615-622. https://doi.org/10.1111/j.1348-0421.1991.tb01593.x |
| [98] | Garcia-Medina, R., Dunne, W.M., Singh, P.K., et al. (2005) Pseudomonas aeruginosa Acquires Biofilm-Like Properties within Airway Epithelial Cell. Infection and Immunity, 73, 8298-8305. https://doi.org/10.1128/IAI.73.12.8298-8305.2005 |
| [99] | Fleiszig, S.M., Evans, D.J., Do, N., et al. (1997) Epithelial Cell Polarity Affects Susceptibility to Pseudomonas aeruginosa Invasion and Cytotoxicity. Infection and Immunity, 65, 2861-2867. https://doi.org/10.1128/iai.65.7.2861-2867.1997 |
| [100] | Darling, K.E.A., Dewar, A. and Evans, T.J. (2004) Role of the Cystic Fibrosis Transmembrane Conductance Regulator in Internalization of Pseudomonas aeruginosa by Polarized Respiratory Epithelial Cells. Cellular Microbiology, 6, 521-533. https://doi.org/10.1111/j.1462-5822.2004.00380.x |
| [101] | Zhu, P., Bu, H., Tan, S., et al. (2020) A Novel Cochlioquinone Derivative, CoB1, Regulates Autophagy in Pseudomonas aeruginosa Infection through the PAK1/Akt1/MTOR Signaling Pathway. The Journal of Immunology, 205, 1293-1305. https://doi.org/10.4049/jimmunol.1901346 |
| [102] | Psoter, K.J., De Roos, A.J., Mayer, J.D., et al. (2015) Fine Particulate Matter Exposure and Initial Pseudomonas aeruginosa Acquisition in Cystic Fibrosis. Annals of the American Thoracic Society, 12, 385-391. https://doi.org/10.1513/AnnalsATS.201408-400OC |
| [103] | Augustin, D.K., Heimer, S.R., Tam, C., et al. (2011) Role of Defensins in Corneal Epithelial Barrier Function against Pseudomonas aeruginosa Traversal. Infection and Immunity, 79, 595-605. https://doi.org/10.1128/IAI.00854-10 |
| [104] | Soong, G., Parker, D., Magargee, M., et al. (2008) The Type III Toxins of Pseudomonas aeruginosa Disrupt Epithelial Barrier Function. Journal of Bacteriology, 190, 2814-2821. https://doi.org/10.1128/JB.01567-07 |
| [105] | Kroken, A.R., Gajenthra Kumar, N., Yahr, T.L., et al. (2022) Exotoxin S Secreted by Internalized Pseudomonas aeruginosa Delays Lytic Host Cell Death. PLOS Pathogens, 18, e1010306. https://doi.org/10.1371/journal.ppat.1010306 |
| [106] | Okuda, J., Hayashi, N., Okamoto, M., et al. (2010) Translocation of Pseudomonas aeruginosa from the Intestinal Tract Is Mediated by the Binding of ExoS to an Na,K-ATPase Regulator, FXYD3. Infection and Immunity, 78, 4511-4522. https://doi.org/10.1128/IAI.00428-10 |
| [107] | Zulianello, L., Canard, C., K？hler, T., et al. (2006) Rhamnolipids Are Virulence Factors That Promote Early Infiltration of Primary Human Airway Epithelia by Pseudomonas aeruginosa. Infection and Immunity, 74, 3134-3147. https://doi.org/10.1128/IAI.01772-05 |
| [108] | Liu, J., Chen, X., Dou, M., et al. (2019) Particulate Matter Disrupts Airway Epithelial Barrier via Oxidative Stress to Promote Pseudomonas aeruginosa Infection. Journal of Thoracic Disease, 11, 2617-2627. https://doi.org/10.21037/jtd.2019.05.77 |
