Supervivencia de Trypanosoma cruzi en bebidas experimentalmente contaminadas

introduction. trypanosoma cruzi is the causative agent of chagas disease, transmitted primarily by triatomine insects. however, in 2005, oral transmission was documented in countries where the disease is endemic for chagas disease. this trend may also occur in colombia, a situation that motivated epidemiological alerts and the necessity for exploring the risk level of oral, human-to-human infection by t. cruzi. objective. survival times were established for the t. cruzi strain ds using juices involved in the outbreak of lebrija county (cesar, colombia) in 2008. materials and methods. survival of the t. cruzi strain was evaluated as defined by vitality (forward movement) and viability (growth in isolation medium novy, mcneal and nicolle/liver infusion tryptose). this strain was molecularly characterized as tcla, isolated from a patient associated with an outbreak in aguachica county (santander, very near lebrija). its survival was tested in tangerine juice, guava, soursop (guanábana), water and sugar water. results. the t. cruzi strain ds remained vital in mandarin at room temperature for 72 hr, at refrigerated temperatures for 36 hr;, the soursop (guanábana) for 48 hr at room temperature and 384 hr under refrigeration; and guava at both temperatures 24 hr. this strain was viable 2 and 24 hours post-infection in each of the other juices at the two temperature conditions. conclusions: the ds t. cruzi strain survived in all drinks for more than 24 hours post-infection, with a survival time of 384 hr in the juice of soursop (guanábana) under refrigeration.