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Botanical Research 2024
构树雌雄植株外植体组织培养再生能力的差异
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Abstract:
目前关于构树(Broussonetia papyrifera)植株外植体组织培养体系的研究有很多，但是探究构树雌雄株外植体组织培养再生能力的差异的研究鲜有报道。为了研究构树雌雄植株外植体组织培养再生能力的差异性，本实验采集在野外条件下构树雌雄株的外植体，选取构树雌雄株叶片和茎段进行组织培养，观察愈伤组织分化、不定芽的分化和生根情况，实验发现构树雌雄株生殖力差异较大，尤其是不定芽的分化阶段，雌雄株生殖力有很明显的区别，无论是构树叶片还是茎段作为外植体，雄株的生殖力均低于雌，叶片外植体的生殖力大于茎段。这为扩大构树苗木的栽培规模、加快构树苗木生产的产业化提供科学依据及技术保障，为构树生态修复的应用奠定基础。
At present, there are many studies on tissue culture system of Broussonetia papyrifera plant explants, but there are few reports on the difference of tissue culture and regeneration ability between male and female Broussonetia papyrifera plants. In order to study the difference of tissue culture and regeneration ability of male and female Broussonetia papyrifera explants, the explants of male and female Broussonetia papyrifera were collected in the field, and the leaves and stem segments of male and female Broussonetia papyrifera were selected for tissue culture, and the callus differentiation, adventitious bud differentiation and rooting were observed. It was found that the fertility of male and female Broussonetia papyrifera was quite different, especially in the stage of adventitious bud differentiation. This provides scientific basis and technical support for expanding the cultivation scale of Broussonetia papyrifera seedlings and accelerating the industrialization of Broussonetia papyrifera seedlings production, and lays a foundation for the application of Broussonetia papyrifera ecological restoration.
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