Long-term following-up of viability of spleen autotransplants in the Beagle canine model

purpose: to examine the possible late complications of splenectomy or spleen autotransplantation in large laboratory animal model, in which we need non-invasive or minimal-invasive methods for long-term monitoring of the experimental animals. methods: experimental groups of beagle dogs were: non-operated control, sham-operated control, splenectomy, spleen autotransplantation with 5 or 10 spleen-chips taken into the greater omentum (furka's technique). prior to operations, on the 1st postoperative week, monthly till the 6th as well as in the 9th and 12th month, hemorheological examinations were performed. in postoperative 12th month colloid scintigraphy and diagnostic laparoscopy were carried out. at the end of the investigation comparative morphological examinations were performed, too. results: from the 4th-5th postoperative month filtration function of spleen-autotransplants showed particular restoration compared to splenectomy group. however, the functional results did not reach the values of the control or sham-operated groups. sham-operated control's scintigraphy nicely showed activity in the spleen. in spleen autotransplantation-groups scintigraphy indicated well the activity of spleen-chips. during diagnostic laparoscopy spleen-chips with their blood supply were found. histologically, the structure of spleen-autotransplants was similar to normal splenic tissue. conclusions: the autotransplants are regenerated, their functions have been partly restored, and thus spleen autotransplantation may prevent the possible complications of splenectomy. these parameters and the presented investigative protocol are suitable for long-term following-up of viability of the spleen-autotransplants.