Efecto del all-trans ácido retinoico en la producción de citocinas por células mononucleares de sangre periférica (CMSP) en ni？os con desnutrición aguda

objective: to compare the effect of all-trans retinoic acid in the production of cytokines by peripheral blood mononuclear cells (pbmc) among children with moderate malnutrition (dn-m), severe (dn-g) and a control group of children eutrophic (eu). methodology: quasi-experimental research in vitro, to assess the effect of different doses of atra (0, 17, 30 and 50 nm) on the concentration of cytokines (l-8, il-1β, il-6, il-10, tnf-α and il-12p70) in pbmc of 21 children, divided into three groups: seven with moderate malnutrition (dn-m), 7 with severe malnutrition (dn-g) and 7 healthy children (eu). results: although 71% of malnourished children (dn-g and dn-m) showed signs of infection, had a population of leukocytes and lymphocytes lower than the eu. children dn-g reported a concentration of c-reactive protein (crp), significantly higher than the other two groups and simultaneously younger, shorter (t/e) and lower hemoglobin. the concentration of il-1β, il-6, il-8, tnfα and il-10 in pbmc was higher in dn-m than in dn-g and eu. comparison between groups was found that 50 nm of atra concentration of il-8 was significantly lower and the higher il-10 in dn-g compared with the other two groups. manova analyses indicate these differences were attributed to the fact to belonging the group (dn-g) and not the dose of atra (50 nm). analysis of the effect of different doses of atra in each group showed a significant decrease with 50nm of atra of il-8 in dn-g and il-10 in eu. il-12p70 increased with doses of 17 nm in dn-m. conclusion: atra affects the production of cytokines but the main determinant of concentration is the type of malnutrition.