|
|
两株萘降解菌的降解特性及动力学分析
|
Abstract:
研究不同萘初始浓度条件下，铜绿假单胞菌ZS1和红球菌b1对萘的降解特性。ZS1和b1菌株最适萘初始质量浓度为分别为200 mg/L和300 mg/L。培养120 h菌株ZS1和b1对萘的降解率分别达到80.45%和87.13%。通过动力学拟合，证实两种菌株对萘的降解过程均符合一级降解动力学方程，降解常数K分别为0.01843 kd/h？1和0.02832 kd/h？1。通过气相色谱–质谱(GC-MS)分析了萘降解阶段的中间产物，并推测了两种菌株降解萘的途径分别为邻苯二甲酸途径和水杨酸途径。
The degradation characteristics of naphthalene by Pseudomonas aeruginosa ZS1 and Rhodococcus b1 under different initial concentrations of naphthalene were studied. The optimum initial naphthalene concentrations of ZS1 and b1 were 200 mg/L and 300 mg/L, respectively. After 120 hours of culture, the degradation rate of naphthalene by strain ZS1 and b1 reached 80.45% and 87.13%, respectively. Through kinetic fitting, it was confirmed that the degradation process of naphthalene by the two strains conformed to the first-order degradation kinetic equation, and the degradation constants K were 0.01843 kd/h？1 and 0.02832 kd/h？1, respectively. The intermediate products of naphthalene degradation stage were analyzed by gas chromatography-mass spectrometry (GC-MS), and the pathways of naphthalene degradation by the two strains were speculated to be phthalic acid pathway and salicylic acid pathway.
| [1] | Feng, X., Deng, M., Yu, J., et al. (2024) Highly Efficient Removing Polycyclic Aromatic Hydrocarbons in Coking Wastewater by Bio-Augmentation Activated Sludge with Nocardioides sp. JWJ-L0 through Sodium Acetate Co-Metabolism. Journal of Water Process Engineering, 58, Article 104844. https://doi.org/10.1016/j.jwpe.2024.104844 |
| [2] | Yang, H., Qian, Z., Liu, Y., et al. (2024) Comparative Genomics Reveals Evidence of Polycyclic Aromatic Hydrocarbon Degradation in the Moderately Halophilic Genus Pontibacillus. Journal of Hazardous Materials, 462, Article 132724. https://doi.org/10.1016/j.jhazmat.2023.132724 |
| [3] | 许琪. 一株石油源Bacillus firmus对蒽的降解特性及蛋白组学研究[D]: [硕士学位论文]. 沈阳: 辽宁大学, 2022. |
| [4] | 郑淑奇. 四株红球菌的分类鉴定及其降解多环芳烃的途径[D]: [硕士学位论文]. 呼和浩特: 内蒙古大学, 2022. |
| [5] | Huang, H., Wu, K., Khan, A., et al. (2016) A Novel Pseudomonas gessardii Strain LZ-E Simultaneously Degrades Naphthalene and Reduces Hexavalent Chromium. Bioresource Technology, 207, 370-378. https://doi.org/10.1016/j.biortech.2016.02.015 |
| [6] | 王岩. 近海沉积物中几种多环芳烃的生物降解研究[D]: [博士学位论文]. 青岛: 中国海洋大学, 2011. |
| [7] | Li, S.X., Ji, F.L., Lv, J., et al. (2024) Removal of PAHs in Paddy Fields: How Natural Periphytic Biofilm Enhances the Removal Process and the Mechanisms Involved. Environmental Technology & Innovation, 33, Article 103533. https://doi.org/10.1016/j.eti.2024.103533 |
| [8] | 郭亚男, 张馨予, 胥梦, 等. 低温萘降解菌的筛选、鉴定及降解条件优化[J]. 生物技术通报, 2019, 35(7): 100-107. |
| [9] | Maryam, F., Mohammadsaeed, S. and Amir, M.G. (2023) Potential Biodegradation of Polycyclic Aromatic Hydrocarbons (PAHs) and Petroleum Hydrocarbons by Indigenous Fungi Recovered from Crude Oil-Contaminated Soil in Iran. Scientific Reports, 13, Article No. 22153. https://doi.org/10.1038/s41598-023-49630-z |
| [10] | 郑兰健. 高产生物表面活性剂菌株的筛选及其对多环芳烃降解性能的研究[D]: [硕士学位论文]. 长春: 长春工业大学, 2022. |
| [11] | 邹晓爽. 苯并[a]芘好氧降解菌Acinetobacter XS-4的分离筛选、降解性能及机理研究[D]: [硕士学位论文]. 贵阳: 贵州大学, 2022. |
| [12] | 梁雪涛, 苏俊杰, 王震, 等. 两株菲降解菌的降解特性及动力学[J]. 净水技术, 2020, 39(S2): 40-48. |
| [13] | 张惠. 石油降解菌的分离鉴定及对芳香烃萘的协同降解性能的初步研究[D]: [硕士学位论文]. 合肥: 安徽建筑大学, 2020. |
