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预冻温度对罗非鱼皮胶原纤维海绵结构及性能影响
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Abstract:
本研究以罗非鱼皮为原料，探究预冻温度？20℃ (CFS/？20)、？40℃ (CFS/？40)、？60℃ (CFS/？60)、？80℃ (CFS/？80)以及液氮速冻(CFS/LN)对自组装胶原纤维海绵(CFS)结构及性能的影响。扫描电子显微镜(SEM)和X射线衍射图谱(XRD)结果表明，随着预冻温度降低，罗非鱼皮自组装胶原纤维海绵结构致密度逐渐减小，CFS/？20最为致密，且海绵的纤维直径逐渐变大。通过测定胶原纤维海绵的孔隙率、吸水率和保水率，发现CFS/？20、CFS/？40、CFS/？60、CFS/？80、CFS/LN的孔隙率和吸水率逐渐增大。ATR-FTIR结果显示，CFS/？20到CFS/？80于酰胺A带波数略有增加，表明氢键作用减弱。且随着温度降低，压缩强度减小，机械性能下降，抗酶解性减弱。综上所述，预冻温度对罗非鱼皮胶原纤维海绵的结构及性能具有显著影响。
This study investigates the effect of pre-freezing temperatures (？20°C (CFS/？20), ？40°C (CFS/？40), ？60°C (CFS/？60), ？80°C (CFS/？80), and liquid nitrogen flash freezing (CFS/LN)) on the structure and properties of self-assembled collagen fiber sponge (CFS) derived from tilapia skin. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) results indicate that with decreasing pre- freezing temperature, the structure density of tilapia skin self-assembled collagen fiber sponge gradually decreases, with CFS/？20 being the most dense, and the fiber diameter of the sponge gradually increases. The porosity, water absorption rate, and water retention rate of the collagen fiber sponge increase gradually from CFS/？20 to CFS/？80 and CFS/LN. ATR-FTIR results show a slight increase in the wave number of the amide A band from CFS/？20 to CFS/？80, indicating a weakening of hydrogen bonding. Furthermore, with decreasing temperature, the compressive strength decreases, mechanical properties decline, and resistance to enzymatic degradation weakens. In conclusion, pre-freezing temperature significantly affects the structure and properties of tilapia skin collagen fiber sponge.
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