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水稻类病斑突变体基因克隆及其抗病机制
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Abstract:
水稻是重要的粮食作物，也是功能基因组学研究的模式植物，大部分水稻类病斑突变体都表现出过敏性的坏死病变，并对各种病原体的抗性增强，是研究植物免疫机制的理想材料，探究水稻类病斑突变体及其抗病机理是当前的研究热点之一。本研究阐述了水稻类病斑突变体的概念、类病斑基因的克隆与功能以及类病斑突变体的抗病机制，以期为水稻抗病育种提供理论依据。
Rice is an important cereal crop and a model plant for functional genomics research. Most rice lesion mimic mutants lines exhibit hypersensitive necrotic lesions and enhanced resistance to various pathogens, making them ideal materials for studying plant immune mechanisms. Exploring rice lesion mimic mutants and their resistance mechanisms is one of the current research hotspots. This study elaborates on the concept of rice lesion mimic mutants, the cloning and function of blast-related genes, and the disease resistance of blast mutants, aiming to provide a theoretical basis for rice disease-resistant breeding.
| [1] | Liu, W.D., Liu, J.L., Triplett, L., et al. (2013) Novel Insights into Rice Innate Immunity against Bacterial and Fungal Pathogens. Annual Review of Phytopathology, 52, 213-241. https://doi.org/10.1146/annurev-phyto-102313-045926 |
| [2] | Yuan, Y.X., Zhong, S.H., Li, Q., et al. (2007) Functional Analysis of Rice NPR1-Like Genes Reveals That OsNPR1/NH1 Is the Rice Orthologue Conferring Disease Resistance with Enhanced Herbivore Susceptibility. Plant Biotechnology Journal, 5, 313-324. https://doi.org/10.1111/j.1467-7652.2007.00243.x |
| [3] | Jiang, C.J., Shimono, M., Maeda, S., et al. (2009) Suppression of the Rice Fatty-Acid Desaturase Gene OsSSI2 Enhances Resistance to Blast and Leaf Blight Diseases in Rice. Molecular Plant-Microbe Interactions, 22, 820-829. https://doi.org/10.1094/MPMI-22-7-0820 |
| [4] | Undan, J.R., Tamiru, M., Abe, A., et al. (2012) Mutation in OsLMS, a Gene Encoding a Protein with Two Double-Stranded RNA Binding Motifs, Causes Lesion Mimic Phenotype and Early Senescence in Rice (Oryzasativa L.). Genes & Genetic Systems, 87, 169-179. https://doi.org/10.1266/ggs.87.169 |
| [5] | Wang, L.J., Pei, Z.Y., Tian, Y.C. and He, C.X. (2005) OsLSD1 A Rice Zinc Finger Protein, Regulates Programmed Cell Death and Callus Differentiation. Molecular Plant-Microbe Interactions, 18, 375-384. https://doi.org/10.1094/MPMI-18-0375 |
| [6] | Sun, C.H., Liu, L.C., Tang, J.Y., et al. (2011) RLIN1, Encoding a Putative Coproporphyrinogen III Oxidase, Is Involved in Lesion Initiation in Rice. Journal of Genetics and Genomics, 38, 29-37. https://doi.org/10.1016/j.jcg.2010.12.001 |
| [7] | Chen, X.F., Hao, L., Pan, J.W., et al. (2012) SPL5, A Cell Death and Defense-Related Gene, Encodes a Putative Splicing Factor 3 B Subunit 3 (SF3b3) in Rice. Molecular Breeding, 30, 939-949. https://doi.org/10.1007/s11032-011-9677-4 |
| [8] | Rao, Y.C., Jiao, R., Wang, S., et al. (2021) SPL36 Encodes a Receptor-Like Protein Kinase That Regulates Programmed Cell Death and Defense Responses in Rice. Rice, 14, Article No. 34. https://doi.org/10.1186/s12284-021-00475-y |
| [9] | Ma, J.Y., Chen, S.L., Zhang, J.H., et al. (2012) Identification and Genetic Mapping of a Lesion Mimic Mutant Rice. Rice Science, 19, 1-7. https://doi.org/10.1016/S1672-6308(12)60013-4 |
| [10] | Kiyosawa, S. (1970) Inheritance of a Particular Sensitivity of the Ricevariety, Sekiguchi-Asahi, to Pathogens and Chemicals, and Linkage Relationship with Blast Resistance. Bulletin of the National Institute of Agricultural Sciences. Series D: Plant physiology, genetics and crops in general, 21, 61-71. |
| [11] | Shirsekar, G.S., Vega-Sanchez, M.E., Bordeos, A., et al. (2014) Identification and Characterization of Sup-Pressor Mutants of Spl11-Mediated Cell Death in Rice. Molecular Plant-Microbe Interactions, 27, 528-536. https://doi.org/10.1094/MPMI-08-13-0259-R |
| [12] | 沈旺鑫, 史小品, 杜海波, 等. 水稻类病斑突变体基因克隆及发生机制研究进展[J]. 江苏农业学报, 2022, 38(3): 837-848. |
| [13] | 焦然, 徐娜, 胡娟, 等. 水稻类病变突变体性状及分子机理研究进展[J]. 中国水稻科学, 2018, 32(3): 285-295. |
| [14] | Yamanouchi, U., Yano, M., Lin, H., et al. (2002) A Rice Spotted Leaf Gene, Spl7, Encodes a Heat Stress Transcription Factor Protein. Proceedings of the National Academy of Sciences of the United States of America, 99, 7530-7535. https://doi.org/10.1073/pnas.112209199 |
| [15] | Qiao, Y., Jiang, W., Lee, J., et al. (2010) SPL28 Encodes a Clathrin-Associated Adaptor Protein Complex 1, Medium Subunit Micro 1 (AP1M1) and Is Responsible for Spotted Leaf and Early Senescence in Rice (Oryza sativa). New Phytologist, 185, 258-274. https://doi.org/10.1111/j.1469-8137.2009.03047.x |
| [16] | Sun, L., Wang, Y., Liu, L.L., et al. (2017) Isolation and Characterization of a Spotted Leaf 32 Mutant with Early Leaf Senescence and Enhanced Defense Response in Rice. Scientific Reports, 31, Article No. 41846. https://doi.org/10.1038/srep41846 |
| [17] | Wang, S., Lei, C., Wang, J., et al. (2017) SPL33, Encoding an EEF1A-Like Protein, Negatively Regulates Cell Death and Defense Responses in Rice. Journal of Experimental Botany, 68, 899-913. https://doi.org/10.1093/jxb/erx001 |
| [18] | 伍豪. 水稻类病斑基因LLM1和LLM3的克隆与功能解析[D]: [博士学位论文]. 杭州: 浙江大学, 2023. |
| [19] | Chen, Z., Chen, T., Sathe, A.P., et al. (2018) Identification of a Novel Semi-Dominant Spotted-Leaf Mutant with Enhanced Resistance to Xanthomonas oryzae Pv. Oryzae in Rice. International Journal of Molecular Sciences, 19, Article 3766. https://doi.org/10.3390/ijms19123766 |
| [20] | Danon, A., Miersch, O., Felix, G., et al. (2005) Concurrent Activation of Cell Death-Regulating Signaling Pathways by Singlet Oxygen in Arabidopsis Thaliana. The Plant Journal, 41, 68-80. https://doi.org/10.1111/j.1365-313X.2004.02276.x |
| [21] | Zhang, P., Ma, X., Liu, L., et al. (2023) Mediator Subunit 16 Negatively Regulates Rice Immunity by Modulating Pathogenesis Related 3 Activity. Plant Physiology, 192, 1132-1150. https://doi.org/10.1093/plphys/kiad120 |
| [22] | Lin, A., Wang, Y., Tang, J., et al. (2011) Nitric Oxide and Protein S-Nitrosylation Are Integral to Hydrogen Peroxide-Induced Leaf Cell Death in Rice. Plant Physiology, 158, 451-464. https://doi.org/10.1104/pp.111.184531 |
