全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

Gene expression of a putative glutathione S-transferase is responsive to abiotic stress in embryogenic cell cultures of Cyclamen persicum

Keywords: auxin, callus, micropropagation, semisolid medium, suspension culture.

Full-Text   Cite this paper   Add to My Lib

Abstract:

background: cyclamen persicum is an economically important ornamental crop that is propagated exclusively through seeds as vegetative propagation using cuttings is not possible. therefore a micropropagation method through somatic embryogenesis is of high interest; however the method suffers from low reliability concerning quality and quantity of the produced plantlets. a crucial step of the protocol is the removal of plant growth regulators (pgrs) that triggers embryo development. in order to get a better insight in this crucial step of the propagation process, a gene expression analysis has been set up using five different genes of glutathione s-transferases (gst) as these are known to be auxin responsive as well as stress reactive. results: one out of the five genes of glutathione s-transferases (cpgst1) displayed a clear down-regulation 72 hrs after removal of pgrs compared to 4 hrs after, implying auxin responsiveness. however, a more detailed analysis including the time points 0, 4 and 72 hrs revealed an initial strong up-regulation after 4 hrs before it was down-regulated after 72 hrs. in comparison fold-changes of the additional four gst-genes were marginal. comparing cultures on semisolid medium to that in suspension, transcript abundances of cpgst1 were clearly decreased in suspension culture. conclusions: against the initial hypothesis cpgst was not auxin responsive but stress reactive, probably especially indicating drought stress imposed on the cells upon transfer from submerged suspension culture to semisolid medium. mechanical stress caused by shaking of suspensions cultures seemed to be less important.

Full-Text

comments powered by Disqus

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133

WeChat 1538708413