Validación biológica de la técnica de extracción a campo de estradiol fecal en el gato doméstico: reporte preliminar

the objective of this article was to biologically validate the ¨field method¨ for estradiol fecal extraction in domestic felids by a comparative study of samples obtained in different stages of the estrous cycle. a total at of 40 fecal samples from 7 postpubertal female cats were included. the samples were collected in the estrous (n=22) and interestrous (n=18) stages (defined by behavior and vaginal cytology) during 12 months. the "field extraction method¨ was carried out and then 17？ estradiol was measured by radiommunoassay. fecal estradiol concentration ranged from de 926 to 33320 pg/g wet weight. mean ± sem in estrous and interestrous samples were 11814±2171 pg/g and 3238±474 pg/g (p < 0.01), respectively. longitudinal observation of estradiol values clearly indicated that peaks coincided with cytological and behavioral estrus.