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Diversidad genética de maracuyá en GUATEMALA revelada por marcadores AFLP

Keywords: passiflora edulis sims, similarity, genetic differentiation, clusters, genetic flow.

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Abstract:

the objectives of this study were to characterize with aflp nine genotypes of passion fruit collected in guatemala, to identify clusters and to evaluate the existing genetic diversity. the study was conducted at instituto de ciencia y tecnología agrícolas (icta), during july 2010/may 2011. a preliminary analysis was conducted in which ten selective primer pairs were amplified and 106 polymorphisms were detected. the selective primer pair e+acg/m+cag, e+aca/m+cta, e+act/m+ctg and e+aac/m+ctt, gave the most information. an average of ten loci per selective amplification was detected. similarity analysis revealed no duplicated genotypes. correspondence and clustering analysis identified two groups. the first one included all accession of p. edulis f. edulis sims and the second one all accessions of p. edulis f. flavicarpa degener. the genetic diversity based on nei′s index for the collection was 0,3160. genetic differentiation (gst) was 0,2542 for the entire collection; 25.42% of the genetic diversity is expressed between groups while the 74,58% is expressed within groups. those results give evidence of the evolutionary proximity of the purple and yellow types of p. edulis sims. the genetic flow was high (nm=1,4670) as expected in an allogamous species in which genetic exchange is facilitated.

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