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线纹海马Sox9a基因的克隆与表达分析
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Abstract:
Sox9在脊椎动物性腺分化及精巢发育中行使重要功能。为探究线纹海马sox9a的分子特征及表达模式，本研究采用RACE获得了其cDNA全长1979 bp，包含339 bp 5’ UTR、1488 bp的ORF和152 bp 3’ UTR，共编码496个氨基酸；氨基酸多重比对显示，线纹海马sox9具有高度保守的HMG-box结构域；系统进化树分析表明，其与侏儒海马sox9a的亲缘关系最近；RT-qPCR显示，sox9a泛在表达于线纹海马成体的主要组织中，在性腺中呈显著的雄性偏好性高表达于精巢，揭示其可能参与线纹海马精巢的分化及维持。本研究为后续深入研究线纹海马sox9a在性别发育中的功能奠定了基础。
Sox9 plays an important role in gonadal differentiation and testis development in vertebrates. In order to explore the molecular characteristics and expression pattern of sox9a in Hippocampus erectus, RACE was used to obtain its full length cDNA of 1979 bp, including 339 bp 5’UTR, 1488 bp ORF and 152 bp 3’UTR, encoding a total of 496 amino acids. Multiple amino acid comparisons showed that the Hippocampus erectus sox9 had a highly conserved HMG-box domain. Phylogenetic tree analysis showed that it was most closely related to Hippocampus zosterae sox9a. RT-qPCR showed that sox9a was ubiquitously expressed in the main tissues of Hippocampus erectus adults, and showed a significant male preference in the gonads and was highly expressed in the testis, suggesting that sox9a might be involved in the differentiation and maintenance of Hippocampus erectus testis. This study laid a foundation for further research on the role of Hippocampus erectus sox9a in sexual development.
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