Association of mesenchymal stem cells with platelet rich plasma on the repair of critical calvarial defects in mice

purpose: to evaluate the effects of mesenchymal stem cells (msc) from eight mice c57bl/6 gfp+ bone marrows expanded in cultures associated with platelets rich plasma (prp) deriving from another eight mice, in the repair of critical defects in calvarial bone produced in twenty-four adult isogenic mice c57bl/6. methods: the animals were submitted to a cranial defect of 6.0mm in diameter and divided into two equal experimental groups. control group did not receive treatment and the treated group received a msc pellet containing 1.0 x 107 cells/ml associated with 50.0μl of plasma gel containing 1.0 x 109 autologous platelets within the defect. results: in the treated group was observed process of angiogenesis and bone repair better than control group. conclusion: mesenchymal stem cells derived from bone marrow of c57bl/6 gfp+ mice associated with prp gel applied in bone critical defects produced in calvarial contributes positively to the process of bone repair.