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脲酶诱导碳酸钙沉积关键影响因素研究
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Abstract:
黄土颗粒间黏结力不足是导致土壤侵蚀流失的重要原因，酶诱导方解石沉积(EICP)技术因其方法简便、胶结强度高、对环境友好等特点迅速在国内发展起来。本文通过研究EICP中脲酶活性、CaCO3生成量与影响因素之间的关系，基于五因素五水平正交试验分析了各因素对脲酶活性及碳酸钙生成量的影响规律。结果表明：脲酶活性与碳酸钙生成量呈线性增加；当胶结液浓度在0.8~1.4 mol/L时，脲酶活性与碳酸钙含量出现较大幅度增长；通过正交实验极差与方差分析得到，最佳影响因素组合为脲酶浓度100 g/L，胶结液浓度1.4 mol/L，脱脂奶粉浓度6 g/L，pH 值8，培养时间5天。
Insufficient intergranular adhesion of loess is an important cause of soil erosion and loss. Enzyme Induced Calcium Carbonate Precipitation (EICP) technology has developed rapidly in China due to its simple method, high cementation strength and environmental friendliness. In this paper, the relationship between urease activity, CaCO3 generation and influencing factors in EICP was studied, and the influence rules of each factor on urease activity and calcium carbonate generation were analyzed based on five-factor and five-level orthogonal test. The results show that: Urease activity increased linearly with calcium carbonate production. Urease activity and calcium carbonate content increased significantly when the concentration of cementing fluid was 0.8~1.4 mol/L. Through orthogonal experiment range and variance analysis, the optimal combination of influencing factors was urease concentration of 100 g/L, cementation fluid concentration of 1.4 mol/L, defatted milk powder concentration of 6 g/L, pH value of 8, and culture time of 5 days.
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