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Characterization and lytic activity of Pseudomonas fluorescens phages from sewage

DOI: 10.1590/S1517-83822012000100042

Keywords: pseudomonas fluorescens, phages, rflp, afm, protein analysis.

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Abstract:

pseudomonas fluorescens phages from sewage were tested against p. fluorescens isolates of soil and sewage. the phages were characterized as to host range, morphology, structural proteins and genome fingerprint. of the seven phages isolated, one was found to be abundant in sewage (5.9×107 pfu/ml), having broad host range, and distinct protein and dna profile when compared to the other six phages. dna restriction and protein profiles of the phages and their morphology indicate the diversity in the sewage environment. none of the isolates from the rhizosphere regions of various cultivated soils were susceptible to phages isolated from sewage.

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