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Evaluation of the effects of carbon dioxide pneumoperitoneum on abdominal wall wound healing in rats undergoing segmental resection and anastomosis of the left colonDOI: 10.1590/S0102-86502012000100011 Keywords: abdominal wall, wound healing, pneumoperitoneum, artificial, tensile strength, rats. Abstract: purpose: to evaluate the influence of carbon dioxide pneumoperitoneum on abdominal wall wound healing in rats. methods: eighty rats underwent laparotomy, segmental left colon resection, and anastomosis. the animals were divided into three experimental groups and one control group: ei = pneumoperitoneum for 30 minutes before laparotomy (n=20); eii = pneumoperitoneum for 30 minutes after abdominal closure (n=20); eiii = pneumoperitoneum for 30 minutes before laparotomy and 30 minutes after abdominal closure (n=20); c = control group, without pneumoperitoneum (n=20). in each group, 10 animals were killed 7 days and 10 animals 14 days postoperatively. a segment of the abdominal wall was resected and subjected to tensile strength testing. another segment of abdominal muscle was used for histopathological analysis; the specimens were fixed in formalin and stained with hematoxylin and eosin. results: there were no differences in histopathology and tensile strength values among animals in the experimental and control groups 7 or 14 days after surgery. conclusion: under the present experimental conditions, carbon dioxide pneumoperitoneum did not interfere with abdominal wall wound healing.
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