Valida？？o de método analítico para análise de amitraz em mel utilizando GC/TSD

this paper describes the validation of method for analysis of the acaricide amitraz in honey using n-hexane/acetone extraction, cleanup in cartridge of solid phase extraction (spe) and analysis by gas chromatography with nitrogen-phosphorus detector (gc -tsd). the method can be used to monitor the presence of the pesticide amitraz in honey because of its good analytical parameters, precision (cv <11%) and accuracy (> 80%) in the range of 100-400 ng g-1. the limit set in the european union, by committee for veterinary medicinal products, for amitraz in honey (200 ng g-1) is within this range. the response of the analytical system was linear between 100 and 800 ng ml-1 with correlation coefficient of 0.9996, limit of detection (ld -7 ng g-1) and quantification (lq -100 ng g-1). the sample was fortified at three concentrations 100 ng g-1, 200 ng g-1 and 400 ng g-1, giving an average recovery of 80, 88 and 92% respectively. the validated method was used to assess the quality of honey sold in the city of araraquara/sp, in which there were no residues of amitraz.