Flavonoids extraction from Alpinia zerumbet (Pers.) Burtt et Smith leaves using different procedures

the current study aims to verify the best method for a rapid and efficient extraction of flavonoids from alpinia zerumbet. dried leaves were extracted using distillated water and ethanol 70% by extraction methods of shaking maceration, ultrasonic, microwave and stirring. by the application of tlc and reversed-phase hplc techniques the rutin and kaempferol-3-o-glucuronide were detected. ethanol 70% was more efficient for flavonoids extraction than water. no significant yielding variation was verified for ultrasonic, microwave and stirring methods using ethanol 70% (11 to 14%). relative concentration of rutin and kaempferol-3-o-glucuronide, respectively, was higher by ultrasonic (1.5 and 5.62 mg g-1 dried leaves) and by microwave (1.0 and 6.64 mg g-1 dried leaves) methods using 70% ethanol. rapid and simplified extraction proceeding optimize phytochemical work and acquisition of secondary metabolites.