A new allele of peptidase-B in cattle

electrophoretic analyses of peptidase-b were carried out on red cell hemolysates from holstein, mantiqueira and gyr cattle, using cornstarch, known in brazil as penetrose-30. we describe a new peptidase-b allele, denoted pep-b1, in mantiqueira cattle, belonging to the bos taurus group, which are the result of a cross of native cattle of portuguese origin introduced in brazil during colonial times (16th century) with holstein and caracu cattle. the genetic control of peptidase-b was determined by typing parents and progeny segregating for all three alleles, confirming that peptidase b is controlled by a single autosomal locus with three codominant alleles, denoted pep-b1, pep-b2 and pep-b3 the use of the citrate-phosphate buffer system, at ph 5.9, on 14% gel, under the electrophoretic conditions standardized in this study permitted good visualization of all peptidase-b variants.