Callus induction and plant regeneration of Ulex europaeus

a callus induction and plant regeneration protocol was developed from leaf and thorn explants for the plant ulex europaeus. explants were incubated on 2% sucrose half-strength murashige and skoog medium (ms) with various combinations of plant growth regulators and antioxidants. the best frequency of callus and shoot formation was obtained with 2,4-dichlorophenoxyacetic acid (2,4-d) 1 mg/l x kinetin (kin) 0.2 mg/l (dk medium; callus induction) and zeatin (z) 1 mg/l (dk medium; shoot induction). both media were supplemented with ascorbic acid 200 mg/l to prevent browning and death of the explants. the regenerated shoots transferred to rooting medium (half-strength ms medium, 2% sucrose) showed rapid growth and development of roots (100%). rooted plantlets were successfully transferred to soil in pots containing a 3:1 mixture of soil and vermiculite.