ASYMMETRIC BIS(BIDENTATE) AZINE LIGAND AND TRANSITION METAL COMPLEXES: SYNTHESIS, CHARACTERIZATION, DNA-BINDING AND CLEAVAGE STUDIES AND EXTRACTION PROPERTIES FOR SELECTED METALS AND DICHROMATE ANIONS

new bis (bidentate) azine nnn'n' donor ligand was prepared by linking (1z,1'z)-1,1'-{propane-1,3-diylbis[oxybenzene-4,1-diyl(1z)eth-1-yl-1-ylidene]} dihydra-zine to pyridine-2-carbaldehyde. two kinds of copper(ii) and nickel (ii) complexes with different stoichiometries were prepared. reaction of bis(azine) ligand with cu(ii) and ni(ii) chloride with 1:1 molar ratio gave azine complexes with stoichiometry [m(l)cl2] while 2:1 molar ratio reaction of cu(ii) and ni(ii) chloride with bis(azine) composed binuclear copper complexes with general stoichiometry [m2(l)cl4]. the structures of both bis-azine ligand and its complexes were identified by elemental analysis, infrared spectra, uv-vis electronic absorption spectra and magnetic susceptibility measurements, tga and powder xrd. dna binding and dna cleavage activities of the synthesized metal complexes were examined by using uv-vis titration and agarose gel electrophoresis, respectively. the results indicate that all the complexes bind significantly calf thymus dna and cleavage pbr322 dna. furthermore, the complexing properties of the bis-azine ligand toward selected transition metal cations and dichromate anions were also reported.