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Gayana. Botánica 2011
Estudios cromosómicos en Acmella bellidioides (Sm.) R.K.Jansen (Asteraceae) del nordeste de ArgentinaDOI: 10.4067/S0717-66432011000100003 Keywords: karyotype, microsporogenesis, meiotic behavior. Abstract: acmella bellidioides is a perennial small herb that naturally grows in north-east argentina, brazil, paraguay and uruguay. the species belongs to section megaglottis and it has a chromosome number of n = 13. it exhibits a wide range of morphological variation that has created difficult in distinguishing it from closely related species of this section. acmella bellidioides was collected in open fields with sandy and stony soils. classical cytogenetic techniques were applied to analyze chromosomes at mitosis, examine behavior during microsporogenesis, and determine its karyotype. acmella bellidioides is diploid with 2n = 2x = 26 small size chromosomes (2.22 - 4.01 μm) and 39.25 μm/genome. its karyotype, 12m + 10sm + 4st is unimodal (a2 = 0.16 / r = 1.80) and slightly asymmetrical (a1 = 0.42 / i = 35.88 / r>2 = 0.54 / ai = 3.69) (3a stebbins category). chromosome pair no. 9 (sm) has a terminal macrosatellite in the short arm. microsporogenesis is normal and produces viable pollen grains (>90%). meiotic behavior is regular. in pmc at diakinesis and metaphase i, 13 bivalents were observed. this is the first karyotypic study in acmella. our record of 2n = 2x = 26 chromosomes agrees with the basic number x = 13 proposed for the genus and for section megaglottis, regarded as most ancestral. this might be regarded, therefore, as the basic karyotypic description for acmella.
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