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Galt基因编辑小鼠的肠道菌群分析
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Abstract:
目的：本研究旨在分析半乳糖-1-磷酸尿苷转移酶(Galactose-1-Phosphateuridylyltransferase, Galt)基因编辑后对小鼠肠道菌群的影响。方法：基于前期构建的Galt基因编辑小鼠模型，通过16S rRNA基因测序技术，对比野生型(Wild type, WT)和Galt基因编辑小鼠肠道内容物中的菌群组成。结果：结果显示，Galt基因编辑小鼠肠道菌群多样性下降，菌群丰富度减少；物种组成也发生显著性变化。Galt基因编辑组中厚壁菌门占比由69.836%降低45.096%，拟杆菌门占比由15.824%增加到37.672%，变形菌门的相对丰度由1.82%增加到13.175%，脱硫弧菌门的相对丰度由5.42%降低到0.36%。在属水平上，未分类的木楠科属和嗜冷杆菌属相对丰度在Galt基因编辑组中上调，萄球菌属和脱硫弧菌属在WT组中上调。进一步对两组小鼠KEGG功能差异分析，结果显示小鼠碳水化合物运输和代谢，能量产生和转化，氨基酸运输和代谢以及与复制，重组和修复相关的基因方面等功能上存在差异。结论：Galt基因编辑对小鼠肠道菌群的丰富度、组成和功能有影响。
Objective: The purpose of this study was to analyze the effect of Galactose-1-phosphateuridylyltransferase gene knockout on intestinal flora in mice. Methods: The Galt mouse model was constructed by CRISPR/Cas9 technology. The composition of gut microbiota in WT group and Galt group was compared by 16S rRNA gene sequencing. Results: The diversity of intestinal flora decreased, and the richness of flora decreased. At the phylum level, the proportion of Firmicutes and Desulfovibrio in the Galt group decreased, and the proportion of Bacteroidetes and Proteobacteria increased. At the genus level, the relative abundance of unclassified Photinia and Psychrobacter was up-regulated in the Galt group, and the relative abundance of Staphylococcus and Desulfovibrio was up-regulated in the WT group. Further analysis of KEGG functional differences between the two groups of mice showed that there were differences in carbohydrate transport and metabolism, energy production and transformation, amino acid transport and metabolism, and replication in mice. Conclusion: GALT gene knockout affects the richness, composition and function of intestinal flora in mice.
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