Evaluation of umbilical cord mesenchymal stem cell labeling with superparamagnetic iron oxide nanoparticles coated with dextran and complexed with Poly-L-lysine

objective: the objective of this study was to evaluate the effect of the labeling of umbilical cord vein derived mesenchymal stem cells with superparamagnetic iron oxide nanoparticles coated with dextran and complexed to a non-viral transfector agent transfector poly-l-lysine. methods: the labeling of mesenchymal stem cells was performed using the superparamagnetic iron oxide nanoparticles/dextran complexed and not complexed to poly-l-lysine. superparamagnetic iron oxide nanoparticles/dextran was incubated with poly-l-lysine in an ultrasonic sonicator at 37°c for 10 minutes for complex formation superparamagnetic iron oxide nanoparticles/dextran/poly-l-lysine by electrostatic interaction. then, the mesenchymal stem cells were incubated overnight with the complex superparamagnetic iron oxide nanoparticles/dextran/poly-l-lysine and superparamagnetic iron oxide nanoparticles/dextran. after the incubation period the mesenchymal stem cells were evaluated by internalization of the complex superparamagnetic iron oxide nanoparticles/dextran/poly-l-lysine and superparamagnetic iron oxide nanoparticles/dextran by prussian blue stain. cellular viability of labeled mesenchymal stem cells was evaluated by cellular proliferation assay using 5,6-carboxy-fluorescein-succinimidyl ester method and apoptosis detection by annexin v- propidium iodide assay. results: mesenchymal stem cells labeled with superparamagnetic iron oxide nanoparticles/dextran without poly-l-lysine not internalized efficiently the superparamagnetic iron oxide nanoparticles due to its low presence detected within cells. mesenchymal stem cells labeled with the complex superparamagnetic iron oxide nanoparticles/dextran/poly-l-lysine efficiently internalized the superparamagnetic iron oxide nanoparticles due to greater presence in the cells interior. the viability and apoptosis assays demonstrated that the mesenchymal stem cells labeled and not labeled respectively with the superparamagnetic iron oxide nanoparticles