Gomphrena serrata L. (Amaranthaceae) is a medicinal plant widely used traditionally in rural areas to treat many diseases, including gastrointestinal helminthiasis of small ruminants. This work aims to estimate the plant extracts phytochemical potential and study the activities of the aqueous decoction (GS_AD), aqueous macerate (GS_AM), and hydroalcoholic macerate (GS_HEM) of Gomphrena serrata extracts on two developmental stages (eggs and adult worms) of Haemonchus contortus. Phytochemical screening was carried out. The acute toxicity test was performed according to the Organisation for Economic Co-operation and Development (OECD) test guideline 423. The Haemonchus contortuseggs hatching inhibition test was performed left to incubate for 48 hours in the presence of the different plant extracts at increasing concentrations of 0.125; 0.25; 0.5; 0.625; 1; 2; 3 mg/mL. For the motility inhibition test on adult worms, the worms were contacted with the extracts at concentrations of 0.75; 1; 1.25; 1.5; 2; 3; 4 mg/mL and left to incubate for 24 hours. Albendazole and levamisole were used as standard. Phytochemical screening revealed the presence of tannins, saponins, reducing compounds, coumarins and derivatives, anthocyanins, steroids, triterpenes, and flavonoids. An acute toxicity study showed that the oral LD50 value of G. serratawas estimated to be 5000 mg/kg. All extracts showed an effect on both stages of parasite development. The hatching inhibition rate was 100% from 3 mg/mL for all three extracts. The rate of inhibition of motility was 100% from 4 mg/mL for each extract. HEM is more efficient on eggs and Haemonchus contortus adult worms than other extracts with IC50 of 0.457 mg/mL and LC50of 1.329 mg/mL, respectively. These results suggest that Gomphrena serrata, a plant used by farmers, has anthelmintic properties.
References
[1]
Morel, P.-C., Itard, J. and Chartier, C. (2000) Précis de parasitologie vétérinaire tropicale. Lavoisier Tech & Doc (Collection Universités francophones), Paris, 796 p.
[2]
Roeber, F., Jex, A.R. and Gasser, R.B. (2013) Impact of Gastrointestinal Parasitic Nematodes of Sheep, and the Role of Advanced Molecular Tools for Exploring Epidemiology and Drug Resistance—An Australian Perspective. Parasites & Vectors, 6, Article No. 153. https://doi.org/10.1186/1756-3305-6-153
[3]
Hoste, H., Meza-Ocampos, G., Marchand, S., Sotiraki, S., Sarasti, K., Blomstrand, B.M., et al. (2022) Use of Agro-Industrial by-Products Containing Tannins for the Integrated Control of Gastrointestinal Nematodes in Ruminants. Parasite, 29, Article No. 10. https://doi.org/10.1051/parasite/2022010
[4]
Laha, R., Das, M. and Goswami, A. (2013) Gastrointestinal Parasitic Infections in Organized Cattle Farms of Meghalaya. Veterinary World, 6, 109-112. https://doi.org/10.5455/vetworld.2013.109-112
[5]
Renwal, K.K., Gupta, A., Kumar, N., Pilania, P.K. and Manohar, G.S. (2017) Prevalence and Risk Assessment of Gastrointestinal Helminthoses in Dairy Animals of Bikaner, Rajasthan. Journal of Parasitic Diseases, 41, 557-561. https://doi.org/10.1007/s12639-016-0850-x
[6]
Chiejina, S.N., Behnke, J.M., Musongong, G.A., Nnadi, P.A. and Ngongeh, L.A. (2010) Resistance and Resilience of West African Dwarf Goats of the Nigerian Savanna Zone Exposed to Experimental Escalating Primary and Challenge Infections with Haemonchus contortus. Veterinary Parasitology, 171, 81-90. https://doi.org/10.1016/j.vetpar.2010.03.007
[7]
Attindehou, S. and Salifou, S. (2012) Epidemiology of Cestodes Infections in Sheep and Goats in Benin. Veterinary Research, 5, 59-62.
[8]
Djawe, P.B., Salhine, R., Bamia, A., Saotoing, P. and Maiwore, J. (2019) Caractérisation des helminthes gastro-intestinaux de bovins dans le département de la Vina. International Journal of Advanced Research in Biological Sciences, 6, 44-53.
[9]
Sheferaw, D., Mohammed, A. and Degefu, A. (2021) Distribution and Prevalence of Gastrointestinal Tract Nematodes of Sheep at Highland and Midland Areas, Ethiopia. Journal of Parasitic Diseases, 45, 995-1001. https://doi.org/10.1007/s12639-021-01397-8
[10]
Lalchhandama, K. (2010) In vitro Effects of Albendazole on Raillietina echinobothrida, the Cestode of Chicken, Gallus domesticus. Journal of Young Pharmacists, 2, 374-378. https://doi.org/10.4103/0975-1483.71630
[11]
Tariq, K.A. and Tantry, M.A. (2012) Preliminary Studies on Plants with Anthelmintic Properties in Kashmir—The North-West Temperate Himalayan Region of India. Chinese Medicine, 3, 106-112. https://doi.org/10.4236/cm.2012.32017
[12]
Coles, G.C., Jackson, F., Pomroy, W.E., Prichard, R.K., Von Samson-Himmelstjerna, G., Silvestre, A., et al. (2006) The Detection of Anthelmintic Resistance in Nematodes of Veterinary Importance. Veterinary Parasitology, 136, 167-185. https://doi.org/10.1016/j.vetpar.2005.11.019
[13]
Khamesipour, F., Kheyri, P., Shojaat, S., Chelgerdi Dehkordi, B., Basirpour, B., Afzal, S.S. et al. (2021) A Review of the Effect of Medicinal Plant on Helminthic Infections. Infectious Diseases and Herbal Medicine, 2, Article 127. https://doi.org/10.4081/idhm.2021.127
[14]
Githiori, J.B., Athanasiadou, S. and Thamsborg, S.M. (2006) Use of Plants in Novel Approaches for Control of Gastrointestinal Helminths in Livestock with Emphasis on Small Ruminants. Veterinary Parasitology, 139, 308-320. https://doi.org/10.1016/j.vetpar.2006.04.021
[15]
Santa Rosa, S.S., Santos, F.O., Lima, H.G., Reis, I.M.A., Cassiano, D.S.A., Vieira, I.J.C., et al. (2018) In vitro Anthelmintic and Cytotoxic Activities of Extracts of Persea willdenovii Kosterm (Lauraceae). Journal of Helminthology, 92, 674-680. https://doi.org/10.1017/S0022149X17000979
[16]
Toklo, P.M., Ladekan, E.Y., Linden, A., Hounzangbe-Adote, S., Kouam, S.F. and Gbenou, J.D. (2021) Anthelmintic Flavonoids and Other ComPounds from Combretum glutinosum Perr. ex DC (Combretaceae) Leaves. Acta Crystallographica Section C: Structural Chemistry, 77, 505-512. https://doi.org/10.1107/S2053229621007841
[17]
Gnoula, C., Guissou, P., Duez, P., Frédérich, M. and and Dubois, J. (2007) Nematocidal Compounds from the Seeds of Balanites aegyptiaca Isolation and Structure Elucidation. International Journal of Pharmacology, 3, 280-284. https://doi.org/10.3923/ijp.2007.280.284
[18]
Belemlilga, M.B., Traoré, T.K., Boly, G.A.L., Ouédraogo, N., Traoré, A., Lompo, M., et al. (2019) Evaluation of Antioxidant, Anti-Inflammatory and Analgesic Activities of Leaves of Saba senegalensis (A.DC) Pichon (Apocynaceae). European Journal of Medicinal Plants, 27, 1-12. https://doi.org/10.9734/ejmp/2019/v27i330116
[19]
Traore, A., Ouédraogo, S., Belemlilga, M.B., Kaboré, A., Guissou, I.P., Belemlilga, B.M., et al. (2017) Phytochemical Analysis and Ovicidal Activity of Cassia Sieberiana, Guiera senegalensis and Excoecaria grahamii Extracts. African Journal of Pharmacy and Pharmacology, 11, 554-560. https://doi.org/10.5897/AJPP2017.4837
[20]
Tianhoun, D.F., Meda, N.-T.R., Konate, A., Kabore, A., Tamboura, H.H. and Belem, A.M.G. (2020) Phytochemical Screening and In vitro Anthelmintic Activity of Cassia alata (L) Roxb. on Haemonchus contortus of Small Ruminants in Burkina Faso. The Journal of Advances in Parasitology, 7, 14-19. https://doi.org/10.17582/journal.jap/2020/7.3.14.19
[21]
Hoste, H., Martinez-Ortiz-De-Montellano, C., Manolaraki, F., Brunet, S., Ojeda-Robertos, N., Fourquaux, I., et al. (2012) Direct and Indirect Effects of Bioactive Tannin-Rich Tropical and Temperate Legumes against Nematode Infections. Veterinary Parasitology, 186, 18-27. https://doi.org/10.1016/j.vetpar.2011.11.042
[22]
Gomes, D.C., de Lima, H.G., Vaz, A.V., Santos, N.S., Santos, F.O., Dias, Ê.R., et al. (2016) In vitro Anthelmintic Activity of the Zizyphus joazeiro Bark against Gastrointestinal Nematodes of Goats and Its Cytotoxicity on Vero Cells. Veterinary Parasitology, 226, 10-16. https://doi.org/10.1016/j.vetpar.2016.06.004
[23]
Houngnimassou, H.M.A., Attindehou, S., Salifou, S., Koumodji, K.D. and Salifou, S. (2017) Effets Strongylicides in vitro de l’extrait aqueux de feuilles de Ficus exasperata Valh. 1805 (Moraceae). International Journal of Biological and Chemical Sciences, 11, 1012-1020. https://doi.org/10.4314/ijbcs.v11i3.6
[24]
Nacoulma, O.G. (1996) Plantes médicinales et Pratiques médicales Traditionnelles: Cas du plateau central. These d’Etat, Université de Ouagadougou, Ouagadougou.
[25]
Nandini, K., Palaksha, M. and Gnanasekaran, D. (2018) A Review of Gomphrena serrata. International Journal of Science and Research Methodology, 11, 105-110.
[26]
Ciulei, I. (1982) Pratical Manuals on the Indistrial Utilization of Chemical and Aromatic Plants. Methodology for Analysis of Vegetable Drugs, Ministry of Chemical Industry B.
[27]
OECD (2002) Test No. 423: Acute Oral Toxicity—Acute Toxic Class Method. Oecd Guideline for Testing of Chemicals Section 4, OECD, 1-14. https://doi.org/10.1787/9789264071001-en
[28]
Ouedraogo, G.G., Ouedraogo, M., Lamien-Sanou, A., Lompo, M., Goumbri-Lompo, O.M. and Guissou, P.I. (2013) Acute and Subchronic Toxicity Studies of Roots Barks Extracts of Calotropis procera (Ait.) R. Br Used in the Treatment of Sickle Cell Disease in Burkina Faso. British Journal of Pharmacology and Toxicology, 5, 194-200. https://doi.org/10.19026/bjpt.4.5401
[29]
Magnini, R.D., Nitiéma, M., Ouédraogo, G.G., Ilboudo, S., Bancé, A., Millogo-Koné, H., et al. (2021) Toxicity and Bacterial Anti-Motility Activities of the Hydroethanolic Extract of Acacia senegal (L.) Willd (Fabaceae) Leaves. BMC Complementary Medicine and Therapies, 21, Article No. 178. https://doi.org/10.1186/s12906-021-03348-5
[30]
Angelo, B.F.D., Wabo, P.J., Yondo, J., Komtangi, C.M., Vittori, S. and Mpoame, M. (2014) Evaluation of Ovicidal and Larvicidal Activities of Methylene Chloride-Methanol Extract of Annona senegalensis (Annonaceae) Stem Bark on Heligmosomoides bakeri (Nematoda, Heligmosomatidae). Global Journal of Science Frontier Research: C Biological Science, 14, 29-39.
[31]
Belemlilga, M.B., Traoré, A., Ouédraogo, S., Kaboré, A., Tamboura, H.H. and Guissou, I.P. (2016) Anthelmintic Activity of Saba senegalensis (A.DC.) Pichon (Apocynaceae) Extract against Adult Worms and Eggs of Haemonchus contortus. Asian Pacific Journal of Tropical Biomedicine, 6, 945-949. https://doi.org/10.1016/j.apjtb.2016.07.015
[32]
Ouahiba, B., Karima, Y., Narimen, B., Razika, L., Souheila, T., Haddad Djillali, E., et al. (2020) Criblage phytochimique et évaluation du pouvoir antioxydant des feuilles de Myrtus communis L. et Rhamnus alaternus L. phytochemical Screening and Evaluation of the Antioxidant Power of Leaves of Myrtus communis L. and Rhamnus alaternus L. Revue Agrobiologia, 10, 1749-1761.
[33]
Prasanna, M.M., Priyanka, M. and Neelothpala, N. (2017) Physicochemical Evaluation of Leaf of Gomphrena serrata. Advanced Herbal Medicine, 3, 16-25.
[34]
Sangwan, N.S., Farooqi, A.H.A., Shabih, F. and Sangwan, R.S. (2001) Regulation of Essential Oil Production in Plants. Plant Growth Regulation, 34, 3-21. https://doi.org/10.1023/A:1013386921596
[35]
Paolini, J., El Ouariachi, E.M., Bouyanzer, A., Hammouti, B., Desjobert, J.M., Costa, J., et al. (2010) Chemical Variability of Artemisia Herba-Alba Asso Essential Oils from East Morocco. Chemical Papers, 64, 550-556. https://doi.org/10.2478/s11696-010-0051-5
[36]
Krishnaraju, A.V., Rao, T.V.N., Sundararaju, D., Vanisree, M., Tsay, H.-S. and Subbaraju, G.V. (2005) Assessment of Bioactivity of Some Sudanese Medicinal Plants Using Brine Shrimp (Artemia salina) Lethality Assay. International Journal of Applied Science and Engineering, 3, 125-134.
[37]
Kharchoufa, L., Bouhrim, M., Bencheikh, N., El Assri, S., Amirou, A., Yamani, A., et al. (2020) Acute and Subacute Toxicity Studies of the Aqueous Extract from Haloxylon scoparium Pomel (Hammada scoparia (Pomel)) by Oral Administration in Rodents. BioMed Research International, 2020, Article ID: 4020647. https://doi.org/10.1155/2020/4020647
[38]
United Nations (2023) Globally Harmonized System of Classification and Labelling of Chemicals (GHS), Tenth Revised Edition United. United Nations, New York and Geneva.
[39]
Ademola, I.O. and Eloff, J.N. (2010) In vitro Anthelmintic Activity of Combretum molle (R. Br. ex G. Don) (Combretaceae) against Haemonchus contortus Ova and Larvae. Veterinary Parasitology, 169, 198-203. https://doi.org/10.1016/j.vetpar.2009.12.036
[40]
Leicach, S.R. and Chludil, H.D. (2014) Plant Secondary Metabolites: Structure-Activity Relationships in Human Health Prevention and Treatment of Common Diseases. Studies in Natural Products Chemistry, 42, 267-304. https://doi.org/10.1016/B978-0-444-63281-4.00009-4
[41]
Spiegler, V., Liebau, E. and Hensel, A. (2017) Medicinal Plant Extracts and Plant-Derived Polyphenols with Anthelmintic Activity against Intestinal Nematodes. Natural Product Reports, 34, 627-643. https://doi.org/10.1039/C6NP00126B
[42]
Martin, R.J. (1997) Modes of Action of Anthelmintic Drugs. TheVeterinary Journal, 154, 11-34. https://doi.org/10.1016/S1090-0233(05)80005-X
[43]
Molan, A.-L. (2014) Effect of Purified Condensed Tannins from Pine Bark on Larval Motility, Egg Hatching and Larval Development of Teladorsagia circumcincta and Trichostrongylus colubriformis (Nematoda: Trichostrongylidae). Folia Parasitologica, 61, 371-376. https://doi.org/10.14411/fp.2014.036
[44]
Brunet, S., Montellano, C.M.O. de Torres-Acosta, J.F.J., Sandoval-Castro, C.A., Aguilar-Caballero, A.J., Capetillo-Leal, C., et al. (2008) Effect of the Consumption of Lysiloma latisiliquum on the Larval Establishment of Gastrointestinal Nematodes in Goats. Veterinary Parasitology, 157, 81-88. https://doi.org/10.1016/j.vetpar.2008.07.013
[45]
Zabré, G., Kaboré, A., Bayala, B., Katiki, L.M., Costa-Júnior, L.M., Tamboura, H.H., et al. (2017) Comparison of the in Vitro Anthelmintic Effects of Acacia nilotica and Acacia raddiana. Parasite, 24, Article No. 44. https://doi.org/10.1051/parasite/2017044
