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探讨鼠李糖乳酸杆菌GKLC1 (Lacticaseibacillus rhamnosus GKLC1)对胃部保护的作用
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Abstract:
过去研究团队于酒精引起之肝损伤动物试验中，观察到益生菌(probiotics)鼠李糖乳酸杆菌GKLC1 (Lacticaseibacillus rhamnosus GKLC1)不仅具护肝效果，同时在该模式下观察到受酒精影响损伤的胃部组织获保护的效果。因此进一步地，本研究探讨益生菌GKLC1对胃部的保健功效。针对幽门螺旋杆菌(Helicobacter pylori)引起的胃溃疡(gastric ulcer)，以体外试验琼脂纸锭扩散法(agar disk diffusion test)测试益生菌GKLC1对于三种不同株H. pylori的抑制效果。针对非类固醇消炎药(non-steroidal anti-inflammatory drugs, NSAIDs)引起的胃溃疡，则以ICR小鼠连续摄入10天阿斯匹林(500 mg/kg/day Aspirin)诱发胃损伤，评估益生菌GKLC1 (高剂量：20.4 mg/kg/day；低剂量：5.12 mg/kg/day)预先补充对胃保护的效果。结果益生菌GKLC1对于标准病原株H. pylori 26695或临床分离的抗药病原株H. pylori v633、H. pylori v1354皆无抑制表现；然而经Aspirin诱导的胃溃疡，益生菌GKLC1则可显著减少胃溃疡的面积、胃黏膜溃疡深度、以及增加胃溃疡治愈力。综合以上结果，L. rhamnosus GKLC1对于胃部机能具保护功效，特别是饮食、药物引起的伤胃保护，对于微生物引起的胃病则较无效果。
In the past, the research team observed in animal experiments on alcohol-induced liver damage that the probiotic Lactobacillus rhamnosus GKLC1 (Lacticaseibacillus rhamnosus GKLC1) not only had a liver-protective effect, but also observed less gastric damage caused by alcohol in this model. Therefore, this study further explores the protective effect of probiotic GKLC1 on the stomach. For gastric ulcers caused by Helicobacter pylori, the in vitro agar disk diffusion test was used to test the inhibitory effect of probiotic GKLC1 on three different strains of H. pylori. For gastric ulcers caused by non-steroidal anti-inflammatory drugs (NSAIDs), ICR mice were used to continuously ingest aspirin (500 mg/kg/day) for 10 days to induce gastric damage and evaluate the gastro protective effect of pre-supplementation of probiotics GKLC1 (high dose: 20.4 mg/kg/day; low dose: 5.12 mg/kg/day). Results showed that probiotics GKLC1 had no inhibitory effect on the standard pathogen strain H. pylori 26695 or the clinically isolated drug-resistant pathogen strains H. pylori v633 and H. pylori v1354. However, probiotic GKLC1 could significantly reduce gastric ulcer induced by Aspirin including reducing area of ulcers, the depth of gastric mucosal ulcers, and improving the healing ability of gastric ulcers. Based on the above results, L. rhamnosus GKLC1 has a protective effect on gastric function, especially on gastric injuries caused by diet and drugs, but is less effective on gastric diseases caused by microorganisms.
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