Avalia？？o da matura？？o nuclear in vitro de oócitos de gatas domésticas (Felis catus) pré-púberes e púberes

this study assessed the in vitro oocyte nuclear maturation in adult and young domestic cats. fifteen ovaries were used; 10 from adult females and 5 from young females. the oocytes collected by aspiration were quantified and classified. the oocytes classified as excellent and/or regular were grouped (10 oocyte /drop) in culture medium covered with mineral oil in disposable petri dishes. the oocyte were incubated at 38°c and 5% de co2 for 48 hours and then washed with 0,4% hyaluronidase, fixed in methanol/acetic acid and stained with acetic orcein. evaluation of chromosomal configuration of oocytes matured in vitro showed 44,68% of oocyte in metaphase ii in the adult female group and 25,32% in the young donor group, showing that puberty influences oocyte capacity for in vitro development.